LOCUS       sequence-88481-e        4007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Jaqaman K, Galbraith JA, Davidson MW, Galbraith CG
  TITLE     Changes in single-molecule integrin dynamics linked to local 
            cellular behavior.
  JOURNAL   Mol Biol Cell. 2016 May 15;27(10):1561-9. doi: 
            10.1091/mbc.E16-01-0018. Epub 2016 Mar 23.
  PUBMED    27009207
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E16-01-0018"; journalName: "Mol Biol Cell"; date: 
            "2016-05-15- 15"; volume: "27"; issue: "10"; pages: "1561-9"
COMMENT     Sequence Label: mEos2-Alpha-V-Integrin-25
FEATURES             Location/Qualifiers
     source          1..4007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    3186..3238
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      3248..3257
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3254..3964
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3399..3417)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3667..3686
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gacgtcagat ccgctagtcg acggtacctc ggcgatggct tttccgccgc ggcgacggct
       61 gcgcctcggt ccccgcggcc tcccgcttct tctctcggga ctcctgctac ctctgtgccg
      121 cgccttcaac ctagacgtgg acagtcctgc cgagtactct ggccccgagg gaagttactt
      181 cggcttcgcc gtggatttct tcgtgcccag cgcgtcttcc cggatgtttc ttctcgtggg
      241 agctcccaaa gcaaacacca cccagcctgg gattgtggaa ggagggcagg tcctcaaatg
      301 tgactggtct tctacccgcc ggtgccagcc aattgaattt gatgcaacag gcaatagaga
      361 ttatgccaag gatgatccat tggaatttaa gtcccatcag tggtttggag catctgtgag
      421 gtcgaaacag gataaaattt tggcctgtgc cccattgtac cattggagaa ctgagatgaa
      481 acaggagcga gagcctgttg gaacatgctt tcttcaagat ggaacaaaga ctgttgagta
      541 tgctccatgt agatcacaag atattgatgc tgatggacag ggattttgtc aaggaggatt
      601 cagcattgat tttactaaag ctgacagagt acttcttggt ggtcctggta gcttttattg
      661 gcaaggtcag cttatttcgg atcaagtggc agaaatcgta tctaaatacg accccaatgt
      721 ttacagcatc aagtataata accaattagc aactcggact gcacaagcta tttttgatga
      781 cagctatttg ggttattctg tggctgtcgg agatttcaat ggtgatggca tagatgactt
      841 tgtttcagga gttccaagag cagcaaggac tttgggaatg gtttatattt atgatgggaa
      901 gaacatgtcc tccttataca attttactgg cgagcagatg gctgcatatt tcggattttc
      961 tgtagctgcc actgacatta atggagatga ttatgcagat gtgtttattg gagcacctct
     1021 cttcatggat cgtggctctg atggcaaact ccaagaggtg gggcaggtct cagtgtctct
     1081 acagagagct tcaggagact tccagacgac aaagctgaat ggatttgagg tctttgcacg
     1141 gtttggcagt gccatagctc ctttgggaga tctggaccag gatggtttca atgatattgc
     1201 aattgctgct ccatatgggg gtgaagataa aaaaggaatt gtttatatct tcaatggaag
     1261 atcaacaggc ttgaacgcag tcccatctca aatccttgaa gggcagtggg ctgctcgaag
     1321 catgccacca agctttggct attcaatgaa aggagccaca gatatagaca aaaatggata
     1381 tccagactta attgtaggag cttttggtgt agatcgagct atcttataca gggccagacc
     1441 agttatcact gtaaatgctg gtcttgaagt gtaccctagc attttaaatc aagacaataa
     1501 aacctgctca ctgcctggaa cagctctcaa agtttcctgt tttaatgtta ggttctgctt
     1561 aaaggcagat ggcaaaggag tacttcccag gaaacttaat ttccaggtgg aacttctttt
     1621 ggataaactc aagcaaaagg gagcaattcg acgagcactg tttctctaca gcaggtcccc
     1681 aagtcactcc aagaacatga ctatttcaag ggggggactg atgcagtgtg aggaattgat
     1741 agcgtatctg cgggatgaat ctgaatttag agacaaactc actccaatta ctatttttat
     1801 ggaatatcgg ttggattata gaacagctgc tgatacaaca ggcttgcaac ccattcttaa
     1861 ccagttcacg cctgctaaca ttagtcgaca ggctcacatt ctacttgact gtggtgaaga
     1921 caatgtctgt aaacccaagc tggaagtttc tgtagatagt gatcaaaaga agatctatat
     1981 tggggatgac aaccctctga cattgattgt taaggctcag aatcaaggag aaggtgccta
     2041 cgaagctgag ctcatcgttt ccattccact gcaggctgat ttcatcgggg ttgtccgaaa
     2101 caatgaagcc ttagcaagac tttcctgtgc atttaagaca gaaaaccaaa ctcgccaggt
     2161 ggtatgtgac cttggaaacc caatgaaggc tggaactcaa ctcttagctg gtcttcgttt
     2221 cagtgtgcac cagcagtcag agatggatac ttctgtgaaa tttgacttac aaatccaaag
     2281 ctcaaatcta tttgacaaag taagcccagt tgtatctcac aaagttgatc ttgctgtttt
     2341 agctgcagtt gagataagag gagtctcgag tcctgatcat atctttcttc cgattccaaa
     2401 ctgggagcac aaggagaacc ctgagactga agaagatgtt gggccagttg ttcagcacat
     2461 ctatgagctg agaaacaatg gtccaagttc attcagcaag gcaatgctcc atcttcagtg
     2521 gccttacaaa tataataata acactctgtt gtatatcctt cattatgata ttgatggacc
     2581 aatgaactgc acttcagata tggagatcaa ccctttgaga attaagatct catctttgca
     2641 aacaactgaa aagaatgaca cggttgccgg gcaaggtgag cgggaccatc tcatcactaa
     2701 gcgggatctt gccctcagtg aaggagatat tcacactttg ggttgtggag ttgctcagtg
     2761 cttgaagatt gtctgccaag ttgggagatt agacagagga aagagtgcaa tcttgtacgt
     2821 aaagtcatta ctgtggactg agacttttat gaataaagaa aatcagaatc attcctattc
     2881 tctgaagtcg tctgcttcat ttaatgtcat agagtttcct tataagaatc ttccaattga
     2941 ggatatcacc aactccacat tggttaccac taatgtcacc tggggcattc agccagcgcc
     3001 catgcctgtg cctgtgtggg tgatcatttt agcagttcta gcaggattgt tgctactggc
     3061 tgttttggta tttgtaatgt acaggatggg cttttttaaa cgggtccggc cacctcaaga
     3121 agaacaagaa agggagcagc ttcaacctca tgaaaatggt gaaggaaact cagaaactcc
     3181 gggatctcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc cgggatcccc
     3241 accggtcgcc accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     3301 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     3361 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     3421 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     3481 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     3541 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     3601 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     3661 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     3721 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     3781 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     3841 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
     3901 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     3961 gtaaagcggc cgcgactcta gatcataatc agccatacca cattgag
//
