LOCUS       sequence-88470-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: ER, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos-A69V-ER-3
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..52
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     3..23
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             141..818
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFVEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt
       61 gaaccgtcag atccgctagc atgctgctat ccgtgccgtt gctgctcggc ctcctcggcc
      121 tggccgtcgc cgaccggtcg atgagtgcga ttaagccaga catgaagatc aacctccgta
      181 tggaaggcaa cgtaaacggg caccactttg tgatcgacgg agatggtaca ggcaagcctt
      241 ttgagggaaa acagagtatg gatcttgaag tcaaagaggg cggacctctg ccttttgcct
      301 ttgatatcct gaccactgca ttccattacg gcaacagggt attcgtcgaa tatccagacc
      361 acatacaaga ctattttaag cagtcgtttc ctaaggggta ttcgtgggaa cgaagcttga
      421 ctttcgaaga cgggggcatt tgcattgcca gaaacgacat aacaatggaa ggggacactt
      481 tctataataa agttcgattt cacggtacaa actttcccgc caatggtcca gttatgcaga
      541 agaagacgct gaaatgggag ccctccactg agaaaatgta tgtgcgtgat ggagtgctga
      601 cgggtgatat tcacatggct ttgttgcttg aaggaaatgc ccattaccga tgtgacttca
      661 gaactactta caaagctaag gagaagggtg tcaagttacc aggctaccac tttgtggacc
      721 actgcattga gattttaagc catgacaaag attacaacaa ggttaagctg tatgagcatg
      781 ctgttgctca ttctggattg cctgacaatg ccagacgaaa ggacgagctg taagaattct
      841 gcagtcgacg gtaccgcggg cccgggatcc accggatcta gataactgat cataatcagc
      901 cataccacat ttgtagaggt tttacttgct ttaaaaa
//
