LOCUS       sequence-88553-e        6342 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane/Actin, Excitation: 505 / 569, Emission: 516 /
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6342)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos2-Supervillin-45
FEATURES             Location/Qualifiers
     source          1..6342
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..707
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 cagatccgct agcgctaccg gtcgccacca tgagtgcgat taagccagac atgaagatca
       61 aactccgtat ggaaggcaac gtaaacgggc accactttgt gatcgacgga gatggtacag
      121 gcaagccttt tgagggaaaa cagagtatgg atcttgaagt caaagagggc ggacctctgc
      181 cttttgcctt tgatatcctg accactgcat tccattacgg caacagggta ttcgccaaat
      241 atccagacaa catacaagac tattttaagc agtcgtttcc taaggggtat tcgtgggaac
      301 gaagcttgac tttcgaagac gggggcattt gcattgccag aaacgacata acaatggaag
      361 gggacacttt ctataataaa gttcgatttt atggtaccaa ctttcccgcc aatggtccag
      421 ttatgcagaa gaagacgctg aaatgggagc cctccactga gaaaatgtat gtgcgtgatg
      481 gagtgctgac gggtgatatt catatggctt tgttgcttga aggaaatgcc cattaccgat
      541 gtgacttcag aactacttac aaagctaagg agaagggtgt caagttacca ggctaccact
      601 ttgtggacca ctgcattgag attttaagcc atgacaaaga ttacaacaag gttaagctgt
      661 atgagcatgc tgttgctcat tctggattgc ctgacaatgc cagacgatcc ggactcagat
      721 ctcgagctca agcttcgaat tctgcagtcg acggtacctt cgatgctcag cttttagctg
      781 cccttcctaa agttgcagaa ttaagaaaaa tctttgaacc aaagaggaaa gaatttttag
      841 aaatgaaaag aaaagaaaga attgcccggc gcttagaagg aattgaaacc gacacgcagc
      901 ccatcctctt gcagagctgc acgggcttgg tgactcatcg cctgctggag gaggacacac
      961 ccagatacat gcgtgccaca gacccggcca gcccgcacat tggtcgatca aatgaagaag
     1021 aagaaacttc agattcttca ctagaaaagc agactcgatc caaacagtgc acagaaacct
     1081 caggcatcca tgctgactcg ccctacagtt caggcatcat ggacacccag agcctcgagt
     1141 ccaaagccga aagaatcgcc aggtacaaag cagagagaag acggcagctg gcagaaaagt
     1201 atgggctcac cctggatccg gaagcagact ctgaaactcc atctcgatac agcaggtcca
     1261 gaaaggaccc cgaggctgcg gagaaaaggg gagtaagaag cgagcgatcg gccgagtcca
     1321 gcagagacgc cggctcatcc tactccagga ctgagctctc ggggctcagg acctgcgtgg
     1381 ctgagtccaa ggactanggc ctgcaccgga gtgacggtgt ttccgacaca gaggtgctgc
     1441 tcaatgcaga aaaccaaaga cgcggtcaag agcccagtgc caccgggctg gcccgagacc
     1501 tgccccttgc aggggaggtc tcctcttctt tctcattctc cgggcgagac tctgccctcg
     1561 gtgaagtgcc aaggtcccca aaggcagtgc acagcctgcc cagtccatcg cctgggcagc
     1621 cggcctcccc aagccactcc accagtgacc tgccactccc tgcggaggct cgagccaggt
     1681 caacttcaaa ttcagaaatg ccagctgccg aggatgaaga aaaggtcgat gagcgagcga
     1741 ggctgagcgt cgctgctaag aggctgctct tcagggagat ggagaagtcg tttgacgaga
     1801 agagcgtccc aaagcgccgc tcccgcaatg cggccgtgga gcagaggctg cggcgcctcc
     1861 aggaccggtc ccacacgcag cccgtcacca ccgaggaggt ggtcattgca gccacattgc
     1921 aggcgtctgc tcaccaaaag gcactagcca gagaccagac aaacgagagc aaagattctg
     1981 ctgagcaggg agaacctgac tcctccactc tgagcttagc ggagaagctg gccttgttta
     2041 acaaactatc tcagccggtc tcaaaagcca tttcaacacg gaacagatta gacatgagac
     2101 agaggaggat gaatgctcgt tatcaaaccc agccggtcac gcttggagaa gtggaacagg
     2161 tgcagagtgg caagctcatg gctttctctc ccaccattaa cacttccgtg tccaccgtgg
     2221 catctaccgt cccccccatg tatgcaggaa atcttcggac aaagccactt ccagatgaca
     2281 gctttggtgc cactgagcag aagtttgctt cttcgctaga aaactccgac tccccagtta
     2341 gaagcatcct gaaatcccaa ggctggcagc cctcggtcga gggtgctggg agcaaagcaa
     2401 tgttgagaga atttgaagag acagaacgca agggaggctt gacaggtgga gatggcgggg
     2461 ttacaaagta tgggtccttc gaggaagcag agctgtccta ccccgtcctc agcagagtcc
     2521 gggagggaga caaccataaa gaggccatct atgcccttcc gaggaaagga agcctggagc
     2581 tcgcccatcc tcccatcgcc cagcttggtg atgacctgaa ggaattttcc accccaaaga
     2641 gcaccatgca ggcaagccca gactggaagg agaggcagct ctttgaagag aaggtggact
     2701 tggagaatgt cacaaagagg aagttttcgc tgaaagccgc ggagttcggg gaacccactt
     2761 ctgagcagac cggcgcggct gctgggaaac cggctgctcc gactgcaacc ccggtgtcct
     2821 ggaagccgca ggatccctcc gaacagcctc aggagaagcg gtatcagagc ccgtgtgcga
     2881 tgtttgctgc tggagagatc aaagccccgg cggtggaggg cagcctcgac tcccccagca
     2941 aaaccatgtc cattaaagaa agattagcac tgttgaagaa gagcggtgag gaagactgga
     3001 gaaacagact caacagaaag caggaatacg gcaaagcctc catcaccagc agcctgcaca
     3061 tccaagagac ggagcagtca ctcaagaaga agcgggtcac agaaagtcga gagagccaga
     3121 tgactattga agagagaaag catctcatca ccgtgagaga ggacgcctgg aagaccaggg
     3181 gcaaaggagc agccaacgac tctacccagt ttactgtggc cggcaggatg gtgaagcgag
     3241 gtctggcgtc ccccactgcc atcaccccgg tagcatcccc tgtttccagc aaagcaaggg
     3301 gcactacacc agtttccaga cccctggaag atatcgaagc cagaccagac atgcagttag
     3361 agtcggacct caagctggac aggctggaaa ccttcctaag aaggctgaat aacaaagttg
     3421 gtgggatgca agaaacagtc ctcactgtca ctgggaaatc ggtgaaagag gtgatgaagc
     3481 cggacgatga tgaaaccttt gccaagtttt accgcagcgt ggactcctct ctaccgagaa
     3541 gccccgtaga gctggacgag gatttcgatg tcatttttga tccttatgcc cccaggttga
     3601 catcctcggt ggctgagcac aagcgtgccg tgcggcccaa gcgccgggtc caggcttcca
     3661 aaaaccccct gaagatgctg gccgcgaggg aagatctcct gcaggagtac actgagcaga
     3721 gactgaacgt ggccttcgtg gagtcaaagc ggatgaaagt cgaaaagttg tccgccaact
     3781 ccagcttctc agaagtcacc ctggcggggt tagccagcaa agaaaacttc agcaacgtca
     3841 gcctgcggag cgtcaacctg acggaacaga attccaacaa cagtgcagtg ccctacaaga
     3901 agctgatgct gctgcaggtt aaaggaagaa gacacgtgca gacgcggctg gtggagcctc
     3961 gcgccccctc cctcaacagc ggggactgct tcctcctgct ctcgccccat cactgcttcc
     4021 tgtgggtcgg agagttcgcc aacgtgatcg agaaggcgaa ggcctcagaa cttgcgagtt
     4081 taattcagac caagagggaa cttggttgta gagcaactta catccagact gttgaagaag
     4141 gaattaatac acacacacat gcagccaaag acttctggaa gcttctgggc ggccaagcca
     4201 gttaccaatc cgccggagac ccaaaggagg atgagctcta tgaaacggcc ataatagaga
     4261 ccaactgcat ttaccgtctg atggatgaca aactcgttcc tgatgatgac tactggggga
     4321 agatcccaaa gtgctccctc ctgcaatcaa aagaggtact ggtgtttgat tttgggagtg
     4381 aagtttacgt gtggcatgga aaagaagtca cattagcaca acggaaaatc gcctttcagc
     4441 tggcaaagca cttgtggaat ggaacctttg actacgagaa ttgtgacatt aacccgctgg
     4501 atcctgggga atgcaatccc ctcattccca ggaaaggaca ggggcggccc gactgggcaa
     4561 tatttgggag acttacagaa cacaatgaga cgatcttgtt caaagagaaa ttccttgatt
     4621 ggacggaact gaagagacct aacgagaaga acgccagcga actcgcgcag cacaaggacg
     4681 atgcccgggc agaggtcaag ccttacgacg tgacgcggat ggtgcccgtg ccccagacaa
     4741 cagccggcac cgtgctggat ggggtcaacg tgggccgagg ctacgggctg gtggaagggg
     4801 acgaccggag gcagttcgag atcgccagca tctcagtgga cgtctggcac atcctggagt
     4861 tcgactacag caggctcccc aagcagagca tcgggcagtt ccacgaaggc gatgcctacg
     4921 tggtgaagtg gaagttcatc gtgagcactg cagtgggaag ccggcagaag ggggagcact
     4981 cggtcagggt ggctggcaaa gagaaatgtg tctacttctt ctggcaaggc cgccagtcga
     5041 ccgtgagtga gaagggcaca tcagctctga tgacagtgga gctggacgag gaaagggggg
     5101 cccaggtcca ggtcctgcag ggcaaggagc ccccctgttt cctgcagtgc ttccaggggg
     5161 ggatggtggt ccactccggg agacgggagg aggaggaaga gaacacacag agcgagtggc
     5221 ggctgtactg tgtgcgcgga gaggtgcctg tggaggggaa cttgctggag gtggcctgtc
     5281 actgcagcag cctcaggtct aggacgtcca tggttgtcct caacgtccac aaagccctca
     5341 tctacctgtg gcatggatgc aaagcccagg cccacacgaa ggaggtcgga cggactgcag
     5401 ccaataaaat caaggaccaa tgtcccctgg aggccgggct gcacagtagc agcaaagtga
     5461 caattcatga gtgtgatgaa ggctcggagc ccctggggtt ctgggacgct ctggggagga
     5521 gagaccggaa agcctacgac tgcatgcttc aagatcctgg aaattttaac ttcacacccc
     5581 gcctgttcat cctcagcagc tcctctggtg acttctcagc cacagagttc atgtatcccg
     5641 cccgagaccc ctctgtggtc aactctatgc ccttcctgca ggaagaccta tacagtgccc
     5701 cacagccagc actcttcctt gttgacaatc accacgaggt gtacctctgg caaggctggt
     5761 ggcccatcga gaacaagatc acgggctccg cgcgcatccg ctgggcctcg gaccggaaga
     5821 gcgccatgga gacagtgctg cagtactgcc gagggaaaaa cctgaagaag ccgcccccca
     5881 agtcttacct catccacgct ggcctggaac ccctgacctt caccaacatg ttccccagct
     5941 gggagcacag agaagacatt gcagagatca cggagatgga cacggaagtt tcaaatcaga
     6001 tcaccctagt ggaggatgtc ttagccaaac tgtgtaagac catttaccca ctggccgacc
     6061 tcctagccag gccgctccct gagggagttg accctctgaa gctggagatc tatctcaccg
     6121 acgaggactt cgagtttgca ctagacatga cccgagacga gtacaatgca ctgcccgctt
     6181 ggaagcaggt gaacctgaag aaagcgaaag gtctgttctg agtcaggaga tgctggagga
     6241 acctgggtgg tcactttcaa tactgatgga ccaggaaaat ggatgtttaa tcgaattccc
     6301 gcggccttct agataactga tcataatcag ccataccaca tt
//
