LOCUS       sequence-88559-e        2595 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2595)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos2-TBRII-N-18
FEATURES             Location/Qualifiers
     source          1..2595
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1370..1381
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             1870..2550
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 cgccatggtt aagtgacggt cagatccgct agcgccacca tgggtcgggg gctgctcagg
       61 ggcctgtggc cgctgcacat cgtcctgtgg acgcgtatcg ccagcacgat cccaccgcac
      121 gttcagaagt cggatgtgga aatggaggcc cagaaagatg aaatcatctg ccccagctgt
      181 aataggactg cccatccact gagacatatt aataacgaca tgatagtcac tgacaacaac
      241 ggtgcagtca agtttccaca actgtgtaaa ttttgtgatg tgagattttc cacctgtgac
      301 aaccagaaat cctgcatgag caactgcagc atcacctcca tctgtgagaa gccacaggaa
      361 gtctgtgtgg ctgtatggag aaagaatgac gagaacataa cactagagac agtttgccat
      421 gaccccaagc tcccctacca tgactttatt ctggaagatg ctgcttctcc aaagtgcatt
      481 atgaaggaaa aaaaaaagcc tggtgagact ttcttcatgt gttcctgtag ctctgatgag
      541 tgcaatgaca acatcatctt ctcagaagaa tataacacca gcaatcctga cttgttgcta
      601 gtcatatttc aagtgacagg catcagcctc ctgccaccac tgggagttgc catatctgtc
      661 atcatcatct tctactgcta ccgcgttaac cggcagcaga agctgagttc aacctgggaa
      721 accggcaaga cgcggaagct catggagttc agcgagcact gtgccatcat cctggaagat
      781 gaccgctctg acatcagctc cacgtgtgcc aacaacatca accacaacac agagctgctg
      841 cccattgagc tggacaccct ggtggggaaa ggtcgctttg ctgaggtcta taaggccaag
      901 ctgaagcaga acacttcaga gcagtttgag acagtggcag tcaagatctt tccctatgag
      961 gagtatgcct cttggaagac agagaaggac atcttctcag acatcaatct gaagcatgag
     1021 aacatactcc agttcctgac ggctgaggag cggaagacgg agttggggaa acaatactgg
     1081 ctgatcaccg ccttccacgc caagggcaac ctacaggagt acctgacgcg gcatgtcatc
     1141 agctgggagg acctgcgcaa gctgggcagc tccctcgccc gggggattgc taacctccac
     1201 agtgatcaca ctccatgtgg gaggcccaag atgcccatcg tgcacaggga cctcaagagc
     1261 tccaatatcc tcgtgaagaa cgacctaacc tgctgcctgt gtgactttgg gctttccctg
     1321 cgtctggacc ctactctgtc tgtggatgac ctggctaaca gtgggcaggt gggaactgca
     1381 agatacatgg ctccagaagt cctagaatcc aggatgaatt tggagaatgt tgagtccttc
     1441 aagcagaccg atgtctactc catggctctg gtgctctggg aaatgacatc tcgctgtaat
     1501 gcagtgggag aagtaaaaga ttatgagcct ccatttggtt ccaaggtgcg ggagcacccc
     1561 tgtgtcgaaa gcatgaagga caacgtgttg agagatcgag ggcgaccaga aattcccagc
     1621 ttctggctca accaccaggg catccagatg gtgtgtgaga cgttgactga gtgctgggac
     1681 cacgacccag aggcccgtct cacagcccag tgtgtggcag aacgcttcag tgagctggag
     1741 catctggaca ggctctcggg gaggagctgc tcggaggaga agattcctga agacggctcc
     1801 ctaaacacta ccaaaagcag cggtggagca agcgcagcca gtggtagcgc ggatccaccg
     1861 gtcgccacca tgagtgcgat taagccagac atgaagatca aactccgtat ggaaggcaac
     1921 gtaaacgggc accactttgt gatcgacgga gatggtacag gcaagccttt tgagggaaaa
     1981 cagagtatgg atcttgaagt caaagagggc ggacctctgc cttttgcctt tgatatcctg
     2041 accactgcat tccattacgg caacagggta ttcgccaaat atccagacaa catacaagac
     2101 tattttaagc agtcgtttcc taaggggtat tcgtgggaac gaagcttgac tttcgaagac
     2161 gggggcattt gcattgccag aaacgacata acaatggaag gggacacttt ctataataaa
     2221 gttcgatttt atggtaccaa ctttcccgcc aatggtccag ttatgcagaa gaagacgctg
     2281 aaatgggagc cctccactga gaaaatgtat gtgcgtgatg gagtgctgac gggtgatatt
     2341 catatggctt tgttgcttga aggaaatgcc cattaccgat gtgacttcag aactacttac
     2401 aaagctaagg agaagggtgt caagttacca ggctaccact ttgtggacca ctgcattgag
     2461 attttaagcc atgacaaaga ttacaacaag gttaagctgt atgagcatgc tgttgctcat
     2521 tctggattgc ctgacaatgc cagacgataa gcggccgcga ctctagatca taatcagcca
     2581 taccacattg tagaa
//
