LOCUS       sequence-88534-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: mEos2-Mito-7
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        4..55
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     6..26
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             84..158
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             192..872
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 catgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt
       61 agtgaaccgt cagatccgct agcatgtccg tcctgacgcc gctgctgctg cggggcttga
      121 caggctcggc ccggcggctc ccagtgccgc gcgccaagat ccattcgttg ggggatccac
      181 cggtcgccac catgagtgcg attaagccag acatgaagat caaactccgt atggaaggca
      241 acgtaaacgg gcaccacttt gtgatcgacg gagatggtac aggcaagcct tttgagggaa
      301 aacagagtat ggatcttgaa gtcaaagagg gcggacctct gccttttgcc tttgatatcc
      361 tgaccactgc attccattac ggcaacaggg tattcgccaa atatccagac aacatacaag
      421 actattttaa gcagtcgttt cctaaggggt attcgtggga acgaagcttg actttcgaag
      481 acgggggcat ttgcattgcc agaaacgaca taacaatgga aggggacact ttctataata
      541 aagttcgatt ttatggtacc aactttcccg ccaatggtcc agttatgcag aagaagacgc
      601 tgaaatggga gccctccact gagaaaatgt atgtgcgtga tggagtgctg acgggtgata
      661 ttcatatggc tttgttgctt gaaggaaatg cccattaccg atgtgacttc agaactactt
      721 acaaagctaa ggagaagggt gtcaagttac caggctacca ctttgtggac cactgcattg
      781 agattttaag ccatgacaaa gattacaaca aggttaagct gtatgagcat gctgttgctc
      841 attctggatt gcctgacaat gccagacgat aagcggccgc gactctagat cataatcagc
      901 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
      961 ctgaaacata aaatgaatgc aattgttgtt gtta
//
