LOCUS       sequence-88475-e        1939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: mEos2-Actin-C-18
FEATURES             Location/Qualifiers
     source          1..1939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..713
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggtcg ccaccatgag tgcgattaag ccagacatga
       61 agatcaaact ccgtatggaa ggcaacgtaa acgggcacca ctttgtgatc gacggagatg
      121 gtacaggcaa gccttttgag ggaaaacaga gtatggatct tgaagtcaaa gagggcggac
      181 ctctgccttt tgcctttgat atcctgacca ctgcattcca ttacggcaac agggtattcg
      241 ccaaatatcc agacaacata caagactatt ttaagcagtc gtttcctaag gggtattcgt
      301 gggaacgaag cttgactttc gaagacgggg gcatttgcat tgccagaaac gacataacaa
      361 tggaagggga cactttctat aataaagttc gattttatgg taccaacttt cccgccaatg
      421 gtccagttat gcagaagaag acgctgaaat gggagccctc cactgagaaa atgtatgtgc
      481 gtgatggagt gctgacgggt gatattcata tggctttgtt gcttgaagga aatgcccatt
      541 accgatgtga cttcagaact acttacaaag ctaaggagaa gggtgtcaag ttaccaggct
      601 accactttgt ggaccactgc attgagattt taagccatga caaagattac aacaaggtta
      661 agctgtatga gcatgctgtt gctcattctg gattgcctga caatgccaga cgatccggac
      721 tcagatctgg cagcggtgga ggcagcgcat ccggcggaag cggaagcgat gatgatatcg
      781 ccgcgctcgt cgtcgacaac ggctccggca tgtgcaaggc cggcttcgcg ggcgacgatg
      841 ccccccgggc cgtcttcccc tccatcgtgg ggcgccccag gcaccagggc gtgatggtgg
      901 gcatgggtca gaaggattcc tatgtgggcg acgaggccca gagcaagaga ggcatcctca
      961 ccctgaagta ccccatcgag cacggcatcg tcaccaactg ggacgacatg gagaaaatct
     1021 ggcaccacac cttctacaat gagctgcgtg tggctcccga ggagcacccc gtgctgctga
     1081 ccgaggcccc cctgaacccc aaggccaacc gcgagaagat gacccagatc atgtttgaga
     1141 ccttcaacac cccagccatg tacgttgcta tccaggctgt gctatccctg tacgcctctg
     1201 gccgtaccac tggcatcgtg atggactccg gtgacggggt cacccacact gtgcccatct
     1261 acgaggggta tgccctcccc catgccatcc tgcgtctgga cctggctggc cgggacctga
     1321 ctgactacct catgaagatc ctcaccgagc gcggctacag cttcaccacc acggccgagc
     1381 gggaaatcgt gcgtgacatt aaggagaagc tgtgctacgt cgccctggac ttcgagcaag
     1441 agatggccac ggctgcttcc agctcctccc tggagaagag ctacgagctg cctgacggcc
     1501 aggtcatcac cattggcaat gagcggttcc gctgccctga ggcactcttc cagccttcct
     1561 tcctgggcat ggagtcctgt ggcatccacg aaactacctt caactccatc atgaagtgtg
     1621 acgtggacat ccgcaaagac ctgtacgcca acacagtgct gtctggcggc accaccatgt
     1681 accctggcat tgccgacagg atgcagaagg agatcactgc cctggcaccc agcacaatga
     1741 agatcaagat cattgctcct cctgagcgca agtactccgt gtggatcggc ggctccatcc
     1801 tggcctcgct gtccaccttc cagcagatgt ggatcagcaa gcaggagtat gacgagtccg
     1861 gcccctccat cgtccaccgc aaatgcttct agggatccac cggatctaga taactgatca
     1921 taatcagcca taccacatt
//
