LOCUS       sequence-88554-e        8459 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8459)
  AUTHORS   Kanchanawong P, Shtengel G, Pasapera AM, Ramko EB, Davidson MW, Hess
            HF, Waterman CM
  TITLE     Nanoscale architecture of integrin-based cell adhesions.
  JOURNAL   Nature. 2010 Nov 25;468(7323):580-4. doi: 10.1038/nature09621.
  PUBMED    21107430
REFERENCE   2  (bases 1 to 8459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-11-25- 25"; volume: "468"; issue: "7323"; 
            pages: "580-4"
COMMENT     Sequence Label: mEos2-Talin-N-22
FEATURES             Location/Qualifiers
     source          1..8459
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      53..62
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7748..8428
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc tagccaccat
       61 ggttgcgctt tcgctgaaga ttagcattgg gaatgtggtg aagacgatgc aatttgagcc
      121 atctaccatg gtgtacgacg cctgccgcat gattcgtgag cggatcccag aggccctggc
      181 tggccctccc aacgactttg ggctctttct gtcagatgat gaccccaaaa aaggcatctg
      241 gctagaggct gggaaagctt tggactacta catgctccga aatggggaca ccatggagta
      301 cagaaaaaag cagagacccc tgaagatacg gatgttagac ggaacggtga agactatcat
      361 ggtggatgac tccaagaccg tcacagacat gctcatgacc atttgtgccc gaattggtat
      421 caccaaccat gatgagtatt cactggttcg agagctgatg gaagaaaaga aagatgaggg
      481 gacagggacc ctgagaaaag acaagaccct actgcgagat gaaaagaaga tggagaagct
      541 aaagcagaag ttgcacacag acgatgagtt gaactggctg gaccatgggc ggacactgag
      601 ggaacaggga gtggaagagc atgagacgtt gctgctgcgg agaaagttct tctactcaga
      661 ccagaatgtg gattcccgag accctgtaca gctgaacctt ctctatgtgc aggcacgaga
      721 tgacatcctg aatggctccc atcctgtctc ctttgacaag gcctgtgaat ttgcaggctt
      781 ccagtgccag atccagtttg gacctcacaa tgaacagaag cacaaggctg gcttccttga
      841 cctgaaggac ttcctgccca aggagtatgt gaagcagaag ggagagcgta agatctttca
      901 ggcacacaag aattgtgggc agatgagtga aattgaggcc aaggtacgct acgtgaagct
      961 ggcccgttcc ctcaagactt acggtgtctc cttcttccta gtcaaggaaa agatgaaggg
     1021 gaagaataaa ctggtgccca ggctgttggg catcactaag gagtgtgtga tgcgtgtgga
     1081 tgagaagacc aaggaggtga ttcaggaatg gagcctcacc aacatcaaac gctgggctgc
     1141 ctctcccaag agctttactc tggactttgg agactatcag gatggctact actcagtaca
     1201 gacgacagaa ggcgagcaga tcgcacagct cattgctggc tacatagata tcatccttaa
     1261 gaagaaaaaa agcaaggacc attttgggct ggagggagat gaagagtcta ctatgttgga
     1321 ggactcagtg tctcccaaaa agtcaacagt ccttcagcag cagtacaacc gagtggggaa
     1381 agtggagcac ggctctgtgg ctctgccagc catcatgcgc tctggagcct ctggtcctga
     1441 gaatttccag gtggggagca tgccacctgc ccagcagcag atcaccagtg gccagatgca
     1501 ccgaggacac atgccacctc tgacttcagc ccagcaggcg ctcactggaa ccattaactc
     1561 cagcatgcag gctgtgcagg ctgcccaggc cactctggat gactttgaga ctctaccccc
     1621 tcttggccag gatgctgcct ccaaagcttg gcgtaagaac aagatggatg aatcaaagca
     1681 tgagatccac tcccaggtag atgccatcac agctggcact gcctctgtgg taaacctgac
     1741 agcaggagat cctgcagaga cagactatac agcagtgggc tgtgcagtca ccaccatctc
     1801 ttccaacctg acggagatgt cccgtggggt gaagctattg gctgccctgc tggaggatga
     1861 aggcggcaat ggccggcccc ttctgcaagc agcaaagggc cttgcagggg ctgtgtcgga
     1921 actgcttcgc agtgctcagc ccgccagcgc tgagcctcgt cagaacctgc tgcaggcagc
     1981 cgggaacgtg ggccaggcca gtggggagct gttgcagcaa attggggaaa gtgacactga
     2041 cccccacttc caggatgttc taatgcagct agcaaacgca gtggcaagtg ctgcagccgc
     2101 cctggtcctc aaggccaaga gtgtggccca gcgcacagag gattccgggc ttcagaccca
     2161 agtgattgct gcagctacgc aatgtgctct gtccacttcc cagctggtgg cctgtaccaa
     2221 ggtggtagca cctacaatca gctcacctgt gtgccaagaa cagctagtcg aggctgggcg
     2281 actggtggcc aaagctgtgg agggctgtgt gtctgcctcg caagcagcta cagaagatgg
     2341 acagctgctt cgaggggtag gagcagcagc cacggctgtc acccaggccc tcaatgagct
     2401 gctgcagcac gtgaaggccc acgccaccgg agctgggcct gccggtcggt atgaccaagc
     2461 cactgatacc atcctcaccg tcactgaaaa catcttcagc tccatgggtg atgctgggga
     2521 gatggtgcgc caggcccgca tcctggccca agccacctca gacctggtca atgctatcaa
     2581 ggccgatgct gagggggaga gtgatctgga gaactctaga aagctcctga gtgctgccaa
     2641 gatcctcgct gatgccaccg ccaagatggt ggaggcggcc aagggagcag ccgcccaccc
     2701 tgacagtgag gaacagcagc agcgactgcg tgaagcagct gaggggcttc gcatggccac
     2761 caatgcagct gcgcagaacg ccatcaagaa gaagttggtg cagcgcctgg agcatgcagc
     2821 caagcaagct gcagcctctg caacacagac cattgctgca gcccaacatg cagcctctgc
     2881 ccccaaggcc tctgcgggcc cccagcccct actggtgcag agctgtaagg ccgtggcaga
     2941 gcagattccg ttgctggtgc agggtgtccg aggaagtcaa gctcagcctg acagccctag
     3001 tgctcagctc gccctcattg ctgccagcca gagcttcctg cagccaggtg ggaagatggt
     3061 ggcagcggca aaagcctcag tgccaacaat tcaggaccag gcttcagcga tgcagctgag
     3121 tcagtgcgca aagaacctag gcactgccct ggccgagctg cgcactgctg ctcagaaggc
     3181 tcaggaggca tgtggacctt tggagatgga ttctgcactg agtgttgtac aaaatctaga
     3241 gaaagaccta caggaaataa aagcagcagc tcgagatggc aagcttaagc ccttacctgg
     3301 ggaaacaatg gagaagtgta cccaagatct tggcaacagc accaaagcgg tgagctctgc
     3361 catcgccaag ctgctggggg agattgccca gggcaacgag aactatgcag gtattgcagc
     3421 tcgggatgta gctggtggac taaggtcact agcccaggct gcgcggggtg tggctgcgct
     3481 gacatcagat cctgcagtgc aggccattgt gcttgacaca gccagcgacg ttctggacaa
     3541 ggccagcagc ctcattgaag aggcaaaaaa agcatctgga cacccagggg acccagaaag
     3601 ccagcaaagg cttgctcagg tagctaaagc agtgacccaa gccctgaacc gctgtgtcag
     3661 ctgcctgcct ggccaaagag acgtggataa tgccctacga gccgttggag atgccagcaa
     3721 gcgactcctg agtgacttgc ttcctccaag cacggggaca tttcaagaag cacagagccg
     3781 attgaatgaa gctgcagctg ggttaaatca ggccgccaca gaactggtgc aagcctcccg
     3841 aggaactcct caggacctgg ctcgggcctc cggccgattt ggacaggact tcagcacctt
     3901 cctggaagct ggtgtggaga tggccggaca ggccccgagc caggaggacc gagcccaggt
     3961 ggtgtccaac ctgaagggca tatccatgtc ttcaagcaaa cttctccttg ccgctaaggc
     4021 cttgtccaca gaccctgctt ctcccaacct caagagtcag ctggctgcgg ctgcccgggc
     4081 agtgacggac agcatcaacc agctcatcac catgtgcacc cagcaggcac ctggccagaa
     4141 ggagtgtgac aatgcacttc ggcagctaga gacagtccga gaactcctgg agaatccagt
     4201 ccagcccatc aacgacatgt cctacttcgg ttgcttggac agtgtcatgg agaactccaa
     4261 ggtcctaggt gaggccatga ctggcatctc ccaaaatgcc aagaatggaa atctgccgga
     4321 gtttggagat gccattgcca cagcctccaa ggctctctgt ggcttcacgg aggcagccgc
     4381 acaggcagca tatctagttg gtgtctctga ccccaacagc caagctggac agcaaggact
     4441 ggtggaaccc acacagtttg cccgtgcaaa ccaggcaatt cagatggcct gtcagagtct
     4501 gggggagcct ggctgtaccc aggcccaggt gttatctgca gccactattg tagccaaaca
     4561 cacatctgca ttgtgtaaca gctgtcgcct ggcttccgct aggactgcca atcccactgc
     4621 caagcgccag tttgtacagt cagccaagga ggtggccaac agtacagcca atcttgtcaa
     4681 gaccatcaag gcactagatg gggacttcac agaagagaac cgtgcccagt gccgagcagc
     4741 cacagcccct ctgctggaag ctgtggataa cctgagtgcc tttgcctcca accctgagtt
     4801 ctccagcgtc cctgcccaga tcagccctga gggcagagca gccatggagc ccattgtaat
     4861 ctctgctaag acaatgttgg agagtgctgg agggctcatc cagacagctc gggcgttagc
     4921 agtcaatccc cgagaccccc cacgttggtc tgtgctagct ggccactctc ggactgtctc
     4981 agattccatc aagaaactta ttacaagcat gagggacaaa gccccagggc agctggagtg
     5041 tgagacagcc attgcggctc tgaacagctg cttgcgggac ctagatcagg cttcgcttgc
     5101 cgctgtcagc cagcagcttg ctccccgtga aggaatctct caagaggctt tgcacaccca
     5161 gatgctcact gcagtgcaag aaatttctca tctcattgag ccgctggcca gcgctgctcg
     5221 ggctgaagcc tcccagctgg gacacaaggt gtcccaaatg gcccagtact ttgaaccact
     5281 caccctggct gcagtgggtg ctgcgtctaa gaccctgagc cacccgcagc agatggcact
     5341 tctggaccag actaaaacgt tggcagagtc tgccttgcag ttgctatata ctgccaagga
     5401 ggctggtggt aaccccaagc aagcagcaca cacccaggaa gccctggagg aggctgtgca
     5461 gatgatgaca gaggccgtag aagacctgac gacgaccctc aacgaagcag ccagtgctgc
     5521 aggagtcgtt ggcggcatgg tggactctat cactcaggcc atcaaccagc tagatgaagg
     5581 acctatgggt gacccagaag gctcattcgt agattaccag acaaccatgg tgaggacagc
     5641 caaggccatt gctgtcactg ttcaggagat ggtaaccaag tcaaacacca gccctgaaga
     5701 gctaggccct cttgccaacc agctgaccag tgactatggc cgactggcct cacaagccaa
     5761 gcctgcagct gtggctgctg aaaatgaaga gataggcgct cacatcaaac accgagtaca
     5821 ggagctgggc catggctgct ctgctctggt caccaaggca ggtgccttgc agtgtagccc
     5881 cagtgatgtc tacaccaaga aggagctcat agagtgtgcc cgcagagtgt cagagaaggt
     5941 ctcccatgtt ctggctgcac tccaggctgg gaatcgtggt acccaggcct gcattacagc
     6001 agccagtgct gtgtctggta tcattgctga cctcgacacc accatcatgt tcgctactgc
     6061 tggcacactt aaccgtgagg gtgctgaaac ttttgctgac caccgggagg gtatcttaaa
     6121 gacagcaaag gttcttgtgg aggacaccaa ggtcctagtg cagaatgcag ctgggagcca
     6181 ggagaagttg gcacaagccg cccagtcctc cgtggccacc attacccgcc tcgctgatgt
     6241 ggtcaagctc ggtgcagcca gcctaggagc cgaagaccct gaaactcagg tggtgctgat
     6301 caatgcagta aaggacgtag ccaaggccct gggtgacctc atcagcgcta cgaaggctgc
     6361 agcgggcaaa gttggggatg accctgcagt gtggcagctc aagaactctg ccaaggtgat
     6421 ggtgaccaat gtgacatcat tgctcaagac agtgaaggct gtggaagatg aggccaccaa
     6481 aggcacacgg gccctagagg caaccacaga gcacatacgt caggaactgg cggtcttctg
     6541 ttccccagag ccacctgcca agacctctac ccctgaagac ttcatccgaa tgaccaaggg
     6601 tattactatg gcaacagcca aagccgttgc tgctggcaat tcctgtcgac aggaagatgt
     6661 cattgccaca gccaatctga gccgacgggc tattgcggac atgcttcggg cttgcaagga
     6721 agcagctttc cacccagaag tggcgcctga tgtacggctc cgagccctgc actatggccg
     6781 ggagtgtgcc aatggttacc tggaactgct ggaccacgtg ctgctgaccc ttcagaagcc
     6841 aaacccagac ctgaagcagc agctaactgg acactcaaag cgggttgctg gctctgtgac
     6901 tgagctcatc caggctgctg aagccatgaa aggaacagag tgggtggacc cagaggaccc
     6961 tactgtcatt gctgagaatg agctcttggg agccgcagcc gccatcgagg ctgcagccaa
     7021 gaagctggag cagctgaagc ccagggccaa acccaaggag gcggatgagt ccttgaactt
     7081 tgaggaacaa atcctagaag ctgccaagtc catcgctgca gccaccagtg cactggtaaa
     7141 ggctgcgtca gcagcccaga gggagctggt ggctcaagga aaggtgggcg ccattccagc
     7201 caatgcactg gatgatgggc agtggtcgca gggcctcatt tccgctgccc gtatggtggc
     7261 tgcggccacc aacaatctgt gtgaagcagc taacgcagct gtccagggcc atgctagcca
     7321 ggagaaactc atttcctcag ccaagcaagt agctgcctcc acagctcagc tcctggtagc
     7381 ttgcaaggtc aaagcagatc aggactctga ggcaatgaaa cggctccagg ctgctggcaa
     7441 tgcagtgaag agggcctcag ataacctggt aaaggcggcc cagaaagctg cagccttcga
     7501 agaccaggag aatgagacgg tggtggtgaa ggagaaaatg gttgggggca ttgcccagat
     7561 tatcgcagca caggaagaga tgcttcggaa ggaacgagag ctggaagagg ctcggaaaaa
     7621 gctcgcccag atccggcagc agcagtacaa gttcttgcct tcagagcttc gagacgagca
     7681 cggcgctggg tccgggtccg ggcaagggtc cggcgcaggc tccgcgtccc gatcaccggt
     7741 cgccaccatg agtgcgatta agccagacat gaagatcaaa ctccgtatgg aaggcaacgt
     7801 aaacgggcac cactttgtga tcgacggaga tggtacaggc aagccttttg agggaaaaca
     7861 gagtatggat cttgaagtca aagagggcgg acctctgcct tttgcctttg atatcctgac
     7921 cactgcattc cattacggca acagggtatt cgccaaatat ccagacaaca tacaagacta
     7981 ttttaagcag tcgtttccta aggggtattc gtgggaacga agcttgactt tcgaagacgg
     8041 gggcatttgc attgccagaa acgacataac aatggaaggg gacactttct ataataaagt
     8101 tcgattttat ggtaccaact ttcccgccaa tggtccagtt atgcagaaga agacgctgaa
     8161 atgggagccc tccactgaga aaatgtatgt gcgtgatgga gtgctgacgg gtgatattca
     8221 tatggctttg ttgcttgaag gaaatgccca ttaccgatgt gacttcagaa ctacttacaa
     8281 agctaaggag aagggtgtca agttaccagg ctaccacttt gtggaccact gcattgagat
     8341 tttaagccat gacaaagatt acaacaaggt taagctgtat gagcatgctg ttgctcattc
     8401 tggattgcct gacaatgcca gacgataagc ggccgcgact ctagatcata atcagccat
//
