LOCUS       sequence-88564-e        1663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1663)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos2-Tropomyosin-N-14
FEATURES             Location/Qualifiers
     source          1..1663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             929..1609
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gccagctagt gacgtcagat ccgctagcgc caccatggac gccatcaaga agaagatgca
       61 gatgctgaag ctcgacaagg agaacgcctt ggatcgagct gagcaggcgg aggccgacaa
      121 gaaggcggcg gaagacagga gcaagcagct ggaagatgag ctggtgtcac tgcaaaagaa
      181 actcaagggc accgaagatg aactggacaa atactctgag gctctcaaag atgcccagga
      241 gaagctggag ctggcagaga aaaaggccac cgatgctgaa gccgacgtag cttctctgaa
      301 cagacgcatc cagctggttg aggaagagtt ggatcgtgcc caggagcgtc tggcaacagc
      361 tttgcagaag ctggaggaag ctgagaaggc agcagatgag agtgagagag gcatgaaagt
      421 cattgagagt cgagcccaaa aagatgaaga aaaaatggaa attcaggaga tccaactgaa
      481 agaggcaaag cacattgctg aagatgccga ccgcaaatat gaagaggtgg cccgtaagct
      541 ggtcatcatt gagagcgacc tggaacgtgc agaggagcgg gctgagctct cagaaggcaa
      601 atgtgccgag cttgaagaag aattgaaaac tgtgacgaac aacttgaagt cactggaggc
      661 tcaggctgag aagtactcgc agaaggaaga cagatatgag gaagagatca aggtcctttc
      721 cgacaagctg aaggaggctg agactcgggc tgagtttgcg gagaggtcag taactaaatt
      781 ggagaaaagc attgatgact tagaagacga gctgtacgct cagaaactga agtacaaagc
      841 catcagcgag gagctggacc acgctctcaa cgatatgact tccatatccg gcggtagcgg
      901 cggtagcggg gatccaccgg tcgccaccat gagtgcgatt aagccagaca tgaagatcaa
      961 actccgtatg gaaggcaacg taaacgggca ccactttgtg atcgacggag atggtacagg
     1021 caagcctttt gagggaaaac agagtatgga tcttgaagtc aaagagggcg gacctctgcc
     1081 ttttgccttt gatatcctga ccactgcatt ccattacggc aacagggtat tcgccaaata
     1141 tccagacaac atacaagact attttaagca gtcgtttcct aaggggtatt cgtgggaacg
     1201 aagcttgact ttcgaagacg ggggcatttg cattgccaga aacgacataa caatggaagg
     1261 ggacactttc tataataaag ttcgatttta tggtaccaac tttcccgcca atggtccagt
     1321 tatgcagaag aagacgctga aatgggagcc ctccactgag aaaatgtatg tgcgtgatgg
     1381 agtgctgacg ggtgatattc atatggcttt gttgcttgaa ggaaatgccc attaccgatg
     1441 tgacttcaga actacttaca aagctaagga gaagggtgtc aagttaccag gctaccactt
     1501 tgtggaccac tgcattgaga ttttaagcca tgacaaagat tacaacaagg ttaagctgta
     1561 tgagcatgct gttgctcatt ctggattgcc tgacaatgcc agacgataag cggccgcgac
     1621 tcttagatca taatcagcca taccacattg tagaagattt gcc
//
