LOCUS       sequence-8847-ex       14388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14388)
  AUTHORS   Livet J, Weissman TA, Kang H, Draft RW, Lu J, Bennis RA, Sanes JR, 
            Lichtman JW
  TITLE     Transgenic strategies for combinatorial expression of fluorescent 
            proteins in the nervous system.
  JOURNAL   Nature. 2007 Nov 1. 450(7166):56-62.
  PUBMED    17972876
REFERENCE   2  (bases 1 to 14388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Nov 1. 450(7166):56-62."
COMMENT     Sequence Label: Thy1-Brainbow-2.1 R
FEATURES             Location/Qualifiers
     source          1..14388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(6268..6301)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             6324..7040
                     /codon_start=1
                     /product="humanized Renilla green fluorescent protein"
                     /label=hrGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFGN
                     QLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLRYE
                     DGGLVEIRSDINLIEGMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGVLVG
                     QVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQHETAI
                     AQLTSLGKPLGSLHEWV"
     CDS             7092..7112
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(7152..7170)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    7196..7307
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    7440..7561
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(7684..8397,8398..8400,8401..8403))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      8400..8409
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    8417..8450
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(8471..8504)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      8520..8529
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8524..8533
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8526..9203
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFL"
     CDS             9246..9920
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FL"
     promoter        complement(9977..9995)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    10021..10132
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    10265..10386
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(10501..11214,11215..11217,11218..11220))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      11217..11226
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    11349..11382
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    11397..11430
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(14219..14238)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     14338..14360
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcaggatc gcggccgcga tccccgggcg agctcgaatt cagagaccgg
     2101 gaaccaaact agcctttaaa aaacataagt acaggagcca gcaagatggc tcagtgggta
     2161 aaggtgccta ccagcaagcc tgacagcctg agttcagtcc ccacgaacta cgtggtagga
     2221 gaggaccaac caactctgga aatctgttct gcaaacacat gctcacacac acacacacaa
     2281 atagtataaa caattttaaa tttcatttaa aaataatttg taaacaaaat cattagcaca
     2341 ggttttagaa agagcctctt ggtgacatca agttgatgct gtagatgggg tatcattcct
     2401 gaggacccaa aaccgggtct cagcctttcc ccattctgag agttctctct tttctcagcc
     2461 actagctgaa gagtagagtg gctcagcact gggctcttga gttcccaagt cctacaactg
     2521 gtcagcctga ctactaacca gccatgaaga aacaaggagt ggatgggctg agtctgctgg
     2581 gatgggagtg gagttagtaa gtggccatgg atgtaatgac cccagcaatg ctggctagaa
     2641 ggcatgcctc ctttccttgt ctggagacgg aacgggatca tcttgtactc acagaaggga
     2701 gaacattcta gctggttggg ccaaaatgtg caagttcacc tggaggtggt ggtgcatgct
     2761 tttaactcca gtactcagga ggcagggcca ggtggatctc tgtgagttca agaccagcct
     2821 gcactatgga gagagttttg ggacagccag agttacacag aaaaatcctg gtggaaaatc
     2881 tgaaagaaag agagaaagaa agaaagaaag aaagaaagaa agaaagaaag aaagaaagaa
     2941 agaaagaaag aaagaaagga agaaagaaag aaagagtggc aggcaggcag gcaggaggaa
     3001 ggaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa
     3061 aataggtgcg acttcaagat ccggagttac aagcagaatg cactgtttcc ctaacagggc
     3121 caagtgtttt gagtaactga aggtgggcat gatgcctggg aagcagaaac aagccaggca
     3181 gatgcacccc ttgccttgct tccgaagggc tgcagtagca tggaaaacat ggaaaacaac
     3241 caatccattc cctttgctga tataacaggc tccaaagcca aaacctgtca ctggaggctc
     3301 aagagcagat ctccagccaa gaggcaaagg aatgggggaa gctggagggc ctccctctgg
     3361 ttatccaggc ttctgaaggt tcaagcaaag aaagggttac aaccttaaaa ggagagcgtc
     3421 ccggggtatg ggtagaagac tgctccaccc cgacccccag ggtccctaac cgtcttttcc
     3481 ctgggcgagt cagcccaatc acaggactga gagtgcctct ttagtagcag caagccactt
     3541 cggacaccca aatggaacac ctccagtcag ccctcgccga ccaccccacc ccctccatcc
     3601 ttttccctca gcctccgatt ggctgaatct agagtccctc cctgctcccc cctctctccc
     3661 cacccctggt aaaactgcgg gcttcaggct gggtgcagca actggaggcg ttggcgacca
     3721 ggaggaggct gcagctaggg gagtccaggt gagagcaggc cgacgggagg gacccgcaca
     3781 tgcaaggacc gccgcagggc gaggatgcaa gccttcccca gctacagttt tgggaaagga
     3841 taccagggcg ctcctatatg ggggcgcggg aacctgggga aagaaggtgc tcccaggtcg
     3901 aggtgggaga ggaaggcagt gcggggtcac gggctttctc cctgctaacg gacgctttcg
     3961 aagagtgggt gccggagaga accatgagga aggacatcaa ggacagcctt tggtccccaa
     4021 gctcaaatcg ctttagtggt gcgaatagag ggaggaggtg ggtggcaaac tggagggagt
     4081 ccccagcggt gacctcgtgg ctggctggtg cggggcacca ggtaagaaac gcaatgttgc
     4141 gggaggggac tgggtggcag gcgcggggga ggggaaagct agaaaggatg cgagggagcg
     4201 gaggggggag ggagcggggg aatctcaact ggtagaggaa agttaaaatg aggaaatagc
     4261 atcagggtgg ggttagccaa gccgggcctc agggaaaggg cgcaaagttt gtctgggtgt
     4321 gggcttaggt gggctgggta tgagattcgg ggcgccgaaa acactgctgc gcctctgcca
     4381 aatcacgcta cccctgtatc tagttctgct aggcttctcc agccccagcc ccaattcttt
     4441 tctcagtgtc cccttccctc ccctgaatct caagcccaca ctccctcctc cataacccac
     4501 tgttatcaaa tctaagtcat ttgccaccca acaaccatca ggaggcggaa gcagacggga
     4561 ggagtttgag atcaacttgg gctacatcac gagttccaag ctcaccaagg cttcttaagg
     4621 agaccttgtc tctaaaatta attaattaat taattaatag tcccctttct ctgccacaga
     4681 accttgggat ctggctcctg gtcgcagctc cccccacccc aggctgacat tcactgccat
     4741 agcccatccg gaaatcctag tctattcccc atggatcttg aactgcagag agaatggcag
     4801 agtggcccgc cctgtgcaaa ggatgttcct agcctaggtg gagctcgcga actcggagac
     4861 tgtgcctctc ttgggcaagg acaggctaga cagcctgccg gtgttgagct agggcactgt
     4921 ggggaaggca gagaacctgt gcagggcacg caatgaacac aggaccagaa aactgcagcc
     4981 ctaggaacac tcaagagctg gccatttgca agcatctctg gcctccgtgc ttctcactca
     5041 tgtcccatgt cttatacagg cctctgtggc acctcgcttg cctgatctca tccctagccg
     5101 ttaagctttc tgcatgactt atcacttggg gcataatgct ggatacctac cattttctta
     5161 gaccccatca aaatcctatt tgagtgtacg gttcggagaa ctccttattt atccggtaaa
     5221 tgtcttttac tctgctctca gggagctgag gcaggacatt cctgagatac attgggagag
     5281 gaatacagtt tcaataaaat aataggttgg gtggaggtac atgcctataa tgccaccact
     5341 caggaaatgg tggcagcttc gtgagtttga ggccaaccca agaaacatag tgaaaccctg
     5401 tcagtaaata agtaagcaag tatttgagta tctactatat gctagggctg acctggacat
     5461 taggggtcat cttctgaaca aactagtgct tgagggaggt atttggggtt tttgtttgtt
     5521 taatggatct gaatgagttc cagagactgg ctacacagcg atatgactga gcttaacacc
     5581 cctaaagcat acagtcagac caattagaca ataaaaggta tgtatagctt accaaataaa
     5641 aaaattgtat tttcaagaga gtgtctgtct gtgtagccct ggctgttctt gaactcactc
     5701 tgtagaccag gctggcctgg aaatccatct gcctctgcct ctctgcctct ctgcctctct
     5761 gcctctctct ctgcctctct ctgcctctct ctgcccctct ctgcccctct ctgcccctct
     5821 ctgccgccct ctgccttttg ccctctgccc tctgttctct ggcctctgcc ctctccctgc
     5881 cttctccctc tgcctctgcc tcttgagtgc tggaatcaaa ggtgtgagct ctataggtct
     5941 taagttccag aagaaacgta atgaagtcac ccagcaggag gtgctcaggg acagcaagac
     6001 acacacaccc aggacactag gctcccactt ccttggcttt ctctgagtgg caaaggacct
     6061 taggcagtgt cactccctaa gagaagggga taaagagagg ggctgaggta ttcagtcatg
     6121 tgctccgtgg atctcaagcc ctcaaggtaa atggggaccc acctgtccta ccagctggct
     6181 gacctgtagc tttccccacc acagaatcca agtcggaact cttggcacct agaggatctc
     6241 gactagcgct accggactca gatcgagata acttcgtata gcatacatta tacgaagtta
     6301 taccggatag ggagacccgc accatggtga gcaagcagat cctgaagaac accggcctgc
     6361 aggagatcat gagcttcaag gtgaacctgg agggcgtggt gaacaaccac gtgttcacca
     6421 tggagggctg cggcaagggc aacatcctgt tcggcaacca gctggtgcag atccgcgtga
     6481 ccaagggcgc ccccctgccc ttcgccttcg acatcctgag ccccgccttc cagtacggca
     6541 accgcacctt caccaagtac cccgaggaca tcagcgactt cttcatccag agcttccccg
     6601 ccggcttcgt gtacgagcgc accctgcgct acgaggacgg cggcctggtg gagatccgca
     6661 gcgacatcaa cctgatcgag gggatgttcg tgtaccgcgt ggagtacaag ggccgcaact
     6721 tccccaacga cggccccgtg atgaagaaga ccatcaccgg cctgcagccc agcttcgagg
     6781 tggtgtacat gaacgacggc gtgctggtgg gccaggtgat cctggtgtac cgcctgaaca
     6841 gcggcaagtt ctacagctgc cacatgcgca ccctgatgaa gagcaagggc gtggtgaagg
     6901 acttccccga gtaccacttc atccagcacc gcctggagaa gacctacgtg gaggacggcg
     6961 gcttcgtaga gcagcacgag accgccatcg cccagctgac cagcctgggc aagcccctgg
     7021 gcagcctgca cgagtgggtg gagctctccg gactcagatc tcgaccgcgg gcccgcgacc
     7081 ctcccgtggg ccccaagaag aagcgcaagg tgggcgccta agatccaccg gatctagata
     7141 atagggtacc acctttagtg agggttaatt gctagagctc gctgatcagc ctcgactgtg
     7201 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     7261 ggtgccactc tcactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     7321 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     7381 gacaatagca ggcatgctgg ggcgccggcg cgttaaggcg taaattgtaa gcgttaattt
     7441 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     7501 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     7561 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     7621 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatctag agtcgcggcc
     7681 gctttacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
     7741 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact ggtagctcag
     7801 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
     7861 gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat
     7921 gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt tgtactccag
     7981 cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt
     8041 caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt
     8101 gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg
     8161 ctgcttcatg tggtcggggt agcgggcgaa gcactgcagg ccgtagccga aggtggtcac
     8221 gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt
     8281 gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc
     8341 gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac
     8401 catggtggcg accggtataa cttcgtataa tgtatgctat acgaagttat gtcgactgca
     8461 gaatttcgag ataacttcgt atagcataca ttatacgaag ttataccggc tagtgattcg
     8521 ccaccatggt ggcctcctcc gaggacgtca tcaaagagtt catgcgcttc aaggtgcgca
     8581 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     8641 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct
     8701 gggacatcct gtccccccag ttccagtacg gctccaaggc gtacgtgaag caccccgccg
     8761 acatccccga ctacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     8821 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcacgc
     8881 tgatctacaa ggtgaagttc cgcggcacca acttcccccc cgacggcccc gtaatgcaga
     8941 agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga
     9001 agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca
     9061 agaccatcta catggccaag aagcccgtgc agctgcccgg ctactactac gtggacacca
     9121 agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgctccg
     9181 agggccgcca ccacctgttc ctggggcatg gcaccggcag caccggcagc ggcagctccg
     9241 gcaccgcctc ctccgaggac gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg
     9301 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     9361 gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca
     9421 tcctgtcccc ccagttccag tacggctcca aggcgtacgt gaagcacccc gccgacatcc
     9481 ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     9541 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc acgctgatct
     9601 acaaggtgaa gttccgcggc accaacttcc cccccgacgg ccccgtaatg cagaagaaga
     9661 ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg
     9721 agatccacca ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagacca
     9781 tctacatggc caagaagccc gtgcagctgc ccggctacta ctacgtggac accaagctgg
     9841 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc tccgagggcc
     9901 gccaccacct gttcctgtag gaattggccg cgactctagg attgatctag agtcgcggct
     9961 agataatagg gtaccacctt tagtgagggt taattgctag agctcgctga tcagcctcga
    10021 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
    10081 tggaaggtgc cactctcact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
    10141 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
    10201 gggaagacaa tagcaggcat gctggggcgc cggcgcgtta aggcgtaaat tgtaagcgtt
    10261 aatttaagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
    10321 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
    10381 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
    10441 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tctagccgct
    10501 ttacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
    10561 gaccatgtga tcgcgcttct cgttggggtc tttgctcagc ttggactggg tgctcaggta
    10621 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
    10681 ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttgg ccttgatgcc
    10741 gttcttctgc ttgtcggcgg tgatatagac gttgtcgctg atggcgttgt actccagctt
    10801 gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac
    10861 cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa
    10921 gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg
    10981 cttcatgtgg tcggggtagc gggcgaagca ctgcacgccc caggtcaggg tggtcacgag
    11041 ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc
    11101 gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc
    11161 gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat
    11221 ggtggcgacc ggtggatccc gggcccgcgg taccgtcgac tgcagctcct gatcggcctc
    11281 ttcattcttc tcaaccggtt tagttcttct gatgcagcac agcatggtgg ctgtgaatta
    11341 attccggtat aacttcgtat aatgtatgct atacgaagtt atctcgaatc gcgcgcgaag
    11401 ttcctattct ctagaaagta taggaacttc tcgaagcttc gaggtccttc ctctgcagag
    11461 gtcttgcttc tcccggtcag ctgactccct ccccaagtcc ttcaaatatc tcagaacatg
    11521 gggagaaacg gggaccttgt ccctcctaag gaaccccagt gctgcatgcc atcatccccc
    11581 ccaccctcgc ccccaccccc gccacttctc cctccatgca taccactagc tgtcattttg
    11641 tactctgtat ttattctagg gctgcttctg attatttagt ttgttctttc cctggagacc
    11701 tgttagaaca taagggcgta tggtgggtag gggaggcagg atatcagtcc cctggggcga
    11761 gttcctccct gccaaccaag ccagatgcct gaaagagata tggatgaggg aagttggact
    11821 gtgcctgtac ctggtacagt catactctgt tgaaagaatc atcggggagg ggggggggct
    11881 caagagggga gagctctctg agcctttgtg gaccatccaa tgaggatgag ggcttagatt
    11941 ctaccaggtc attctcagcc accacacaca agcgctctgc catcactgaa gaagccccct
    12001 agggctcttg ggccagggca cactcagtaa agatgcaggt tcagtcaggg aatgatgggg
    12061 aaaggggtag gaggtggggg agggatcacc ccctcctcta aaacacgagc ctgctgtctc
    12121 caaaggcctc tgcctgtagt gagggtggca gaagaagaca aggagccaga actctgactc
    12181 caggatctaa gtccgtgcag gaaggggatc ctagaaccat ctggttggac ccagcttacc
    12241 aagggagagc ctttattcct tctttccctt gcccctctgt gccagcccct cttgctgtcc
    12301 ctgatccccc agacagcgag agtcttgcaa cctgcctctt ccaagacctc ctaatctcag
    12361 gggcaggcgg tggagtgaga tccggcgtgc acactttttg gaagatagct ttcccaagga
    12421 tcctctcccc cactggcagc tctgcctgtc ccatcaccat gtataatacc accactgcta
    12481 cagcatctca ccgaggaaag aaaactgcac aataaaacca agcctctgga gtgtgtcctg
    12541 gtgtctgtct cttctgtgtc ctggcgtctg tctcttctgt gttcttccaa ggtcagaaac
    12601 aaaaaccaca cacttcaacc tggatggctc ggctgagcac ttctgtgtgc agaaggtcca
    12661 accagactct ggggtacccc ggccctccct attcccttgc ctcctgtctc ccgcttttta
    12721 tagctcccta tgctgggctt ctctggagag tgaaatcttt gcccaaatca atgcgcattc
    12781 tctctgctga gtcatctggc gacagcagtt gagttcaccc gccaacacat gggcccagct
    12841 atgtagccga accctggctc tggaagtgcc agggactttg tgcataagta tgtaccatgc
    12901 ccttttttca cagtcctagc tctgcagaag tgcagcctga aggcctgtct gctgagagga
    12961 catgccctgg agccctgaaa caggcacagt gggaggagga acggaggatg acaggcatca
    13021 ggccctcagt ccaaaagcaa ccacttgaga atgggctgga gtacgaaaca tggggtcccg
    13081 tccctggatc cctcctcaaa gagtaataag taaaatataa acaggtaccc caggccgttc
    13141 tgggtttggg ttgtaatggg atccatttgc agagaactat tgagacagcc cagccgtact
    13201 gtgacaggca atgtggggga ggaggttgaa tcacttggta tttagcatga atagaataat
    13261 tccctgaaca tttttcttaa acatccatat ctaaattacc accactcgct cccagtcttc
    13321 ctgcctttgc gccagcctcc tgtctggcca tgcctgaaga aggctggaga agccacccac
    13381 ctcaggccat gacactgcca gccacttggc aggtgcagcc aaacctgagc tgtcccagaa
    13441 agggacattc tcaagaccca ggcaccctga tcagcactga cttggagcta caagtgtcat
    13501 gccagaaaag tctctaagaa aaccttttca gggaaaaggg ggtgactcaa caccgggcaa
    13561 gtttgggaag ccccaccctt cgagtgatgg aagagcagat aggaagcctc agaagagaga
    13621 caccggcacc caggtaacgt tcctcatgtg gtctctgtca cactaggtgc tcttccctgg
    13681 acatctccgt gaccacactc tcagttctta gggagatgcg ggtgctctct gaggctatct
    13741 cagagttgca gattctgagg cctagagtga ctacagtcag cctaggaagc cacagaggac
    13801 tgtggaccag gagggcagaa gaggagaagg gaagaaaaac catcagatag gacttgcaat
    13861 gaaactaacc caagacaatc ataatgcaga caggaatgtt aaaggcgttc agcagctggc
    13921 catgacaccc atctgtccct ctggccaagt cagcaagcct ggaagacctg ggactcctgc
    13981 ccatatgtcc taagctcccc acccacccac tcgttcactg tccttattct ctctctacct
    14041 tcagccactt agtttcctac cttaagtcct agaattgatc ctggcgtaat agcgaagagg
    14101 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
    14161 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
    14221 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
    14281 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
    14341 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcga
//
