LOCUS       sequence-88473-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Contacts, Excitation: 505 / 569, Emission: 516 /
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos-A69V-Paxillin-22
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    82..134
                     /label=MCS
                     /note="multiple cloning site"
     CDS             148..828
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFVEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 ctcaagcagc tcaacaaagg aaccttcaaa gaacagaacg acaagcccta ctgtcagaac
       61 tgctttctca aactcttctg tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg
      121 cgggcccggg atccaccggt cgccaccatg agtgcgatta agccagacat gaagatcaac
      181 ctccgtatgg aaggcaacgt aaacgggcac cactttgtga tcgacggaga tggtacaggc
      241 aagccttttg agggaaaaca gagtatggat cttgaagtca aagagggcgg acctctgcct
      301 tttgcctttg atatcctgac cactgcattc cattacggca acagggtatt cgtcgaatat
      361 ccagaccaca tacaagacta ttttaagcag tcgtttccta aggggtattc gtgggaacga
      421 agcttgactt tcgaagacgg gggcatttgc attgccagaa acgacataac aatggaaggg
      481 gacactttct ataataaagt tcgatttcac ggtacaaact ttcccgccaa tggtccagtt
      541 atgcagaaga agacgctgaa atgggagccc tccactgaga aaatgtatgt gcgtgatgga
      601 gtgctgacgg gtgatattca catggctttg ttgcttgaag gaaatgccca ttaccgatgt
      661 gacttcagaa ctacttacaa agctaaggag aagggtgtca agttaccagg ctaccacttt
      721 gtggaccact gcattgagat tttaagccat gacaaagatt acaacaaggt taagctgtat
      781 gagcatgctg ttgctcattc tggattgcct gacaatgcca gacgataagc ggccgcgact
      841 ctagatcata atcagccata ccacat
//
