LOCUS       sequence-88507-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: mEos2-ER-5
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        28..79
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     30..50
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             180..857
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
       61 ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgcc accatgctgc
      121 tatccgtgcc gttgctgctc ggcctcctcg gcctggccgt cgccgcaccg gtcgccacca
      181 tgagtgcgat taagccagac atgaagatca aactccgtat ggaaggcaac gtaaacgggc
      241 accactttgt gatcgacgga gatggtacag gcaagccttt tgagggaaaa cagagtatgg
      301 atcttgaagt caaagagggc ggacctctgc cttttgcctt tgatatcctg accactgcat
      361 tccattacgg caacagggta ttcgccaaat atccagacaa catacaagac tattttaagc
      421 agtcgtttcc taaggggtat tcgtgggaac gaagcttgac tttcgaagac gggggcattt
      481 gcattgccag aaacgacata acaatggaag gggacacttt ctataataaa gttcgatttt
      541 atggtaccaa ctttcccgcc aatggtccag ttatgcagaa gaagacgctg aaatgggagc
      601 cctccactga gaaaatgtat gtgcgtgatg gagtgctgac gggtgatatt catatggctt
      661 tgttgcttga aggaaatgcc cattaccgat gtgacttcag aactacttac aaagctaagg
      721 agaagggtgt caagttacca ggctaccact ttgtggacca ctgcattgag attttaagcc
      781 atgacaaaga ttacaacaag gttaagctgt atgagcatgc tgttgctcat tctggattgc
      841 ctgacaatgc cagacgatcc ggaaaggacg agctgtaaga attctgcagt cgacggtacc
      901 gcgggcccgg gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta
      961 gaggttttac ttgctttaaa aaacctccca cacc
//
