LOCUS       sequence-88495-e        1317 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: mEos2-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..1317
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    532..584
                     /label=MCS
                     /note="multiple cloning site"
     CDS             598..1278
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 tgacggtcag atccgctagc caccatgggc cccaagcggc ggcagctgac gttccgggag
       61 aagtcgcgga tcatccagga ggtggaggag aacccggacc tacgcaaggg cgagatcgcg
      121 cggcgcttca acatcccgcc gtccacgctg agcacgatcc tgaagaacaa gcgcgccatc
      181 ctggcgtcgg agcgcaagta cggggtggcc tccacctgcc gcaagaccaa caagctgtct
      241 ccctacgaca agctcgaggg cttgctcatc gcctggttcc agcagatccg cgccgccggc
      301 ctgccggtca agggcatcat cctcaaggag aaggcgctgc gcatagccga ggagctgggc
      361 atggacgact tcaccgcctc caacggctgg ctggaccgct tccgccggcg ccacggcgtg
      421 gtgtcctgca gcggcgtggc ccgcgcccgc gcgcgaaacg ctgccccccg caccccggcg
      481 gcgcctgcca gtccggccgc ggtgccctcg gagggcagtg gcgggagcac tctcgagctc
      541 aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg atccaccggt cgccaccatg
      601 agtgcgatta agccagacat gaagatcaaa ctccgtatgg aaggcaacgt aaacgggcac
      661 cactttgtga tcgacggaga tggtacaggc aagccttttg agggaaaaca gagtatggat
      721 cttgaagtca aagagggcgg acctctgcct tttgcctttg atatcctgac cactgcattc
      781 cattacggca acagggtatt cgccaaatat ccagacaaca tacaagacta ttttaagcag
      841 tcgtttccta aggggtattc gtgggaacga agcttgactt tcgaagacgg gggcatttgc
      901 attgccagaa acgacataac aatggaaggg gacactttct ataataaagt tcgattttat
      961 ggtaccaact ttcccgccaa tggtccagtt atgcagaaga agacgctgaa atgggagccc
     1021 tccactgaga aaatgtatgt gcgtgatgga gtgctgacgg gtgatattca tatggctttg
     1081 ttgcttgaag gaaatgccca ttaccgatgt gacttcagaa ctacttacaa agctaaggag
     1141 aagggtgtca agttaccagg ctaccacttt gtggaccact gcattgagat tttaagccat
     1201 gacaaagatt acaacaaggt taagctgtat gagcatgctg ttgctcattc tggattgcct
     1261 gacaatgcca gacgataagc ggccgcgact ctagatcata atcagccata ccacatt
//
