LOCUS       sequence-88563-e        1209 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondrial Membrane, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1209)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos2-TOMM20-N-10
FEATURES             Location/Qualifiers
     source          1..1209
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             489..1169
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 acgtcagatc cgctagcgcc accatggtgg gtcggaacag cgccatcgcc gccggtgtat
       61 gcggggccct tttcattggg tactgcatct acttcgaccg caaaagacga agtgacccca
      121 acttcaagaa caggcttcga gaacgaagaa agaaacagaa gcttgccaag gagagagctg
      181 ggctttccaa gttacctgac cttaaagatg ctgaagctgt tcagaagttc ttccttgaag
      241 aaatacagct tggtgaagag ttactagctc aaggtgaata tgagaagggc gtagaccatc
      301 tgacaaatgc aattgctgtg tgtggacagc cacagcagtt actgcaggtc ttacagcaaa
      361 ctcttccacc accagtgttc cagatgcttc tgactaagct cccaacaatt agtcagagaa
      421 ttgtaagtgc tcagagcttg gctgaagatg atgtggaagg cggtagcggg gatccaccgg
      481 tcgccaccat gagtgcgatt aagccagaca tgaagatcaa actccgtatg gaaggcaacg
      541 taaacgggca ccactttgtg atcgacggag atggtacagg caagcctttt gagggaaaac
      601 agagtatgga tcttgaagtc aaagagggcg gacctctgcc ttttgccttt gatatcctga
      661 ccactgcatt ccattacggc aacagggtat tcgccaaata tccagacaac atacaagact
      721 attttaagca gtcgtttcct aaggggtatt cgtgggaacg aagcttgact ttcgaagacg
      781 ggggcatttg cattgccaga aacgacataa caatggaagg ggacactttc tataataaag
      841 ttcgatttta tggtaccaac tttcccgcca atggtccagt tatgcagaag aagacgctga
      901 aatgggagcc ctccactgag aaaatgtatg tgcgtgatgg agtgctgacg ggtgatattc
      961 atatggcttt gttgcttgaa ggaaatgccc attaccgatg tgacttcaga actacttaca
     1021 aagctaagga gaagggtgtc aagttaccag gctaccactt tgtggaccac tgcattgaga
     1081 ttttaagcca tgacaaagat tacaacaagg ttaagctgta tgagcatgct gttgctcatt
     1141 ctggattgcc tgacaatgcc agacgataag cggccgcgac tctagatcat aatcagccat
     1201 accacattt
//
