LOCUS       sequence-88572-e        3975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: mEos2-Vinculin-14
FEATURES             Location/Qualifiers
     source          1..3975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..692
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gatccgctag caccatgagt gcgattaagc cagacatgaa gatcaaactc cgtatggaag
       61 gcaacgtaaa cgggcaccac tttgtgatcg acggagatgg tacaggcaag ccttttgagg
      121 gaaaacagag tatggatctt gaagtcaaag agggcggacc tctgcctttt gcctttgata
      181 tcctgaccac tgcattccat tacggcaaca gggtattcgc caaatatcca gacaacatac
      241 aagactattt taagcagtcg tttcctaagg ggtattcgtg ggaacgaagc ttgactttcg
      301 aagacggggg catttgcatt gccagaaacg acataacaat ggaaggggac actttctata
      361 ataaagttcg attttatggt accaactttc ccgccaatgg tccagttatg cagaagaaga
      421 cgctgaaatg ggagccctcc actgagaaaa tgtatgtgcg tgatggagtg ctgacgggtg
      481 atattcatat ggctttgttg cttgaaggaa atgcccatta ccgatgtgac ttcagaacta
      541 cttacaaagc taaggagaag ggtgtcaagt taccaggcta ccactttgtg gaccactgca
      601 ttgagatttt aagccatgac aaagattaca acaaggttaa gctgtatgag catgctgttg
      661 ctcattctgg attgcctgac aatgccagac gagagaattc gggaaactcg agaacaaagc
      721 ttgaaaacgg ccgcatgcca gtgtttcata cgcgcacgat cgagagcatc ctggagccgg
      781 tggcacagca gatctcccac ctggtgataa tgcacgagga gggcgaggtg gacggcaaag
      841 ccattcctga cctcaccgcg cccgtggccg ccgtgcaggc ggccgtcagc aacctcgtcc
      901 gggttggaaa agagactgtt caaaccactg aggatcagat tttgaagaga gatatgccac
      961 cagcatttat taaggttgag aatgcttgca ccaagcttgt ccaggcagct cagatgcttc
     1021 agtcagaccc ttactcagtg cctgctcgag attatctaat tgatgggtca aggggcatcc
     1081 tctctggaac atcagacctg ctccttacct tcgatgaggc tgaggtccgt aaaattatta
     1141 gagtttgcaa aggaattttg gaatatctta cagtggcaga ggtggtggag actatggaag
     1201 atttggtcac ttacacaaag aatcttgggc caggaatgac taagatggcc aagatgattg
     1261 acgagagaca gcaggagctc actcaccagg agcaccgagt gatgttggtg aactcgatga
     1321 acaccgtgaa agagttgctg ccagttctca tttcagctat gaagattttt gtaacaacta
     1381 aaaactcaaa aaaccaaggc atagaggaag ctttaaaaaa tcgcaatttt actgtagaaa
     1441 aaatgagtgc tgaaattaat gagataattc gtgtgttaca actcacctct tgggatgaag
     1501 atgcctgggc cagcaaggac actgaagcca tgaagagagc attggcctcc atagactcca
     1561 aactgaacca ggccaaaggt tggctccgtg accctagtgc ctccccaggg gatgctggtg
     1621 agcaggccat cagacagatc ttagatgaag ctggaaaagt tggtgaactc tgtgcaggca
     1681 aagaacgcag ggagattctg ggaacttgca aaatgctagg gcagatgact gatcaagtgg
     1741 ctgacctccg tgccagagga caaggatcct caccggtggc catgcagaaa gctcagcagg
     1801 tatctcaggg tctggatgtg ctcacagcaa aagtggaaaa tgcagctcgc aagctggaag
     1861 ccatgaccaa ctcaaagcag agcattgcaa agaagatcga tgctgctcag aactggcttg
     1921 cagatccaaa tggtggaccg gaaggagaag agcagattcg aggtgctttg gctgaagctc
     1981 ggaaaatagc agaattatgt gatgatccta aagaaagaga tgacattcta cgttcccttg
     2041 gggaaatatc tgctctgact tctaaattag cagatctacg aagacagggg aaaggagatt
     2101 ctccagaggc tcgagccttg gccaaacagg tggccacggc cctgcagaac ctgcagacca
     2161 aaaccaaccg ggctgtggcc aacagcagac cggccaaagc agctgtacac cttgagggca
     2221 agattgagca agcacagcgg tggattgata atcccacagt ggatgaccgt ggagtcggtc
     2281 aggctgccat ccgggggctt gtggccgaag ggcatcgtct ggctaatgtt atgatggggc
     2341 cttatcggca agatcttctc gccaagtgtg accgagtgga ccagctgaca gcccagctgg
     2401 ctgacctggc tgccagaggg gaaggggaga gtcctcaggc acgagcactt gcatctcagc
     2461 tccaagactc cttaaaggat ctaaaagctc ggatgcagga ggccatgact caggaagtgt
     2521 cagatgtttt cagcgatacc acaactccca tcaagctgtt ggcagtggca gccacggcgc
     2581 ctcctgatgc gcctaacagg gaagaggtat ttgatgagag ggcagctaac tttgaaaacc
     2641 attcaggaaa gcttggtgct acggccgaga aggcggctgc ggttggtact gctaataaat
     2701 caacagtgga aggcattcag gcctcagtga agacggcccg agaactcaca ccccaggtgg
     2761 tctcggctgc tcgtatctta cttaggaacc ctggaaatca agctgcttat gaacattttg
     2821 agaccatgaa gaaccagtgg atcgataatg ttgaaaaaat gacagggctg gtggacgaag
     2881 ccattgatac caaatctctg ttggatgctt cagaagaagc aattaaaaaa gacctggaca
     2941 agtgcaaggt agctatggcc aacattcagc ctcagatgct ggttgctggg gcaaccagta
     3001 ttgctcgtcg ggccaaccgg atcctgctgg tggctaagag ggaggtggag aattccgagg
     3061 atcccaagtt ccgtgaggct gtgaaagctg cctctgatga attgagcaaa accatctccc
     3121 cgatggtgat ggatgcaaaa gctgtggctg gaaacatttc cgaccctgga ctgcaaaaga
     3181 gcttcctgga ctcaggatat cggatcctgg gagctgtggc caaggtcaga gaagccttcc
     3241 aacctcagga gcctgacttc ccgccgcctc caccagacct tgaacaactc cgactaacag
     3301 atgagcttgc tcctcccaaa ccacctctgc ctgaaggtga ggtccctcca cctaggcctc
     3361 caccaccaga ggaaaaggat gaagagttcc ctgagcagaa ggccggggag gtgattaacc
     3421 agccaatgat gatggctgcc agacagctcc atgatgaagc tcgcaaatgg tccagcaagg
     3481 gcaatgacat cattgcagca gccaagcgca tggctctgct gatggctgag atgtctcggc
     3541 tggtaagagg gggcagtggt accaagcggg cactcattca gtgtgccaag gacatcgcca
     3601 aggcctcaga tgaggtgact cggttggcca aggaggttgc caagcagtgc acagataaac
     3661 ggattagaac caacctctta caggtatgtg agcgaatccc aaccataagc acccagctca
     3721 aaatcctgtc cacagtgaag gccaccatgc tgggccggac caacatcagt gatgaggagt
     3781 ctgagcaggc cacagagatg ctggttcaca atgcccagaa cctcatgcag tctgtgaagg
     3841 agactgtgcg ggaagctgaa gctgcttcaa tcaaaattcg aacagatgct ggatttacac
     3901 tgcgctgggt tagaaagact ccctggtacc agtaggcggc cgcgactcta gatcataatc
     3961 agccatacca cattt
//
