LOCUS       sequence-88493-e        2389 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: mEos2-CD36-C-10
FEATURES             Location/Qualifiers
     source          1..2389
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        42..141
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     92..112
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             186..863
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 aagtctccac cccattgacg tcaatgggag tttgtttggc accaaaatca acgggacttt
       61 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
      121 gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg
      181 ccaccatgag tgcgattaag ccagacatga agatcaaact ccgtatggaa ggcaacgtaa
      241 acgggcacca ctttgtgatc gacggagatg gtacaggcaa gccttttgag ggaaaacaga
      301 gtatggatct tgaagtcaaa gagggcggac ctctgccttt tgcctttgat atcctgacca
      361 ctgcattcca ttacggcaac agggtattcg ccaaatatcc agacaacata caagactatt
      421 ttaagcagtc gtttcctaag gggtattcgt gggaacgaag cttgactttc gaagacgggg
      481 gcatttgcat tgccagaaac gacataacaa tggaagggga cactttctat aataaagttc
      541 gattttatgg taccaacttt cccgccaatg gtccagttat gcagaagaag acgctgaaat
      601 gggagccctc cactgagaaa atgtatgtgc gtgatggagt gctgacgggt gatattcata
      661 tggctttgtt gcttgaagga aatgcccatt accgatgtga cttcagaact acttacaaag
      721 ctaaggagaa gggtgtcaag ttaccaggct accactttgt ggaccactgc attgagattt
      781 taagccatga caaagattac aacaaggtta agctgtatga gcatgctgtt gctcattctg
      841 gattgcctga caatgccaga cgatccggac tcagatctgg cagcagtgga ggtggctgtg
      901 accggaactg tgggctcatc gctggggctg tcattggtgc tgtcctggct gtgtttggag
      961 gtattctaat gccagttgga gacctgctta tccagaagac aattaaaaag caagttgtcc
     1021 tcgaagaagg tacaattgct tttaaaaatt gggttaaaac aggcacagaa gtttacagac
     1081 agttttggat ctttgatgtg caaaatccac aggaagtgat gatgaacagc agcaacattc
     1141 aagttaagca aagaggtcct tatacgtaca gagttcgttt tctagccaag gaaaatgtaa
     1201 cccaggacgc tgaggacaac acagtctctt tcctgcagcc caatggtgcc atcttcgaac
     1261 cttcactatc agttggaaca gaggctgaca acttcacagt tctcaatctg gctgtggcag
     1321 ctgcatccca tatctatcaa aatcaatttg ttcaaatgat cctcaattca cttattaaca
     1381 agtcaaaatc ttctatgttc caagtcagaa ctttgagaga actgttatgg ggctataggg
     1441 atccattttt gagtttggtt ccgtaccctg ttactaccac agttggtctg ttttatcctt
     1501 acaacaatac tgcagatgga gtttataaag ttttcaatgg aaaagataac ataagtaaag
     1561 ttgccataat cgacacatat aaaggtaaaa ggaatctgtc ctattgggaa agtcactgcg
     1621 acatgattaa tggtacagat gcagcctcat ttccaccttt tgttgagaaa agccaggtat
     1681 tgcagttctt ttcttctgat atttgcaggt caatctatgc tgtatttgaa tccgacgtta
     1741 atctgaaagg aatccctgtg tatagatttg ttcttccatc caaggccttt gcctctccag
     1801 ttgaaaaccc agacaactat tgtttctgca cagaaaaaat tatctcaaaa aattgtacat
     1861 catatggtgt gctagacatc agcaaatgca aagaagggag acctgtgtac atttcacttc
     1921 ctcattttct gtatgcaagt cctgatgttt cagaacctat tgatggatta aacccaaatg
     1981 aagaagaaca taggacatac ttggatattg aacctataac tggattcact ttacaatttg
     2041 caaaacggct gcaggtcaac ctattggtca agccatcaga aaaaattcaa gtattaaaga
     2101 atctgaagag gaactatatt gtgcctattc tttggcttaa tgagactggg accattggtg
     2161 atgagaaggc aaacatgttc agaagtcaag taactggaaa aataaacctc cttggcctga
     2221 tagaaatgat cttactcagt gttggtgtgg tgatgtttgt tgcttttatg atttcatatt
     2281 gtgcatgcag atcgaaaaca ataaaataag aattctgcag tcgacggtac cgcgggcccg
     2341 ggatccaccg gatctaagat aactgatcat aatcagccat accacattt
//
