LOCUS       sequence-88487-e        3521 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Chromatin Assembly Factor 1, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: mEos2-CAF1-C-10
FEATURES             Location/Qualifiers
     source          1..3521
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..710
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     protein_bind    2529..2540
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggtcgcca ccatgagtgc gattaagcca gacatgaaga
       61 tcaaactccg tatggaaggc aacgtaaacg ggcaccactt tgtgatcgac ggagatggta
      121 caggcaagcc ttttgaggga aaacagagta tggatcttga agtcaaagag ggcggacctc
      181 tgccttttgc ctttgatatc ctgaccactg cattccatta cggcaacagg gtattcgcca
      241 aatatccaga caacatacaa gactatttta agcagtcgtt tcctaagggg tattcgtggg
      301 aacgaagctt gactttcgaa gacgggggca tttgcattgc cagaaacgac ataacaatgg
      361 aaggggacac tttctataat aaagttcgat tttatggtac caactttccc gccaatggtc
      421 cagttatgca gaagaagacg ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg
      481 atggagtgct gacgggtgat attcatatgg ctttgttgct tgaaggaaat gcccattacc
      541 gatgtgactt cagaactact tacaaagcta aggagaaggg tgtcaagtta ccaggctacc
      601 actttgtgga ccactgcatt gagattttaa gccatgacaa agattacaac aaggttaagc
      661 tgtatgagca tgctgttgct cattctggat tgcctgacaa tgccagacga tccggactca
      721 gatctggcag cggtggaggc ttggaggagc cggaggccgc gacgcggaca gccgcggccg
      781 tggattgcaa agacagaccc gggttcccag tgaagaggtt aatacaagcc cgcttgccct
      841 tcaaacgcct gaaccttgtt cccaaggaga aagttgagga agacacatca ccaaaagcag
      901 ctgtggaaag caaagtcccg gatctacaat tgtctttggg cacttttgaa agccagtgtc
      961 acacaggctc tcacgtaggc ttgagtacaa agttggtcgg tggccagggc cccattgata
     1021 gcttcttgag ggcaacaatc aagccagtac cgagtgtggt cattatcgac ctgacggaga
     1081 actgcagtga cattccagac agccctgagg gccacagtga actaagtcct gacactgctg
     1141 gtgtggtcac cacagtggaa ggagctgcca agcagcagga gcactctgca gcagagctgt
     1201 gtctcctaga gaccccttca gacatcactt gtcacatgga agaggagcct gggagtccag
     1261 gagacccaaa gaggacgggt gactgtcagg ctggttcact acagagctgt ccggagctga
     1321 ccccaggttc taggacatgc cccaccaagg agttatcaag ctggagcaag gcaggggatc
     1381 tcctatttat agagaaagtg cctgtggtgg tgctagagga catcctggcc actaagccct
     1441 ccattgcctc tcttcccatg atgtccctgg ataggagtgt cacttcagag agtgaaatac
     1501 tggagtcctg ccccgaggat gactccatac tgagccactc ctccaccaac tcctcttctc
     1561 caaccagctc tcctgaggga ccatccacac ccccagaaca tcggggtggg agaagctccc
     1621 cctccacccc agcctgcaga gttgccaaga actttgtcaa aggctccaca gagaagggca
     1681 ggagcaaact gcacagagat agagaacagc agagggaaga gaaggaaaag ctgagggaag
     1741 agatacgacg agccaaggag gaggcacgga agaagaagga ggaggagaag gagctgaagg
     1801 aaaaggagcg gagggaaaag cgggagaaag atgagaagga gaaggcggag aagcagcggc
     1861 taaaggagga aaaacgcaag gagcggcagg aagctctgga ggctaaactg gaggagaaga
     1921 ggaagaagga ggaagagaag cggttacggg aggaagagaa gcggttacgg gaagaagaga
     1981 agcgcattaa ggcagaaaag gcagagatca ctcgattctt ccagaaaccg aagactccgc
     2041 aggctcccaa gaccctggcc ggttcctgtg gtaagtttgc cccctttgaa atcaaagaac
     2101 acatggtgct ggctccacgg tgccgagctg ccttggatca agacctgtgc gatcagctgg
     2161 accagctcct gcagcagcag agtgtggcaa gcaccttcct cagtgaccta aaaagccggc
     2221 tgcccttgag atcggggccc acccgggtct gtggccatga cacagacatc atgaacaggg
     2281 atgtggtcat tgtggagagc agcaaggtgg acggtgtgtc tgaaaggaag aagtttggcc
     2341 gcatgaagct tctgcagttc tcagagaacc accggccagc atactggggg acgtggaaca
     2401 agaagacagc catcatccgc ccccggaacc cctgggccca ggacaaagac cttcttgact
     2461 atgaggtaga cagtgacgat gagtgggaag aggaggaacc tggggagtct ttgtcccaca
     2521 gtgaggggga tgaggatgac gatgtggggg aagatgagga tgaggacgat ggcttctttg
     2581 tgccccatgg gtacttatct gaagatgaag gcgtgactga ggagtgtgca gaccctgaga
     2641 accacaaagt acaccagaag ctgaaggcca aggagtggga tgagctccta gccaagggca
     2701 agcgctttcg tgtgctgcag cctgtgcacg tgggatgtgt atgggcatca gaggcagcca
     2761 actgcacgag ttccgacttg aagctgctgc agcagttcac tgcatgcctg ctggatgtgg
     2821 catcccctga tgagccagaa cccggggcct ccaggaggga aaagagggac cagcatatcc
     2881 tggcccagtt gcttccgctg ctacatggca atgtgaatgg gagcaaggtg atcatccatg
     2941 aattccagga gcaatgccgc aggggcctgc tgacccttcc aagtcccacc ccacacctgc
     3001 agatgcccaa cttggaggat gctgttgctg tgccatccaa ggccaggctg aagcgcctta
     3061 tctctgagaa ctctgcctat gagaagcggc ccaacttccg tatgtgctgg tatgtgcacc
     3121 cagaggtgct caagagcttt ggccaggagt gcctgccagt gccctgccag tggacctaca
     3181 tcaccaccat gccctcagca cccagggaag acagtggcag tgcttctact gaggggcctg
     3241 gccagagcac acctatgcta ctcaagcgga agccagcggc caccatgtgc atcacgcagt
     3301 ttatgaagaa gcggcgctat gatggacagg ttgggtctgg ggacatggat ggcttccagg
     3361 cagacacaga agaggatgaa gaggatgaca cagactgtat gatcatagat gtgccagatg
     3421 ttgggagtga cgtgtcagaa gctcccatac ctgctcccac actttgcaag tgaggatcca
     3481 ccggatctag ataactgatc ataatcagcc ataccacatt t
//
