LOCUS       sequence-88533-e        1834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1834)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos2-MCHR1-N-10
FEATURES             Location/Qualifiers
     source          1..1834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1115..1795
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gacggtcaga tccgctagcg ccaccatgga tctgcaagcc tcgttgctgt ccactggccc
       61 caatgccagc aacatctccg atggccagga taatttcaca ttggcggggc cacctcctcg
      121 cacaaggagt gtctcctaca tcaacatcat catgccttca gtgtttggta ccatctgtct
      181 cctgggcatt gtgggaaact ccacagtcat ttttgccgtg gtgaagaaat ccaagctgca
      241 ctggtgcagc aacgtccctg acatcttcat catcaacctc tctgtggtgg atctgctttt
      301 cctgctgggc atgcctttca tgatccacca gctcatgggt aatggtgtct ggcactttgg
      361 ggaaaccatg tgcaccctca tcacagccat ggacgccaac agtcagttca ccagcaccta
      421 catcctgact gctatggcca ttgaccgcta cttggccacc gtccatccca tctcctccac
      481 caagttccgg aagccctcca tggccaccct ggtgatctgc ctcctgtggg ctctctcgtt
      541 cattagcatc actcctgtgt ggctctatgc caggcttatc cccttcccag ggggtgctgt
      601 gggctgtggc atccgcctac caaacccaga tactgatctt tactggttca ctctgtatca
      661 gtttttcctg gccttcgccc ttccgtttgt ggtcatcact gctgcgtacg tgaaaatact
      721 acagcgcatg acgtcttcgg tggccccagc ctctcaacgc agcatccggc ttcggacaaa
      781 gagggtgacc cgcacagcca ttgccatctg tctggtcttc tttgtgtgct gggcgcccta
      841 ctacgtgctg cagctgaccc agttgtccat cagccgcccg accctcacat tcgtctacct
      901 gtacaatgcg gctatcagct tgggctatgc caacagctgc ctcaatccct ttgtgtacat
      961 agtactctgt gagacctttc gaaaacgctt ggtgctgtcg gtgaagcccg cggcccaggg
     1021 gcagcttcgc acggtcagca atgctcagac agctgacgag gagaggacag aaagcaaagg
     1081 caccggaggt agcggggatc caccggtcgc caccatgagt gcgattaagc cagacatgaa
     1141 gatcaaactc cgtatggaag gcaacgtaaa cgggcaccac tttgtgatcg acggagatgg
     1201 tacaggcaag ccttttgagg gaaaacagag tatggatctt gaagtcaaag agggcggacc
     1261 tctgcctttt gcctttgata tcctgaccac tgcattccat tacggcaaca gggtattcgc
     1321 caaatatcca gacaacatac aagactattt taagcagtcg tttcctaagg ggtattcgtg
     1381 ggaacgaagc ttgactttcg aagacggggg catttgcatt gccagaaacg acataacaat
     1441 ggaaggggac actttctata ataaagttcg attttatggt accaactttc ccgccaatgg
     1501 tccagttatg cagaagaaga cgctgaaatg ggagccctcc actgagaaaa tgtatgtgcg
     1561 tgatggagtg ctgacgggtg atattcatat ggctttgttg cttgaaggaa atgcccatta
     1621 ccgatgtgac ttcagaacta cttacaaagc taaggagaag ggtgtcaagt taccaggcta
     1681 ccactttgtg gaccactgca ttgagatttt aagccatgac aaagattaca acaaggttaa
     1741 gctgtatgag catgctgttg ctcattctgg attgcctgac aatgccagac gataagcggc
     1801 cgcgactcta gatcataatc agccatacca catt
//
