LOCUS       sequence-88512-e        8758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8758)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 8758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: mEos2-FilaminA-C-14
FEATURES             Location/Qualifiers
     source          1..8758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..714
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gtgacgtcag atccgctagc gctaccggtc gccaccatga gtgcgattaa gccagacatg
       61 aagatcaaac tccgtatgga aggcaacgta aacgggcacc actttgtgat cgacggagat
      121 ggtacaggca agccttttga gggaaaacag agtatggatc ttgaagtcaa agagggcgga
      181 cctctgcctt ttgcctttga tatcctgacc actgcattcc attacggcaa cagggtattc
      241 gccaaatatc cagacaacat acaagactat tttaagcagt cgtttcctaa ggggtattcg
      301 tgggaacgaa gcttgacttt cgaagacggg ggcatttgca ttgccagaaa cgacataaca
      361 atggaagggg acactttcta taataaagtt cgattttatg gtaccaactt tcccgccaat
      421 ggtccagtta tgcagaagaa gacgctgaaa tgggagccct ccactgagaa aatgtatgtg
      481 cgtgatggag tgctgacggg tgatattcat atggctttgt tgcttgaagg aaatgcccat
      541 taccgatgtg acttcagaac tacttacaaa gctaaggaga agggtgtcaa gttaccaggc
      601 taccactttg tggaccactg cattgagatt ttaagccatg acaaagatta caacaaggtt
      661 aagctgtatg agcatgctgt tgctcattct ggattgcctg acaatgccag acgatccgga
      721 gggagcgggg ctggagggag cgggggtggc agcggtagta gctcccactc tcgggcgggc
      781 cagagcgcag caggcgcggc tccgggcggc ggcgtcgaca cgcgggacgc cgagatgccg
      841 gccaccgaga aggacctggc ggaggacgcg ccatggaaga agatccagca gaacactttc
      901 acgcgctggt gcaacgagca cctgaagtgc gtgagcaagc gcatcgccaa cctgcagacg
      961 gacctgagcg acgggctgcg gcttatcgcg ctgttggagg tgctcagcca gaagaagatg
     1021 caccgcaagc acaaccagcg gcccactttc cgccaaatgc agcttgagaa cgtgtcggtg
     1081 gcactcgagt tcctggaccg cgagagcatc aaactggtgt ccatcgacag caaggccatc
     1141 gtggacggga acctgaagct gatcctgggc ctcatctgga ccctgatcct gcactactcc
     1201 atctccatgc ccatgtggga cgaggaggag gatgaggagg ccaagaagca gacccccaag
     1261 cagaggctcc tgggctggat ccagaacaag ctgccgcagc tgcccatcac caacttcagc
     1321 cgggactggc agagcggccg ggccctgggc gccctggtgg acagctgtgc cccgggcctg
     1381 tgtcctgact gggactcttg ggacgccagc aagcccgtta ccaatgcgcg agaggccatg
     1441 cagcaggcgg atgactggct gggcatcccc caggtgatca cccccgagga gattgtggac
     1501 cccaacgtgg acgagcactc tgtcatgacc tacctgtccc agttccccaa ggccaagctg
     1561 aagccagggg ctcccttgcg gcccaaactg aacccgaaga aagcccgtgc ctacgggcca
     1621 ggcatcgagc ccacaggcaa catggtgaag aagcgggcag agttcactgt ggagaccaga
     1681 agtgctggcc agggagaggt gctggtgtac gtggaggacc cggccggaca ccaggaggag
     1741 gcaaaagtga ccgccaataa cgacaagaac cgcaccttct ccgtctggta cgtccccgag
     1801 gtgacgggga ctcataaggt tactgtgctc tttgctggcc agcacatcgc caagagcccc
     1861 ttcgaggtgt acgtggataa gtcacagggt gacgccagca aagtgacagc ccaaggtccc
     1921 ggcctggagc ccagtggcaa catcgccaac aagaccacct actttgagat ctttacggca
     1981 ggagctggca cgggcgaggt cgaggttgtg atccaggacc ccatgggaca gaagggcacg
     2041 gtagagcctc agctggaggc ccggggcgac agcacatacc gctgcagcta ccagcccacc
     2101 atggagggcg tccacaccgt gcacgtcacg tttgccggcg tgcccatccc tcgcagcccc
     2161 tacactgtca ctgttggcca agcctgtaac ccgagtgcct gccgggcggt tggccggggc
     2221 ctccagccca agggtgtgcg ggtgaaggag acagctgact tcaaggtgta cacaaagggc
     2281 gctggcagtg gggagctgaa ggtcaccgtg aagggcccca agggagagga gcgcgtgaag
     2341 cagaaggacc tgggggatgg cgtgtatggc ttcgagtatt accccatggt ccctggaacc
     2401 tatatcgtca ccatcacgtg gggtggtcag aacatcgggc gcagtccctt cgaagtgaag
     2461 gtgggcaccg agtgtggcaa tcagaaggta cgggcctggg gccctgggct ggagggcggc
     2521 gtcgttggca agtcagcaga ctttgtggtg gaggctatcg gggacgacgt gggcacgctg
     2581 ggcttctcgg tggaagggcc atcgcaggct aagatcgaat gtgacgacaa gggcgacggc
     2641 tcctgtgatg tgcgctactg gccgcaggag gctggcgagt atgccgttca cgtgctgtgc
     2701 aacagcgaag acatccgcct cagccccttc atggctgaca tccgtgacgc gccccaggac
     2761 ttccacccag acagggtgaa ggcacgtggg cctggattgg agaagacagg tgtggccgtc
     2821 aacaagccag cagagttcac agtggatgcc aagcacggtg gcaaggcccc acttcgggtc
     2881 caagtccagg acaatgaagg ctgccctgtg gaggcgttgg tcaaggacaa cggcaatggc
     2941 acttacagct gctcctacgt gcccaggaag ccggtgaagc acacagccat ggtgtcctgg
     3001 ggaggcgtca gcatccccaa cagccccttc agggtgaatg tgggagctgg cagccacccc
     3061 aacaaggtca aagtatacgg ccccggagta gccaagacag ggctcaaggc ccacgagccc
     3121 acctacttca ctgtggactg cgccgaggct ggccaggggg acgtcagcat cggcatcaag
     3181 tgtgcccctg gagtggtagg ccccgccgaa gctgacatcg acttcgacat catccgcaat
     3241 gacaatgaca ccttcacggt caagtacacg ccccgggggg ctggcagcta caccattatg
     3301 gtcctctttg ctgaccaggc cacgcccacc agccccatcc gagtcaaggt ggagccctct
     3361 catgacgcca gtaaggtgaa ggccgagggc cctggcctca gtcgcactgg tgtcgagctt
     3421 ggcaagccca cccacttcac agtaaatgcc aaagctgctg gcaaaggcaa gctggacgtc
     3481 cagttctcag gactcaccaa gggggatgca gtgcgagatg tggacatcat cgaccaccat
     3541 gacaacacct acacagtcaa gtacacgcct gtccagcagg gtccagtagg cgtcaatgtc
     3601 acttatggag gggatcccat ccctaagagc cctttctcag tggcagtatc tccaagcctg
     3661 gacctcagca agatcaaggt gtctggcctg ggagagaagg tggacgttgg caaagaccag
     3721 gagttcacag tcaaatcaaa gggtgctggt ggtcaaggca aagtggcatc caagattgtg
     3781 ggcccctcgg gtgcagcggt gccctgcaag gtggagccag gcctgggggc tgacaacagt
     3841 gtggtgcgct tcctgccccg tgaggaaggg ccctatgagg tggaggtgac ctatgacggc
     3901 gtgcccgtgc ctggcagccc ctttcctctg gaagctgtgg cccccaccaa gcctagcaag
     3961 gtgaaggcgt ttgggccggg gctgcaggga ggcagtgcgg gctcccccgc ccgcttcacc
     4021 atcgacacca agggcgccgg cacaggtggc ctgggcctga cggtggaggg cccctgtgag
     4081 gcgcagctcg agtgcttgga caatggggat ggcacatgtt ccgtgtccta cgtgcccacc
     4141 gagcccgggg actacaacat caacatcctc ttcgctgaca cccacatccc tggctcccca
     4201 ttcaaggccc acgtggttcc ctgctttgac gcatccaaag tcaagtgctc aggccccggg
     4261 ctggagcggg ccaccgctgg ggaggtgggc caattccaag tggactgctc gagcgcgggc
     4321 agcgcggagc tgaccattga gatctgctcg gaggcggggc ttccggccga ggtgtacatc
     4381 caggaccacg gtgatggcac gcacaccatt acctacattc ccctctgccc cggggcctac
     4441 accgtcacca tcaagtacgg cggccagccc gtgcccaact tccccagcaa gctgcaggtg
     4501 gaacctgcgg tggacacttc cggtgtccag tgctatgggc ctggtattga gggccagggt
     4561 gtcttccgtg aggccaccac tgagttcagt gtggacgccc gggctctgac acagaccgga
     4621 gggccgcacg tcaaggcccg tgtggccaac ccctcaggca acctgacgga gacctacgtt
     4681 caggaccgtg gcgatggcat gtacaaagtg gagtacacgc cttacgagga gggactgcac
     4741 tccgtggacg tgacctatga cggcagtccc gtgcccagca gccccttcca ggtgcccgtg
     4801 accgagggct gcgacccctc ccgggtgcgt gtccacgggc caggcatcca aagtggcacc
     4861 accaacaagc ccaacaagtt cactgtggag accaggggag ctggcacggg cggcctgggc
     4921 ctggctgtag agggcccctc cgaggccaag atgtcctgca tggataacaa ggacggcagc
     4981 tgctcggtcg agtacatccc ttatgaggct ggcacctaca gcctcaacgt cacctatggt
     5041 ggccatcaag tgccaggcag tcctttcaag gtccctgtgc atgatgtgac agatgcgtcc
     5101 aaggtcaagt gctctgggcc cggcctgagc ccaggcatgg ttcgtgccaa cctccctcag
     5161 tccttccagg tggacacaag caaggctggt gtggccccat tgcaggtcaa agtgcaaggg
     5221 cccaaaggcc tggtggagcc agtggacgtg gtagacaacg ctgatggcac ccagaccgtc
     5281 aattatgtgc ccagccgaga agggccctac agcatctcag tactgtatgg agatgaagag
     5341 gtaccccgga gccccttcaa ggtcaaggtg ctgcctactc atgatgccag caaggtgaag
     5401 gccagtggcc ccgggctcaa caccactggc gtgcctgcca gcctgcccgt ggagttcacc
     5461 atcgatgcaa aggacgccgg ggagggcctg ctggctgtcc agatcacgga tcccgaaggc
     5521 aagccgaaga agacacacat ccaagacaac catgacggca cgtatacagt ggcctacgtg
     5581 ccagacgtga caggtcgcta caccatcctc atcaagtacg gtggtgacga gatccccttc
     5641 tccccgtacc gcgtgcgtgc cgtgcccacc ggggacgcca gcaagtgcac tgtcacaggt
     5701 gctggcatcg gccccaccat tcagattggg gaggagacgg tgatcactgt ggacactaag
     5761 gcggcaggca aaggcaaagt gacgtgcacc gtgtgcacgc ctgatggctc agaggtggat
     5821 gtggacgtgg tggagaatga ggacggcact ttcgacatct tctacacggc cccccagccg
     5881 ggcaaatacg tcatctgtgt gcgctttggt ggcgagcacg tgcccaacag ccccttccaa
     5941 gtgacggctc tggctgggga ccagccctcg gtgcagcccc ctctacggtc tcagcagctg
     6001 gccccacagt acacctacgc ccagggcggc cagcagactt gggccccgga gaggcccctg
     6061 gtgggtgtca atgggctgga tgtgaccagc ctgaggccct ttgaccttgt catccccttc
     6121 accatcaaga agggcgagat cacaggggag gttcggatgc cctcaggcaa ggtggcgcag
     6181 cccaccatca ctgacaacaa agacggcacc gtgaccgtgc ggtatgcacc cagcgaggct
     6241 ggcctgcacg agatggacat ccgctatgac aacatgcaca tcccaggaag ccccttgcag
     6301 ttctatgtgg attacgtcaa ctgtggccat gtcactgcct atgggcctgg cctcacccat
     6361 ggagtagtga acaagcctgc caccttcacc gtcaacacca aggatgcagg agaggggggc
     6421 ctgtctctgg ccattgaggg cccgtccaaa gcagaaatca gctgcactga caaccaggat
     6481 gggacatgca gcgtgtccta cctgcctgtg ctgccggggg actacagcat tctagtcaag
     6541 tacaatgaac agcacgtccc aggcagcccc ttcactgccc gggtcacagg tgacgactcc
     6601 atgcgtatgt cccacctaaa ggtcggctct gctgccgaca tccccatcaa catctcagag
     6661 acggatctca gcctgctgac ggccactgtg gtcccgccct cgggccggga ggagccctgt
     6721 ttgctgaagc ggctgcgtaa tggccacgtg gggatttcat tcgtgcccaa ggagacgggg
     6781 gagcacctgg tgcatgtgaa gaaaaatggc cagcacgtgg ccagcagccc catcccggtg
     6841 gtgatcagcc agtcggaaat tggggatgcc agtcgtgttc gggtctctgg tcagggcctt
     6901 cacgaaggcc acacctttga gcctgcagag tttatcattg atacccgcga tgcaggctat
     6961 ggtgggctca gcctgtccat tgagggcccc agcaaggtgg acatcaacac agaggacctg
     7021 gaggacggga cgtgcagggt cacctactgc cccacagagc caggcaacta catcatcaac
     7081 atcaagtttg ccgaccagca cgtgcctggc agccccttct ctgtgaaggt gacaggcgag
     7141 ggccgggtga aagagagcat cacccgcagg cgtcgggctc cttcagtggc caacgttggt
     7201 agtcattgtg acctcagcct gaaaatccct gaaattagca tccaggatat gacagcccag
     7261 gtgaccagcc catcgggcaa gacccatgag gccgagatcg tggaagggga gaaccacacc
     7321 tactgcatcc gctttgttcc cgctgagatg ggcacacaca cagtcagcgt caagtacaag
     7381 ggccagcacg tgcctgggag ccccttccag ttcaccgtgg ggcccctagg ggaaggggga
     7441 gcccacaagg tccgagctgg gggccctggc ctggagagag ctgaagctgg agtgccagcc
     7501 gaattcagta tctggacccg ggaagctggt gctggaggcc tggccattgc tgtcgagggc
     7561 cccagcaagg ctgagatctc ttttgaggac cgcaaggacg gctcctgtgg tgtggcttat
     7621 gtggtccagg agccaggtga ctacgaagtc tcagtcaagt tcaacgagga acacattccc
     7681 gacagcccct tcgtggtgcc tgtggcttct ccgtctggcg acgcccgccg cctcactgtt
     7741 tctagccttc aggagtcagg gctaaaggtc aaccagccag cctcttttgc agtcagcctg
     7801 aacggggcca agggggcgat cgatgccaag gtgcacagcc cctcaggagc cctggaggag
     7861 tgctatgtca cagaaattga ccaagataag tatgctgtgc gcttcatccc tcgggagaat
     7921 ggcgtttacc tgattgacgt caagttcaac ggtacccaca tccctggaag ccccttcaag
     7981 atccgagttg gggagcctgg gcatggaggg gacccaggct tggtgtctgc ttacggagca
     8041 ggtctggaag gcggtgtcac agggaaccca gctgagttcg tcgtgaacac gagcaatgcg
     8101 ggagctggtg ccctgtcggt gaccattgac ggcccctcca aggtgaagat ggattgccag
     8161 gagtgccctg agggctaccg cgtcacctat acccccatgg cacctggcag ctacctcatc
     8221 tccatcaagt acggcggccc ctaccacatt gggggcagcc ccttcaaggc caaagtcaca
     8281 ggcccccgtc tcgtcagcaa ccacagcctc cacgagacat catcagtgtt tgtagactct
     8341 ctgaccaagg ccacctgtgc cccccagcat ggggccccgg gtcctgggcc tgctgacgcc
     8401 agcaaggtgg tggccaaggg cctggggctg agcaaggcct acgtaggcca gaagagcagc
     8461 ttcacagtag actgcagcaa agcaggcaac aacatgctgc tggtgggggt tcatggccca
     8521 aggaccccct gcgaggagat cctggtgaag cacgtgggca gccggctcta cagcgtgtcc
     8581 tacctgctca aggacaaggg ggagtacaca ctggtggtca aatgggggca cgagcacatc
     8641 ccaggcagcc cctaccgcgt tgtggtgccc taaaagcttc gaattctgca gtcgacggta
     8701 ccgcgggccc gggatccacc ggatctagat aactgatcat aatcagccat accacagt
//
