LOCUS       sequence-88530-e        2045 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosome Membrane, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: mEos2-Lysosomes-20
FEATURES             Location/Qualifiers
     source          1..2045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      43..52
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(45..107,108..1265)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     CDS             1326..2006
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gtcagatccg ctagcgctac cggactcaga tctcgagcgc caccatggcg gccccgggcg
       61 cccggcgtcc gctgctcctg ttgctgctgg caggccttgc acacagcgcc ccagcactgt
      121 tcgaggtgaa agacaacaac ggcacagcgt gtataatggc cagcttctct gcctcctttc
      181 tgaccaccta tgaggctgga catgtttcta aggtctcgaa tatgaccctg ccagcctctg
      241 cagaagtcct gaagaatagc agctcttgtg gtgaaaagaa tgcttctgag cccaccctcg
      301 caatcacctt tggagaagga tatttactga aactcacctt cacaaaaaac acaacacgtt
      361 acagtgtcca gcacatgtat ttcacatata acctgtcaga cacacaattc tttcccaatg
      421 ccagctccaa agggcccgac actgtggatt ccacaactga catcaaggca gacatcaaca
      481 aaacataccg atgtgtcagc gacatcaggg tctacatgaa gaatgtgacc attgtgctct
      541 gggacgctac tatccaggcc tacctgccga gtagcaactt cagcaaggaa gagacacgct
      601 gcccacagga tcaaccttcc ccaactactg ggccacccag cccctcacca ccacttgtgc
      661 ccacaaaccc cagtgtgtcc aagtacaatg tgactggtga caatggaacc tgcctgctgg
      721 cctctatggc actgcaactc aacatcacct acatgaagaa ggacaacacg actgtgacca
      781 gagcattcaa catcaaccca agtgacaaat atagtgggac ttgcggtgcc cagttggtga
      841 ccctgaaggt ggggaacaag agcagagtcc tggagctgca gtttgggatg aatgccactt
      901 ctagcctgtt tttcctgcaa ggagttcagt tgaacatgac tcttcctgat gccatagagc
      961 ccacgttcag cacctccaac tattccctga aagctcttca ggccagtgtc ggcaactcat
     1021 acaagtgcaa cagtgaggag cacatctttg tcagcaaggc gctcgccctc aatgtcttca
     1081 gcgtgcaagt ccaggctttc agggtagaaa gtgacaggtt tgggtctgtg gaagagtgtg
     1141 tacaggacgg taacaacatg ctgatcccca ttgctgtggg cggggccctg gcagggctgg
     1201 tcctcatcgt cctcatcgcc tacctcatcg gcaggaagag gagtcacgcg ggctatcaga
     1261 ccatctcgga attcggctcc accggctcca ccggctccac cggcgcggat ccaccggtcg
     1321 ccaccatgag tgcgattaag ccagacatga agatcaaact ccgtatggaa ggcaacgtaa
     1381 acgggcacca ctttgtgatc gacggagatg gtacaggcaa gccttttgag ggaaaacaga
     1441 gtatggatct tgaagtcaaa gagggcggac ctctgccttt tgcctttgat atcctgacca
     1501 ctgcattcca ttacggcaac agggtattcg ccaaatatcc agacaacata caagactatt
     1561 ttaagcagtc gtttcctaag gggtattcgt gggaacgaag cttgactttc gaagacgggg
     1621 gcatttgcat tgccagaaac gacataacaa tggaagggga cactttctat aataaagttc
     1681 gattttatgg taccaacttt cccgccaatg gtccagttat gcagaagaag acgctgaaat
     1741 gggagccctc cactgagaaa atgtatgtgc gtgatggagt gctgacgggt gatattcata
     1801 tggctttgtt gcttgaagga aatgcccatt accgatgtga cttcagaact acttacaaag
     1861 ctaaggagaa gggtgtcaag ttaccaggct accactttgt ggaccactgc attgagattt
     1921 taagccatga caaagattac aacaaggtta agctgtatga gcatgctgtt gctcattctg
     1981 gattgcctga caatgccaga cgataagcgg ccgcgactct agatcataat cagccatacc
     2041 acatt
//
