LOCUS       sequence-88544-e        2960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Scaffold, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2960)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos2-PSD95-N-14
FEATURES             Location/Qualifiers
     source          1..2960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2247..2927
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 tagcgctacc ggactcagat ctcgaggcca ccatggactg tctctgtata gtgacaacca
       61 agaaataccg ctaccaagat gaagacacgc cccctctgga acacagcccg gcccacctcc
      121 ccaaccaggc caattctccc cctgtgattg tcaacacgga caccctagaa gccccaggat
      181 atgagttgca ggtgaatgga acagaggggg agatggagta tgaggagatc acattggaaa
      241 ggggtaactc aggtctgggc ttcagcatcg caggtggcac tgacaacccg cacatcggtg
      301 acgacccgtc catttttatc accaagatca ttcctggtgg ggctgcagcc caggatggcc
      361 gcctcagggt caatgacagc atcctgtttg taaatgaagt ggatgttcgg gaggtgaccc
      421 attcagctgc ggtggaggcc ctcaaagagg caggttccat cgttcgcctc tatgtcatgc
      481 gccggaaacc cccagccgaa aaggtcatgg agatcaaact catcaaaggg cctaaaggac
      541 ttggcttcag cattgcgggg ggcgttggga accagcacat ccctggagat aacagcatct
      601 atgtaacgaa gatcatcgaa ggaggtgctg cccacaagga tggcaggttg cagattggag
      661 acaagatcct ggcggtcaac agtgtggggc tggaggacgt catgcacgag gatgccgtgg
      721 cagccctgaa gaacacatat gacgttgtgt acctaaaggt ggccaagccc agcaatgcct
      781 acctgagtga cagctatgct cccccagaca tcacaacctc gtattctcag cacctggaca
      841 atgagatcag tcatagcagc tacttgggca ctgactaccc cacagccatg acccccactt
      901 cccctcggcg ctactcccct gtggccaagg acctgctggg ggaggaagac attccccggg
      961 aaccaaggcg gatcgtgatc catcggggct ccaccggcct gggcttcaac atcgtgggcg
     1021 gcgaggatgg tgaaggcatc ttcatctcct tcatccttgc tgggggtcca gccgacctca
     1081 gtggggagct acggaagggg gaccagatcc tgtcggtcaa tggtgttgac ctccgcaatg
     1141 ccagtcacga acaggctgcc attgccctga agaatgcggg tcagacggtc acgatcatcg
     1201 ctcagtataa accagaagag tatagtcgat tcgaggccaa gatccatgat cttcgggaac
     1261 agctcatgaa tagtagccta ggctcaggga ctgcatcctt gcgaagcaac cccaagaggg
     1321 gcttctacat tagggccctg tttgattacg acaagaccaa ggactgcggt ttcttgagcc
     1381 aggccctgag cttccgcttc ggggatgtgc ttcatgtcat tgacgctggt gacgaagagt
     1441 ggtggcaagc acggcgggtc cactccgaca gtgagaccga cgacattggc ttcattccca
     1501 gcaaacggcg ggtcgagcga cgagagtggt caaggttaaa ggccaaggac tggggctcca
     1561 gctctggatc acagggtcga gaagactcgg ttctgagcta tgagacggtg acccagatgg
     1621 aagtgcacta tgctcgtccc atcatcatcc ttggacccac caaagaccgt gccaacgatg
     1681 atcttctctc cgagttcccc gacaagtttg gatcctgtgt ccctcatacg acacgtccta
     1741 agcgggaata tgagatagac ggccgggatt accactttgt ctcctcccgg gagaaaatgg
     1801 agaaggacat ccaggcacac aagttcattg aggctggcca gtacaacagc cacctctatg
     1861 ggaccagcgt ccagtctgtg cgagaggtag cagagcaggg gaagcactgc atcctcgatg
     1921 tctcggccaa tgccgtgcgg cggctgcagg cggcccacct gcaccccatc gccatcttca
     1981 tccgtccccg ctccctggag aatgtgctag agatcaataa gcggatcaca gaggagcaag
     2041 cccggaaagc cttcgacaga gccacgaagc tggagcagga gttcacagag tgcttctcag
     2101 ccatcgtaga gggcgacagc tttgaagaga tctatcacaa agtgaaacgt gtcattgaag
     2161 acctctcagg cccctacatc tgggtcccag cccgagagag actcggcagc gcatccggcg
     2221 gaagcggaag cggaccggtc gccaccatga gtgcgattaa gccagacatg aagatcaaac
     2281 tccgtatgga aggcaacgta aacgggcacc actttgtgat cgacggagat ggtacaggca
     2341 agccttttga gggaaaacag agtatggatc ttgaagtcaa agagggcgga cctctgcctt
     2401 ttgcctttga tatcctgacc actgcattcc attacggcaa cagggtattc gccaaatatc
     2461 cagacaacat acaagactat tttaagcagt cgtttcctaa ggggtattcg tgggaacgaa
     2521 gcttgacttt cgaagacggg ggcatttgca ttgccagaaa cgacataaca atggaagggg
     2581 acactttcta taataaagtt cgattttatg gtaccaactt tcccgccaat ggtccagtta
     2641 tgcagaagaa gacgctgaaa tgggagccct ccactgagaa aatgtatgtg cgtgatggag
     2701 tgctgacggg tgatattcat atggctttgt tgcttgaagg aaatgcccat taccgatgtg
     2761 acttcagaac tacttacaaa gctaaggaga agggtgtcaa gttaccaggc taccactttg
     2821 tggaccactg cattgagatt ttaagccatg acaaagatta caacaaggtt aagctgtatg
     2881 agcatgctgt tgctcattct ggattgcctg acaatgccag acgataagcg gccgcgactc
     2941 tagatcataa tcagccatac
//
