LOCUS       sequence-88518-e        1195 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: mEos2-H2B-6
FEATURES             Location/Qualifiers
     source          1..1195
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..456
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             475..1155
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 accgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgccaccat gccagagcca gcgaagtctg ctcccgcccc gaaaaagggc
      121 tccaagaagg cggtgactaa ggcgcagaag aaaggcggca agaagcgcaa gcgcagccgc
      181 aaggagagct attccatcta tgtgtacaag gttctgaagc aggtccaccc tgacaccggc
      241 atttcgtcca aggccatggg catcatgaat tcgtttgtga acgacatttt cgagcgcatc
      301 gcaggtgagg cttcccgcct ggcgcattac aacaagcgct cgaccatcac ctccagggag
      361 atccagacgg ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc cgtgtccgag
      421 ggtactaagg ccatcaccaa gtacaccagc gctaaggatc caccggtcgc caccatgagt
      481 gcgattaagc cagacatgaa gatcaaactc cgtatggaag gcaacgtaaa cgggcaccac
      541 tttgtgatcg acggagatgg tacaggcaag ccttttgagg gaaaacagag tatggatctt
      601 gaagtcaaag agggcggacc tctgcctttt gcctttgata tcctgaccac tgcattccat
      661 tacggcaaca gggtattcgc caaatatcca gacaacatac aagactattt taagcagtcg
      721 tttcctaagg ggtattcgtg ggaacgaagc ttgactttcg aagacggggg catttgcatt
      781 gccagaaacg acataacaat ggaaggggac actttctata ataaagttcg attttatggt
      841 accaactttc ccgccaatgg tccagttatg cagaagaaga cgctgaaatg ggagccctcc
      901 actgagaaaa tgtatgtgcg tgatggagtg ctgacgggtg atattcatat ggctttgttg
      961 cttgaaggaa atgcccatta ccgatgtgac ttcagaacta cttacaaagc taaggagaag
     1021 ggtgtcaagt taccaggcta ccactttgtg gaccactgca ttgagatttt aagccatgac
     1081 aaagattaca acaaggttaa gctgtatgag catgctgttg ctcattctgg attgcctgac
     1141 aatgccagac gataagcggc cgcgactcta gatcataatc agccatacca cattg
//
