LOCUS       sequence-88538-e        6786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6786)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos2-MyosinIIC-N-18
FEATURES             Location/Qualifiers
     source          1..6786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6070..6750
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gctagcaccg ccaccatggc tgcagtgacc atgtccgtgt ctgggaggaa ggtagcctcc
       61 aggccaggcc cggtgcctga ggcagcccaa tcgttcctct acgcgccccg gacgccaaat
      121 gtaggtggcc ctggagggcc acaggtggag tggacagccc ggcgcatggt gtgggtgccc
      181 tcggaactgc atgggttcga ggcagcagcc ctgcgggatg aaggggagga ggaggcagaa
      241 gtggagctgg cggagagtgg gcgccgcctg cggctgccca gggaccagat ccagcgcatg
      301 aacccaccca agttcagcaa ggcagaagat atggctgagc tcacctgcct caacgaggcc
      361 tcggtcctgc acaacctgcg agaacgctac tactccgggc tcatttatac ctactctggc
      421 ctcttctgtg tggtcattaa cccatacaag cagctgccca tctacacgga ggccattgtt
      481 gaaatgtacc ggggcaagaa gcgccatgag gtgccacctc acgtgtatgc tgtgacggag
      541 ggcgcgtacc gcagcatgct tcaggatcgt gaggatcaat ccattctctg cacgggagag
      601 tctggcgctg ggaagacgga gaacaccaag aaggtcatcc agtacctggc ccatgtggca
      661 tcatctccaa agggcaggaa ggagcctggt gtccctgcct ccgtcagcac catgtcttat
      721 ggggagctag agcgtcagct tcttcaagcc aaccccatcc tagaggcctt tggcaatgcc
      781 aagacagtga agaacgacaa ctcttcccga tttggcaaat tcatccgcat caactttgat
      841 attgctggct acatcgtggg agcaaacatc gagacctatc tgttggagaa gtcccgggcc
      901 atcagacagg ccaaggatga atgcagcttc catatcttct accagctgct agggggcgct
      961 ggggagcagc taaaagctga cctccttctg gagccctgtt cccattatcg cttcctgacc
     1021 aatgggccct catcgtcccc gggccaggag cgtgagttat tccaggagac cctggagtcc
     1081 ctgcgtgtgc tgggcctcct cccagaagag atcactgcca tgctgcgcac tgtctctgct
     1141 gtcctccagt ttggcaacat tgtcctgaag aaagagcgca atacggacca agccaccatg
     1201 cctgacaaca cagctgccca gaagctttgc cgcctcttgg gactcggagt gaccgacttc
     1261 tccagagccc ttctcacacc ccgcatcaaa gtgggccgag attatgttca gaaagcacaa
     1321 accaaggagc aggctgactt tgcgctggag gctctggcca aagctaccta tgagcgcctg
     1381 ttccgctggc tggttctgcg gctcaaccgt gccctggaca gaagcccgcg gcagggtgcc
     1441 tccttcctgg gcatcctgga catcgcgggc tttgagatct tccagctgaa ctccttcgag
     1501 cagctgtgca tcaactacac caacgagaag ctacagcagc tattcaacca caccatgttc
     1561 gtgctggagc aggaggagta ccagcgagag ggcatcccct ggaccttcct agacttcggg
     1621 ttggacctgc aaccttgcat cgacctcatt gagcgtccgg ccaaccctcc aggtctcctg
     1681 gccctgctgg acgaggagtg ctggttcccc aaggccacgg acaagtcttt tgtggagaag
     1741 gtcgcccagg agcagggcag ccaccccaaa ttccagcgcc ccaggaacct gcgagatcag
     1801 gccgacttca gcgtcctgca ctacgccggc aaggttgact acaaagccag tgagtggctg
     1861 atgaagaaca tggacccact gaatgacaat gtggccgcct tgcttcacca gagcacggat
     1921 cgtctcacag ctgagatctg gaaggatgtg gagggcatcg tggggctgga gcaagtaagc
     1981 agccttggag atggcccacc gggaggccgc ccccgccgtg gaatgtttcg gactgtgggg
     2041 cagctctaca aagaatccct gagccgcctc atggccacgc tcagcaacac caaccctagt
     2101 tttgtccgct gcatcgttcc caatcatgag aagagggctg gaaagctgga gccgcgcctg
     2161 gtgctggacc aactccgttg taacggggtc ctcgagggta tacgcatctg tcgccaaggc
     2221 ttccccaacc gcatcctctt ccaggagttc cgacagcgct atgaaatcct caccccgaac
     2281 gctattccca agggcttcat ggacggcaaa caggcctgtg agaagatgat ccaggccctg
     2341 gagctagacc ccaacctgta ccgtgttggc caaagcaaga tcttcttccg ggcaggggtc
     2401 ctggcccagc tggaggagga gcgggacctg aaagtcaccg acatcatagt gtctttccag
     2461 gcagcggcac ggggctacct ggcccgtagg gctttccaga gacggcagca gcagcagagt
     2521 gctctgaggg tgatgcagag aaactgtgct gcctacctca agctcaggaa ctggcagtgg
     2581 tggaggctgt tcatcaaggt gaagcccctg ctgcaggtga cacggcagga tgaggtgctg
     2641 caggcgcgcg cccaggagct gcagaaagtt caggagctgc agcagcagag cgctcgtgaa
     2701 gtgggggaac tgcagggtcg agtggcacag ctagaggagg agcgcacgcg cctggctgag
     2761 cagcttcgag cagaagccga gctctgctct gaggccgagg agacgcgggc gcgactggct
     2821 gcccggaagc aggagctgga gctggtggtg acagagctgg aggcacgagt gggcgaggaa
     2881 gaagagtgca gccggcagct gcagagtgag aagaagaggc tgcagcagca tatccaggag
     2941 ctagagagcc acctggaagc tgaggagggt gcccggcaga agctacagct ggagaaggtg
     3001 accacagagg ccaagatgaa gaaatttgag gaggacctgc tgctcctgga ggaccagaat
     3061 tccaagctga gcaaggagcg gaggctgctg gaggagcggc tggctgagtt ctcctcacag
     3121 gcagcagaag aggaagagaa agtcaaaagt ctcaacaagc tgaggctcaa atatgaagcc
     3181 acaatctcag acatggaaga ccggctgaag aaggaggaga agggacgcca ggaactagag
     3241 aagctgaagc gacggctgga cggggagagc tcagagcttc aggagcagat ggtggagcag
     3301 aagcagaggg cagaggaact gctcgcacag ctgggccgca aggaggatga gctgcaggcc
     3361 gccctgctca gggcagagga agagggtggt gcccgtgccc agttgctcaa gtccctgcga
     3421 gaggcacagg ctggccttgc tgaggctcag gaggacctgg aagctgagcg ggtagccagg
     3481 gccaaggcgg agaagcagcg ccgggacctg ggcgaggagt tggaggccct acgtggggag
     3541 ctcgaggaca ctctggattc caccaacgcc cagcaggagc tgcggtccaa gagggagcag
     3601 gaggtgacag agctgaagaa agcattggaa gaggagtccc gtgcccatga ggtgtccatg
     3661 caggagctga gacagaggca tagccaggca ctggtggaga tggccgagca gttggagcaa
     3721 gcccggaggg gcaaaggtgt gtgggagaag actcggctat ccctggaggc tgaggtgtcc
     3781 gagctgaagg ccgagctgag cagcctgcag acctcgagac aggagggtga gcagaagagg
     3841 cgccgcctgg agtcccagct acaggaggtc cagggccgat ccagtgattc ggagcgggct
     3901 cggtctgagg ctgctgagaa gctgcagaga gcccaggcgg aacttgagag cgtgtccaca
     3961 gccctgagtg aggcggagtc caaagccatc aggctgggca aggagctgag cagtgcagag
     4021 tcccagctgc atgacaccca ggaactgctt caggaggaga ccagggcaaa gctggccttg
     4081 gggtcccgtg tgcgtgccct agaggccgag gcggcggggc ttcgggagca gatggaagag
     4141 gaggtggttg ccagggaacg ggctggccgg gagctgcaga gcacgcaggc ccagctctct
     4201 gaatggcggc gccgccagga agaagaggct gcggtgctgg aggctgggga ggaggctcgg
     4261 cgccgtgcag cccgggaggc agagaccctg acccagcgcc tggcagaaaa gactgaggct
     4321 gtagaacgac tggagcgagc ccggcgccga ctgcagcagg agttggacga tgccactgtg
     4381 gatctggggc agcagaagca gctcctgagc acactggaga agaagcagcg gaaatttgac
     4441 cagctcctgg cagaggagaa ggctgcagtt ctacgggctg tggaagaccg tgaacggata
     4501 gaggccgaag gccgggagcg agaggcccgg gccctgtcgc tgacccgggc cctggaagag
     4561 gagcaggagg cccgggagga gctggagagg cagaaccgtg ctctgagggc tgagctggaa
     4621 gcactgctga gcagcaagga tgacgtgggc aagaacgtgc acgagctgga gcgagcccgt
     4681 aaggcggctg aacaggcagc cagtgacctg cggacacagg tgacagaatt ggaggatgag
     4741 ctgacagccg cagaggatgc caagctgcgc ctggaggtga ctgtgcaggc tctgaaggct
     4801 caacatgaac gcgacctgca gggccgcgat gatgccggtg aggagaggcg gaggcagctg
     4861 gccaagcagc taagagacgc agaggtagag cgcgatgagg aacggaagca gagggcactg
     4921 gctatggctg cccgcaagaa gctggagctg gaactggagg agttgaaggc gcagacatct
     4981 gctgctgggc agggcaagga agaggcagtg aagcagctga agaagatgca ggtccagatg
     5041 aaggagctgt ggcgggaggt agaggagacg cgtagctccc gcgacgagat gtttaccctg
     5101 agcagggaaa atgagaagaa gctcaagggg ctggaagctg aggtgctgcg tctgcaagag
     5161 gaacttgctg cctcagaccg agcccggagg caggcccagc aagacagaga cgagatggca
     5221 gaggaggtgg ccagtggcaa tcttagcaag gcagccaccc tggaggaaaa acggcagctg
     5281 gaggggcgac tgagccagtt ggaagaggag ctggaggaag aacagaacaa ctcggagctg
     5341 ctcaaggacc attaccgaaa gctagtgcta caggtcgagt ccctcaccac agaactgtct
     5401 gccgaacgaa gtttctcagc caaggccgag agtggacggc agcagctgga gcggcagatc
     5461 caggaactgc gggcccgctt gggtgaagag gatgctggag cccgagccag gcagaaaatg
     5521 ctgatcgctg ctctggagtc taaactggcc caggcagagg agcagctgga gcaggagagc
     5581 agggagcgca tcctctctgg caagctggta cgcagagctg agaagcggct gaaggaggta
     5641 gttcttcagg tggatgaaga gcgcagggtg gctgaccagg tccgggacca gctggagaaa
     5701 agcaacctcc ggctgaagca gctcaagagg cagctggagg aggcagagga ggaggcatct
     5761 cgggcacagg ctggtcggag gcggctgcag cgggagctgg aggacgtcac tgagtctgca
     5821 gaatccatga accgggaggt gaccacgctg aggaacaggc tccggcgtgg cccacttaca
     5881 ttcaccacac ggactgtgcg ccaggtgttc cggctggaag agggcgtggc ttctgacgag
     5941 gaagaggctg aaggagctga acctggctct gcaccaggcc aggagccgga ggctccgccc
     6001 cctgccacac cccaaagcag cggtggagca agcgcagcca gtggtagcgc ggatccaccg
     6061 gtcgccacca tgagtgcgat taagccagac atgaagatca aactccgtat ggaaggcaac
     6121 gtaaacgggc accactttgt gatcgacgga gatggtacag gcaagccttt tgagggaaaa
     6181 cagagtatgg atcttgaagt caaagagggc ggacctctgc cttttgcctt tgatatcctg
     6241 accactgcat tccattacgg caacagggta ttcgccaaat atccagacaa catacaagac
     6301 tattttaagc agtcgtttcc taaggggtat tcgtgggaac gaagcttgac tttcgaagac
     6361 gggggcattt gcattgccag aaacgacata acaatggaag gggacacttt ctataataaa
     6421 gttcgatttt atggtaccaa ctttcccgcc aatggtccag ttatgcagaa gaagacgctg
     6481 aaatgggagc cctccactga gaaaatgtat gtgcgtgatg gagtgctgac gggtgatatt
     6541 catatggctt tgttgcttga aggaaatgcc cattaccgat gtgacttcag aactacttac
     6601 aaagctaagg agaagggtgt caagttacca ggctaccact ttgtggacca ctgcattgag
     6661 attttaagcc atgacaaaga ttacaacaag gttaagctgt atgagcatgc tgttgctcat
     6721 tctggattgc ctgacaatgc cagacgataa gcggccgcga ctctagatca taatcagcca
     6781 taccac
//
