LOCUS       sequence-88558-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Kanchanawong P, Shtengel G, Pasapera AM, Ramko EB, Davidson MW, Hess
            HF, Waterman CM
  TITLE     Nanoscale architecture of integrin-based cell adhesions.
  JOURNAL   Nature. 2010 Nov 25;468(7323):580-4. doi: 10.1038/nature09621.
  PUBMED    21107430
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-11-25- 25"; volume: "468"; issue: "7323"; 
            pages: "580-4"
COMMENT     Sequence Label: mEos2-Talin-IBS2-7
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..52
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     3..23
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      81..90
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             183..863
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt
       61 gaaccgtcag atccgctagc gccaccatgg gccaggtggt gctgatcaac gccgtgaagg
      121 acgtggccaa ggccctgggc gacctgatca gcgccaccaa gggggatcca ccggtcgcca
      181 ccatgagtgc gattaagcca gacatgaaga tcaaactccg tatggaaggc aacgtaaacg
      241 ggcaccactt tgtgatcgac ggagatggta caggcaagcc ttttgaggga aaacagagta
      301 tggatcttga agtcaaagag ggcggacctc tgccttttgc ctttgatatc ctgaccactg
      361 cattccatta cggcaacagg gtattcgcca aatatccaga caacatacaa gactatttta
      421 agcagtcgtt tcctaagggg tattcgtggg aacgaagctt gactttcgaa gacgggggca
      481 tttgcattgc cagaaacgac ataacaatgg aaggggacac tttctataat aaagttcgat
      541 tttatggtac caactttccc gccaatggtc cagttatgca gaagaagacg ctgaaatggg
      601 agccctccac tgagaaaatg tatgtgcgtg atggagtgct gacgggtgat attcatatgg
      661 ctttgttgct tgaaggaaat gcccattacc gatgtgactt cagaactact tacaaagcta
      721 aggagaaggg tgtcaagtta ccaggctacc actttgtgga ccactgcatt gagattttaa
      781 gccatgacaa agattacaac aaggttaagc tgtatgagca tgctgttgct cattctggat
      841 tgcctgacaa tgccagacga taagcggccg cgactctaga tcataatcag ccataccaca
      901 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
      961 aaaatgaatg caattgttgt tgttaacttg ttta
//
