LOCUS       sequence-88513-e        8688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8688)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 8688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: mEos2-FilaminA-N-9
FEATURES             Location/Qualifiers
     source          1..8688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7969..8649
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gacgtcagat ccgctagcgc caccatgagt agctcccact ctcgggcggg ccagagcgca
       61 gcaggcgcgg ctccgggcgg cggcgtcgac acgcgggacg ccgagatgcc ggccaccgag
      121 aaggacctgg cggaggacgc gccatggaag aagatccagc agaacacttt cacgcgctgg
      181 tgcaacgagc acctgaagtg cgtgagcaag cgcatcgcca acctgcagac ggacctgagc
      241 gacgggctgc ggcttatcgc gctgttggag gtgctcagcc agaagaagat gcaccgcaag
      301 cacaaccagc ggcccacttt ccgccaaatg cagcttgaga acgtgtcggt ggcactcgag
      361 ttcctggacc gcgagagcat caaactggtg tccatcgaca gcaaggccat cgtggacggg
      421 aacctgaagc tgatcctggg cctcatctgg accctgatcc tgcactactc catctccatg
      481 cccatgtggg acgaggagga ggatgaggag gccaagaagc agacccccaa gcagaggctc
      541 ctgggctgga tccagaacaa gctgccgcag ctgcccatca ccaacttcag ccgggactgg
      601 cagagcggcc gggccctggg cgccctggtg gacagctgtg ccccgggcct gtgtcctgac
      661 tgggactctt gggacgccag caagcccgtt accaatgcgc gagaggccat gcagcaggcg
      721 gatgactggc tgggcatccc ccaggtgatc acccccgagg agattgtgga ccccaacgtg
      781 gacgagcact ctgtcatgac ctacctgtcc cagttcccca aggccaagct gaagccaggg
      841 gctcccttgc ggcccaaact gaacccgaag aaagcccgtg cctacgggcc aggcatcgag
      901 cccacaggca acatggtgaa gaagcgggca gagttcactg tggagaccag aagtgctggc
      961 cagggagagg tgctggtgta cgtggaggac ccggccggac accaggagga ggcaaaagtg
     1021 accgccaata acgacaagaa ccgcaccttc tccgtctggt acgtccccga ggtgacgggg
     1081 actcataagg ttactgtgct ctttgctggc cagcacatcg ccaagagccc cttcgaggtg
     1141 tacgtggata agtcacaggg tgacgccagc aaagtgacag cccaaggtcc cggcctggag
     1201 cccagtggca acatcgccaa caagaccacc tactttgaga tctttacggc aggagctggc
     1261 acgggcgagg tcgaggttgt gatccaggac cccatgggac agaagggcac ggtagagcct
     1321 cagctggagg cccggggcga cagcacatac cgctgcagct accagcccac catggagggc
     1381 gtccacaccg tgcacgtcac gtttgccggc gtgcccatcc ctcgcagccc ctacactgtc
     1441 actgttggcc aagcctgtaa cccgagtgcc tgccgggcgg ttggccgggg cctccagccc
     1501 aagggtgtgc gggtgaagga gacagctgac ttcaaggtgt acacaaaggg cgctggcagt
     1561 ggggagctga aggtcaccgt gaagggcccc aagggagagg agcgcgtgaa gcagaaggac
     1621 ctgggggatg gcgtgtatgg cttcgagtat taccccatgg tccctggaac ctatatcgtc
     1681 accatcacgt ggggtggtca gaacatcggg cgcagtccct tcgaagtgaa ggtgggcacc
     1741 gagtgtggca atcagaaggt acgggcctgg ggccctgggc tggagggcgg cgtcgttggc
     1801 aagtcagcag actttgtggt ggaggctatc ggggacgacg tgggcacgct gggcttctcg
     1861 gtggaagggc catcgcaggc taagatcgaa tgtgacgaca agggcgacgg ctcctgtgat
     1921 gtgcgctact ggccgcagga ggctggcgag tatgccgttc acgtgctgtg caacagcgaa
     1981 gacatccgcc tcagcccctt catggctgac atccgtgacg cgccccagga cttccaccca
     2041 gacagggtga aggcacgtgg gcctggattg gagaagacag gtgtggccgt caacaagcca
     2101 gcagagttca cagtggatgc caagcacggt ggcaaggccc cacttcgggt ccaagtccag
     2161 gacaatgaag gctgccctgt ggaggcgttg gtcaaggaca acggcaatgg cacttacagc
     2221 tgctcctacg tgcccaggaa gccggtgaag cacacagcca tggtgtcctg gggaggcgtc
     2281 agcatcccca acagcccctt cagggtgaat gtgggagctg gcagccaccc caacaaggtc
     2341 aaagtatacg gccccggagt agccaagaca gggctcaagg cccacgagcc cacctacttc
     2401 actgtggact gcgccgaggc tggccagggg gacgtcagca tcggcatcaa gtgtgcccct
     2461 ggagtggtag gccccgccga agctgacatc gacttcgaca tcatccgcaa tgacaatgac
     2521 accttcacgg tcaagtacac gccccggggg gctggcagct acaccattat ggtcctcttt
     2581 gctgaccagg ccacgcccac cagccccatc cgagtcaagg tggagccctc tcatgacgcc
     2641 agtaaggtga aggccgaggg ccctggcctc agtcgcactg gtgtcgagct tggcaagccc
     2701 acccacttca cagtaaatgc caaagctgct ggcaaaggca agctggacgt ccagttctca
     2761 ggactcacca agggggatgc agtgcgagat gtggacatca tcgaccacca tgacaacacc
     2821 tacacagtca agtacacgcc tgtccagcag ggtccagtag gcgtcaatgt cacttatgga
     2881 ggggatccca tccctaagag ccctttctca gtggcagtat ctccaagcct ggacctcagc
     2941 aagatcaagg tgtctggcct gggagagaag gtggacgttg gcaaagacca ggagttcaca
     3001 gtcaaatcaa agggtgctgg tggtcaaggc aaagtggcat ccaagattgt gggcccctcg
     3061 ggtgcagcgg tgccctgcaa ggtggagcca ggcctggggg ctgacaacag tgtggtgcgc
     3121 ttcctgcccc gtgaggaagg gccctatgag gtggaggtga cctatgacgg cgtgcccgtg
     3181 cctggcagcc cctttcctct ggaagctgtg gcccccacca agcctagcaa ggtgaaggcg
     3241 tttgggccgg ggctgcaggg aggcagtgcg ggctcccccg cccgcttcac catcgacacc
     3301 aagggcgccg gcacaggtgg cctgggcctg acggtggagg gcccctgtga ggcgcagctc
     3361 gagtgcttgg acaatgggga tggcacatgt tccgtgtcct acgtgcccac cgagcccggg
     3421 gactacaaca tcaacatcct cttcgctgac acccacatcc ctggctcccc attcaaggcc
     3481 cacgtggttc cctgctttga cgcatccaaa gtcaagtgct caggccccgg gctggagcgg
     3541 gccaccgctg gggaggtggg ccaattccaa gtggactgct cgagcgcggg cagcgcggag
     3601 ctgaccattg agatctgctc ggaggcgggg cttccggccg aggtgtacat ccaggaccac
     3661 ggtgatggca cgcacaccat tacctacatt cccctctgcc ccggggccta caccgtcacc
     3721 atcaagtacg gcggccagcc cgtgcccaac ttccccagca agctgcaggt ggaacctgcg
     3781 gtggacactt ccggtgtcca gtgctatggg cctggtattg agggccaggg tgtcttccgt
     3841 gaggccacca ctgagttcag tgtggacgcc cgggctctga cacagaccgg agggccgcac
     3901 gtcaaggccc gtgtggccaa cccctcaggc aacctgacgg agacctacgt tcaggaccgt
     3961 ggcgatggca tgtacaaagt ggagtacacg ccttacgagg agggactgca ctccgtggac
     4021 gtgacctatg acggcagtcc cgtgcccagc agccccttcc aggtgcccgt gaccgagggc
     4081 tgcgacccct cccgggtgcg tgtccacggg ccaggcatcc aaagtggcac caccaacaag
     4141 cccaacaagt tcactgtgga gaccagggga gctggcacgg gcggcctggg cctggctgta
     4201 gagggcccct ccgaggccaa gatgtcctgc atggataaca aggacggcag ctgctcggtc
     4261 gagtacatcc cttatgaggc tggcacctac agcctcaacg tcacctatgg tggccatcaa
     4321 gtgccaggca gtcctttcaa ggtccctgtg catgatgtga cagatgcgtc caaggtcaag
     4381 tgctctgggc ccggcctgag cccaggcatg gttcgtgcca acctccctca gtccttccag
     4441 gtggacacaa gcaaggctgg tgtggcccca ttgcaggtca aagtgcaagg gcccaaaggc
     4501 ctggtggagc cagtggacgt ggtagacaac gctgatggca cccagaccgt caattatgtg
     4561 cccagccgag aagggcccta cagcatctca gtactgtatg gagatgaaga ggtaccccgg
     4621 agccccttca aggtcaaggt gctgcctact catgatgcca gcaaggtgaa ggccagtggc
     4681 cccgggctca acaccactgg cgtgcctgcc agcctgcccg tggagttcac catcgatgca
     4741 aaggacgccg gggagggcct gctggctgtc cagatcacgg atcccgaagg caagccgaag
     4801 aagacacaca tccaagacaa ccatgacggc acgtatacag tggcctacgt gccagacgtg
     4861 acaggtcgct acaccatcct catcaagtac ggtggtgacg agatcccctt ctccccgtac
     4921 cgcgtgcgtg ccgtgcccac cggggacgcc agcaagtgca ctgtcacagg tgctggcatc
     4981 ggccccacca ttcagattgg ggaggagacg gtgatcactg tggacactaa ggcggcaggc
     5041 aaaggcaaag tgacgtgcac cgtgtgcacg cctgatggct cagaggtgga tgtggacgtg
     5101 gtggagaatg aggacggcac tttcgacatc ttctacacgg ccccccagcc gggcaaatac
     5161 gtcatctgtg tgcgctttgg tggcgagcac gtgcccaaca gccccttcca agtgacggct
     5221 ctggctgggg accagccctc ggtgcagccc cctctacggt ctcagcagct ggccccacag
     5281 tacacctacg cccagggcgg ccagcagact tgggccccgg agaggcccct ggtgggtgtc
     5341 aatgggctgg atgtgaccag cctgaggccc tttgaccttg tcatcccctt caccatcaag
     5401 aagggcgaga tcacagggga ggttcggatg ccctcaggca aggtggcgca gcccaccatc
     5461 actgacaaca aagacggcac cgtgaccgtg cggtatgcac ccagcgaggc tggcctgcac
     5521 gagatggaca tccgctatga caacatgcac atcccaggaa gccccttgca gttctatgtg
     5581 gattacgtca actgtggcca tgtcactgcc tatgggcctg gcctcaccca tggagtagtg
     5641 aacaagcctg ccaccttcac cgtcaacacc aaggatgcag gagagggggg cctgtctctg
     5701 gccattgagg gcccgtccaa agcagaaatc agctgcactg acaaccagga tgggacatgc
     5761 agcgtgtcct acctgcctgt gctgccgggg gactacagca ttctagtcaa gtacaatgaa
     5821 cagcacgtcc caggcagccc cttcactgcc cgggtcacag gtgacgactc catgcgtatg
     5881 tcccacctaa aggtcggctc tgctgccgac atccccatca acatctcaga gacggatctc
     5941 agcctgctga cggccactgt ggtcccgccc tcgggccggg aggagccctg tttgctgaag
     6001 cggctgcgta atggccacgt ggggatttca ttcgtgccca aggagacggg ggagcacctg
     6061 gtgcatgtga agaaaaatgg ccagcacgtg gccagcagcc ccatcccggt ggtgatcagc
     6121 cagtcggaaa ttggggatgc cagtcgtgtt cgggtctctg gtcagggcct tcacgaaggc
     6181 cacacctttg agcctgcaga gtttatcatt gatacccgcg atgcaggcta tggtgggctc
     6241 agcctgtcca ttgagggccc cagcaaggtg gacatcaaca cagaggacct ggaggacggg
     6301 acgtgcaggg tcacctactg ccccacagag ccaggcaact acatcatcaa catcaagttt
     6361 gccgaccagc acgtgcctgg cagccccttc tctgtgaagg tgacaggcga gggccgggtg
     6421 aaagagagca tcacccgcag gcgtcgggct ccttcagtgg ccaacgttgg tagtcattgt
     6481 gacctcagcc tgaaaatccc tgaaattagc atccaggata tgacagccca ggtgaccagc
     6541 ccatcgggca agacccatga ggccgagatc gtggaagggg agaaccacac ctactgcatc
     6601 cgctttgttc ccgctgagat gggcacacac acagtcagcg tcaagtacaa gggccagcac
     6661 gtgcctggga gccccttcca gttcaccgtg gggcccctag gggaaggggg agcccacaag
     6721 gtccgagctg ggggccctgg cctggagaga gctgaagctg gagtgccagc cgaattcagt
     6781 atctggaccc gggaagctgg tgctggaggc ctggccattg ctgtcgaggg ccccagcaag
     6841 gctgagatct cttttgagga ccgcaaggac ggctcctgtg gtgtggctta tgtggtccag
     6901 gagccaggtg actacgaagt ctcagtcaag ttcaacgagg aacacattcc cgacagcccc
     6961 ttcgtggtgc ctgtggcttc tccgtctggc gacgcccgcc gcctcactgt ttctagcctt
     7021 caggagtcag ggctaaaggt caaccagcca gcctcttttg cagtcagcct gaacggggcc
     7081 aagggggcga tcgatgccaa ggtgcacagc ccctcaggag ccctggagga gtgctatgtc
     7141 acagaaattg accaagataa gtatgctgtg cgcttcatcc ctcgggagaa tggcgtttac
     7201 ctgattgacg tcaagttcaa cggtacccac atccctggaa gccccttcaa gatccgagtt
     7261 ggggagcctg ggcatggagg ggacccaggc ttggtgtctg cttacggagc aggtctggaa
     7321 ggcggtgtca cagggaaccc agctgagttc gtcgtgaaca cgagcaatgc gggagctggt
     7381 gccctgtcgg tgaccattga cggcccctcc aaggtgaaga tggattgcca ggagtgccct
     7441 gagggctacc gcgtcaccta tacccccatg gcacctggca gctacctcat ctccatcaag
     7501 tacggcggcc cctaccacat tgggggcagc cccttcaagg ccaaagtcac aggcccccgt
     7561 ctcgtcagca accacagcct ccacgagaca tcatcagtgt ttgtagactc tctgaccaag
     7621 gccacctgtg ccccccagca tggggccccg ggtcctgggc ctgctgacgc cagcaaggtg
     7681 gtggccaagg gcctggggct gagcaaggcc tacgtaggcc agaagagcag cttcacagta
     7741 gactgcagca aagcaggcaa caacatgctg ctggtggggg ttcatggccc aaggaccccc
     7801 tgcgaggaga tcctggtgaa gcacgtgggc agccggctct acagcgtgtc ctacctgctc
     7861 aaggacaagg gggagtacac actggtggtc aaatgggggc acgagcacat cccaggcagc
     7921 ccctaccgcg ttgtggtgcc cagcggcagc gcctcaccgg tcgccaccat gagtgcgatt
     7981 aagccagaca tgaagatcaa actccgtatg gaaggcaacg taaacgggca ccactttgtg
     8041 atcgacggag atggtacagg caagcctttt gagggaaaac agagtatgga tcttgaagtc
     8101 aaagagggcg gacctctgcc ttttgccttt gatatcctga ccactgcatt ccattacggc
     8161 aacagggtat tcgccaaata tccagacaac atacaagact attttaagca gtcgtttcct
     8221 aaggggtatt cgtgggaacg aagcttgact ttcgaagacg ggggcatttg cattgccaga
     8281 aacgacataa caatggaagg ggacactttc tataataaag ttcgatttta tggtaccaac
     8341 tttcccgcca atggtccagt tatgcagaag aagacgctga aatgggagcc ctccactgag
     8401 aaaatgtatg tgcgtgatgg agtgctgacg ggtgatattc atatggcttt gttgcttgaa
     8461 ggaaatgccc attaccgatg tgacttcaga actacttaca aagctaagga gaagggtgtc
     8521 aagttaccag gctaccactt tgtggaccac tgcattgaga ttttaagcca tgacaaagat
     8581 tacaacaagg ttaagctgta tgagcatgct gttgctcatt ctggattgcc tgacaatgcc
     8641 agacgataag cggccgcgac tctagatcat aatcagccat accacatt
//
