LOCUS       sequence-88537-e        6763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6763)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos2-MyosinIIB-N-18
FEATURES             Location/Qualifiers
     source          1..6763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      63..72
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6051..6731
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca gtcgacggta
       61 ccgccaccat ggcgcagaga actggactcg aggatccaga gaggtatctc tttgtggaca
      121 gggctgtcat ctacaaccct gccactcaag ctgattggac agctaaaaag ctagtgtgga
      181 ttccatcaga acgccatggt tttgaggcag ctagtatcaa agaagaacgg ggagatgaag
      241 ttatggtgga gttggcagag aatggaaaga aagcaatggt caacaaagat gatattcaga
      301 agatgaaccc acctaagttt tccaaggtgg aggatatggc agaattgaca tgcttgaatg
      361 aagcttccgt tttacataat ctgaaggatc gctactattc aggactaatc tatacttatt
      421 ctggactctt ctgtgtagtt ataaaccctt acaagaatct tccaatttac tctgagaata
      481 ttattgaaat gtacagaggg aagaagcgtc atgagatgcc tccacacatc tatgctatat
      541 ctgaatctgc ttacagatgc atgcttcaag atcgtgagga ccagtcaatt ctttgcacgg
      601 gtgagtcagg tgctgggaag acagaaaata caaagaaagt tattcagtac cttgcccatg
      661 ttgcttcttc acataaagga agaaaggacc ataatattcc tggggaactt gaacggcagc
      721 ttttgcaagc aaatccaatt ctggaatcat ttggaaatgc gaagactgtg aaaaatgata
      781 actcatctcg ttttggcaaa tttattcgga tcaactttga tgtaactggc tatatcgttg
      841 gggccaacat tgaaacatac cttctggaaa agtctcgtgc tgttcgtcaa gcaaaagatg
      901 aacgtacttt tcatatcttt taccagttgt tatctggagc aggagaacac ctaaagtctg
      961 atttgcttct tgaaggattt aataactaca ggtttctctc caatggctat attcctattc
     1021 cgggacagca agacaaagat aatttccagg agaccatgga agcaatgcac ataatgggct
     1081 tctcccatga agagattctg tcaatgctta aagtagtatc ttcagtgcta cagtttggaa
     1141 atatttcttt caaaaaggag agaaatactg atcaagcttc catgccagaa aatacagttg
     1201 cgcagaagct ctgccatctt cttgggatga atgtgatgga gtttactcgg gccatcctga
     1261 ctccccggat caaggtcggc cgagactatg tgcaaaaagc ccagaccaaa gaacaggcag
     1321 attttgcagt agaagcattg gcaaaagcta cctatgagcg gctctttcgc tggctcgttc
     1381 atcgcatcaa taaagctctg gataggacca aacgtcaggg agcatctttc attggaatcc
     1441 tggatattgc tggatttgaa atttttgagc tgaactcctt tgaacaactt tgcatcaact
     1501 acaccaatga gaagctgcag cagctgttca accacaccat gtttatccta gaacaagagg
     1561 aataccagcg cgaaggcatc gagtggaact tcatcgattt cgggctggat ctgcagccat
     1621 gcatcgacct aatagagaga cctgcgaacc ctcctggtgt actggccctt ttggatgaag
     1681 aatgctggtt ccctaaagcc acagataaaa cctttgttga aaaactggtt caagagcaag
     1741 gttcccactc caagtttcag aaacctcgac aattaaaaga caaagctgat ttttgcatta
     1801 tacattatgc agggaaggtg gactataagg cagatgagtg gctgatgaag aatatggacc
     1861 ccctgaatga caacgtggcc acccttttgc accagtcatc agacagattt gtggcagagc
     1921 tttggaaaga tgtggaccgt atcgtgggtc tggatcaagt cactggtatg actgagacag
     1981 cttttggctc cgcatataaa accaagaagg gcatgtttcg taccgttggg caactctaca
     2041 aagaatctct caccaagctg atggcaactc tccgaaacac caaccctaac tttgttcgtt
     2101 gtatcattcc aaatcacgag aagagggctg gaaaattgga tccacaccta gtcctagatc
     2161 agcttcgctg taatggtgtc ctggaaggga tccgaatctg tcgccagggc ttccctaacc
     2221 gaatagtttt ccaggaattc agacagagat atgagatcct aactccaaat gctattccta
     2281 aaggttttat ggatggtaaa caggcctgtg aacgaatgat ccgggcttta gaattggacc
     2341 caaacttgta cagaattgga cagagcaaga tatttttcag agctggagtt ctggcacact
     2401 tagaggaaga aagagattta aaaatcaccg atatcattat cttcttccag gccgtttgca
     2461 gaggttacct ggccagaaag gcctttgcca agaagcagca gcaactaagt gccttaaagg
     2521 tcttgcagcg gaactgtgcc gcgtacctga aattacggca ctggcagtgg tggcgagtct
     2581 tcacaaaggt gaagccgctt ctacaagtga ctcgccagga ggaagaactt caggccaaag
     2641 atgaagagct gttgaaggtg aaggagaagc agacaaaggt ggaaggagag ctggaggaga
     2701 tggagcggaa gcaccagcag cttttagaag agaagaatat ccttgcagaa caactacaag
     2761 cagagactga gctctttgct gaagcagaag agatgagggc aagacttgct gctaaaaagc
     2821 aggaattaga agagattcta catgacttgg agtctagggt tgaagaagaa gaagaaagaa
     2881 accaaatcct ccaaaatgaa aagaaaaaaa tgcaagcaca tattcaggac ctggaagaac
     2941 agctagacga ggaggaaggg gctcggcaaa agctgcagct ggaaaaggtg acagcagagg
     3001 ccaagatcaa gaagatggaa gaggagattc tgcttctcga ggaccaaaat tccaagttca
     3061 tcaaagaaaa gaaactcatg gaagatcgca ttgctgagtg ttcctctcag ctggctgaag
     3121 aggaagaaaa ggcgaaaaac ttggccaaaa tcaggaataa gcaagaagtg atgatctcag
     3181 atctagaaga acgcttaaag aaggaagaaa agactcgtca ggaactggaa aaggccaaaa
     3241 gaaaactcga cggggagacg accgacctgc aggaccagat cgcagagctg caggcgcaga
     3301 ttgatgagct caagctgcag ctggccaaga aggaggagga gctgcagggc gcactggcca
     3361 gaggtgatga tgaaacactc cataagaaca atgcccttaa agttgtgcga gagctacaag
     3421 cccaaattgc tgaacttcag gaagactttg aatccgagaa ggcttcacgg aacaaggccg
     3481 aaaagcagaa aagggacttg agtgaggaac tggaagctct gaaaacagag ctggaggaca
     3541 cgctggacac cacggcagcc cagcaggaac tacgtacaaa acgtgaacaa gaagtggcag
     3601 agctgaagaa agctcttgag gaggaaacta agaaccatga agctcaaatc caggacatga
     3661 gacaaagaca cgcaacagcc ctggaggagc tctcagagca gctggaacag gccaagcggt
     3721 tcaaagcaaa tctagagaag aacaagcagg gcctggagac agataacaag gagctggcgt
     3781 gtgaggtgaa ggtcctgcag caggtcaagg ctgagtctga gcacaagagg aagaagctcg
     3841 acgcgcaggt ccaggagctc catgccaagg tctctgaagg cgacaggctc agggtggagc
     3901 tggcggagaa agcaagtaag ctgcagaatg agctagataa tgtctccacc cttctggaag
     3961 aagcagagaa gaagggtatt aaatttgcta aggatgcagc tagtcttgag tctcaactac
     4021 aggatacaca ggagcttctt caggaggaga cacgccagaa actaaacctg agcagtcgga
     4081 tccggcagct ggaagaggag aagaacagtc ttcaggagca gcaggaggag gaggaggagg
     4141 ccaggaagaa cctggagaag caagtgctgg ccctgcagtc ccagttggct gataccaaga
     4201 agaaagtaga tgacgacctg ggaacaattg aaagtctgga agaagccaag aagaagcttc
     4261 tgaaggacgc ggaggccctg agccagcgcc tggaggagaa ggcactggcg tatgacaaac
     4321 tggagaagac caagaaccgc ctgcagcagg agctggacga cctcacggtg gacctggacc
     4381 accagcgcca ggtcgcctcc aacttggaga agaagcagaa gaagtttgac cagctgttag
     4441 cagaagagaa gagcatctct gctcgctatg ccgaagagcg ggaccgggcc gaagccgagg
     4501 ccagagagaa agaaaccaaa gccctgtcac tggcccgggc cctcgaggaa gccctggagg
     4561 ccaaggagga gtttgagagg cagaacaagc agctccgagc agacatggaa gacctcatga
     4621 gctccaaaga tgatgtggga aaaaacgttc acgaacttga aaaatccaaa cgggccctag
     4681 agcagcaggt ggaggaaatg aggacccagc tggaggagct ggaagacgaa ctccaggcca
     4741 cggaagatgc caagcttcgt ctggaggtca acatgcaggc catgaaggcg cagttcgaga
     4801 gagacctgca aaccagggat gagcagaatg aagagaagaa gcggctgctg atcaaacagg
     4861 tgcgggagct cgaggcggag ctggaggatg agaggaaaca gcgggcgctt gctgtagctt
     4921 caaagaaaaa gatggagata gacctgaagg acctcgaagc ccaaatcgag gctgcgaaca
     4981 aagctcggga tgaggtgatt aagcagctcc gcaagctcca ggctcagatg aaggattacc
     5041 aacgtgaatt agaagaagct cgtgcatcca gagatgagat ttttgctcaa tccaaagaga
     5101 gtgaaaagaa attgaagagt ctggaagcag aaatccttca attgcaggag gaacttgcct
     5161 catctgagcg agcccgccga cacgccgagc aggagagaga tgagctggcg gacgagatca
     5221 ccaacagcgc ctctggcaag tccgcgctgc tggatgagaa gcggcgtctg gaagctcgga
     5281 tcgcacagct ggaggaggag ctggaagagg agcagagcaa catggagctg ctcaacgacc
     5341 gcttccgcaa gaccactcta caggtggaca cactgaacgc cgagctagca gccgagcgca
     5401 gcgccgccca gaagagtgac aatgcacgcc agcaactgga gcggcagaac aaggagctga
     5461 aggccaagct gcaggaactc gagggtgctg tcaagtctaa gttcaaggcc accatctcag
     5521 ccctggaggc caagattggg cagctggagg agcagcttga gcaggaagcc aaggaacgag
     5581 cagccgccaa caaattagtc cgtcgcactg agaagaagct gaaagaaatc ttcatgcagg
     5641 ttgaggatga gcgtcgacac gcggaccagt ataaagagca gatggagaag gccaacgctc
     5701 ggatgaagca gcttaaacgc cagctggagg aagcagaaga agaagcgacg cgtgccaacg
     5761 catctcggcg taaactccag cgggaactgg atgatgccac cgaggccaac gagggcctga
     5821 gccgcgaggt cagcaccctg aagaaccggc tgaggcgggg tggccccatc agcttctctt
     5881 ccagccgatc tggccggcgc cagctgcacc ttgaaggagc ttccctggag ctctccgacg
     5941 atgacacaga aagtaagacc agtgatgtca acgagacgca gccaccccag tcagagggca
     6001 gcggtggagg cagcgcatcc ggcggaagcg gaagcggacc ggtcgccacc atgagtgcga
     6061 ttaagccaga catgaagatc aaactccgta tggaaggcaa cgtaaacggg caccactttg
     6121 tgatcgacgg agatggtaca ggcaagcctt ttgagggaaa acagagtatg gatcttgaag
     6181 tcaaagaggg cggacctctg ccttttgcct ttgatatcct gaccactgca ttccattacg
     6241 gcaacagggt attcgccaaa tatccagaca acatacaaga ctattttaag cagtcgtttc
     6301 ctaaggggta ttcgtgggaa cgaagcttga ctttcgaaga cgggggcatt tgcattgcca
     6361 gaaacgacat aacaatggaa ggggacactt tctataataa agttcgattt tatggtacca
     6421 actttcccgc caatggtcca gttatgcaga agaagacgct gaaatgggag ccctccactg
     6481 agaaaatgta tgtgcgtgat ggagtgctga cgggtgatat tcatatggct ttgttgcttg
     6541 aaggaaatgc ccattaccga tgtgacttca gaactactta caaagctaag gagaagggtg
     6601 tcaagttacc aggctaccac tttgtggacc actgcattga gattttaagc catgacaaag
     6661 attacaacaa ggttaagctg tatgagcatg ctgttgctca ttctggattg cctgacaatg
     6721 ccagacgata agcggccgcg actctagatc ataatcagcc ata
//
