LOCUS       sequence-88498-e        2398 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane/Vesicles, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: mEos2-C-Src-7
FEATURES             Location/Qualifiers
     source          1..2398
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..98
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             1677..2357
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattcatgg
       61 ggagcagcaa gagcaagccc aaggacccca gccagcgccg gcgcagcctg gagccacccg
      121 acagcaccca ccacggggga ttcccagcct cgcagacccc caacaagaca gcagcccccg
      181 acacgcaccg cacccccagc cgctcctttg ggaccgtggc caccgagccc aagctcttcg
      241 ggggcttcaa cacttctgac accgtcacgt cgccgcagcg tgccggggca ctggctggcg
      301 gcgtcaccac tttcgtggct ctctatgact acgagtcccg gactgaaacg gacttgtcct
      361 tcaagaaagg agaacgcctg cagattgtca acaacacgga aggtgactgg tggctggctc
      421 attccctcac tacaggacag acgggctaca tccccagtaa ctatgtcgcg ccctcagact
      481 ccatccaggc tgaagagtgg tactttggga agatcactcg tcgggagtcc gagcggctgc
      541 tgctcaaccc cgaaaacccc cggggaacct tcttggtccg ggagagcgag acgacaaaag
      601 gtgcctattg cctctccgtt tctgactttg acaacgccaa ggggctcaat gtgaagcact
      661 acaagatccg caagctggac agcggcggct tctacatcac ctcacgcaca cagttcagca
      721 gcctgcagca gctggtggcc tactactcca aacatgctga tggcttgtgc caccgcctga
      781 ccaacgtctg ccccacgtcc aagccccaga cccagggact cgccaaggac gcgtgggaaa
      841 tcccccggga gtcgctgcgg ctggaggtga agctggggca gggctgcttt ggagaggtct
      901 ggatggggac ctggaacggc accaccagag tggccataaa gactctgaag cccggcacca
      961 tgtccccgga ggccttcctg caggaagccc aagtgatgaa gaagctccgg catgagaagc
     1021 tggttcagct gtacgcagtg gtgtcggaag agcccatcta catcgtcact gagtacatga
     1081 gcaaggggag cctcctggat ttcctgaagg gagagatggg caagtacctg cggctgccac
     1141 agctcgtcga tatggctgct cagattgcat ccggcatggc ctatgtggag aggatgaact
     1201 acgtgcaccg agacctgcgg gcggccaaca tcctggtggg ggagaacctg gtgtgcaagg
     1261 tggctgactt tgggctggca cgcctcatcg aggacaacga gtacacagca cggcaaggtg
     1321 ccaagttccc catcaagtgg acagcccccg aggcagccct ctatggccgg ttcaccatca
     1381 agtcggatgt ctggtccttc ggcatcctgc tgactgagct gaccaccaag ggccgggtgc
     1441 catacccagg gatggtcaac agggaggtgc tggaccaggt ggagaggggc taccgcatgc
     1501 cctgcccgcc cgagtgcccc gagtcgctgc atgacctcat gtgccagtgc tggcggaagg
     1561 accctgagga gcggcccact tttgagtacc tgcaggcctt cctggaggac tacttcacct
     1621 cgacagagcc ccagtaccag cctggagaga acctacggga tccaccggtc gccaccatga
     1681 gtgcgattaa gccagacatg aagatcaaac tccgtatgga aggcaacgta aacgggcacc
     1741 actttgtgat cgacggagat ggtacaggca agccttttga gggaaaacag agtatggatc
     1801 ttgaagtcaa agagggcgga cctctgcctt ttgcctttga tatcctgacc actgcattcc
     1861 attacggcaa cagggtattc gccaaatatc cagacaacat acaagactat tttaagcagt
     1921 cgtttcctaa ggggtattcg tgggaacgaa gcttgacttt cgaagacggg ggcatttgca
     1981 ttgccagaaa cgacataaca atggaagggg acactttcta taataaagtt cgattttatg
     2041 gtaccaactt tcccgccaat ggtccagtta tgcagaagaa gacgctgaaa tgggagccct
     2101 ccactgagaa aatgtatgtg cgtgatggag tgctgacggg tgatattcat atggctttgt
     2161 tgcttgaagg aaatgcccat taccgatgtg acttcagaac tacttacaaa gctaaggaga
     2221 agggtgtcaa gttaccaggc taccactttg tggaccactg cattgagatt ttaagccatg
     2281 acaaagatta caacaaggtt aagctgtatg agcatgctgt tgctcattct ggattgcctg
     2341 acaatgccag acgataagcg gccgcgactc tagatcataa tcagccatac cacatgag
//
