LOCUS       sequence-88574-e        6005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6005)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos2-ZO1-C-14
FEATURES             Location/Qualifiers
     source          1..6005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..711
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     RBS             2412..2420
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ccgtcagatc cgctagcgct accggtcgcc accatgagtg cgattaagcc agacatgaag
       61 atcaaactcc gtatggaagg caacgtaaac gggcaccact ttgtgatcga cggagatggt
      121 acaggcaagc cttttgaggg aaaacagagt atggatcttg aagtcaaaga gggcggacct
      181 ctgccttttg cctttgatat cctgaccact gcattccatt acggcaacag ggtattcgcc
      241 aaatatccag acaacataca agactatttt aagcagtcgt ttcctaaggg gtattcgtgg
      301 gaacgaagct tgactttcga agacgggggc atttgcattg ccagaaacga cataacaatg
      361 gaaggggaca ctttctataa taaagttcga ttttatggta ccaactttcc cgccaatggt
      421 ccagttatgc agaagaagac gctgaaatgg gagccctcca ctgagaaaat gtatgtgcgt
      481 gatggagtgc tgacgggtga tattcatatg gctttgttgc ttgaaggaaa tgcccattac
      541 cgatgtgact tcagaactac ttacaaagct aaggagaagg gtgtcaagtt accaggctac
      601 cactttgtgg accactgcat tgagatttta agccatgaca aagattacaa caaggttaag
      661 ctgtatgagc atgctgttgc tcattctgga ttgcctgaca atgccagacg atccggaagt
      721 gctgggagcg caagcggaag cggaggctcc ggtgaggaaa cagctatatg ggaacaacat
      781 acagtgacgc ttcacagggc tcctggattt ggatttggaa ttgcaatatc tggtggacga
      841 gataatcctc attttcagag tggggaaacg tcaatagtga tttcagatgt gctgaaagga
      901 ggaccagctg aaggacagct acaggaaaat gaccgagttg caatggttaa cggagtttca
      961 atggataatg ttgaacatgc ttttgctgtt cagcaactaa ggaaaagtgg gaaaaatgca
     1021 aaaattacaa ttagaaggaa gaagaaagtt caaataccag taagtcgtcc tgatcctgaa
     1081 ccagtatctg ataatgaaga agatagttat gatgaggaaa tacatgatcc aagaagtggc
     1141 cggagtggtg tggttaacag aaggagtgag aagatttggc cgagggatag aagtgcaagt
     1201 agagagagga gcttgtcccc gcggtcagac aggcggtcag tggcttccag ccagcctgct
     1261 aaacctacta aagtcacact ggtgaaatcc cggaaaaatg aagaatatgg tcttcgattg
     1321 gcaagccata tatttgttaa ggaaatttca caagatagtt tggcagcaag agatggcaat
     1381 attcaagaag gtgatgttgt attgaagata aatggtactg tgacagaaaa tatgtcattg
     1441 acagatgcaa agacattgat agaaaggtct aaaggcaaat taaaaatggt agttcaaaga
     1501 gatgaacggg ctacgctatt gaatgtccct gatctttctg acagcatcca ctctgctaat
     1561 gcctctgaga gagacgacat ttcagaaatt cagtcactgg catcagatca ttctggtcga
     1621 tcacacgata ggcctccccg ccgcagccgg tcacgatctc ctgaccagcg gtcagagcct
     1681 tctgatcatt ccaggcactc gccgcagcag ccaagcaatg gcagtctgcg gagtagagat
     1741 gaagagagaa tttctaaacc tggggctgtc tcaactcctg taaagcatgc tgatgatcac
     1801 acacctaaaa cagtggaaga agttacagtt gaaagaaatg agaaacaaac accttctctt
     1861 ccagaaccaa agcctgtgta tgcccaagtt ggcaaccaga tgtggattta cctgtcagtc
     1921 catctgatgg tgtcctacct aattcaactc atgaagatgg gatttcttcg gcccagcatg
     1981 aaattggtaa aattcagaaa aggagatagt gtgggtttgc ggctggctgg tggaaatgat
     2041 gttggaatat ttgtagctgg cgttctagaa gatagccctg cagccaagga aggcttagag
     2101 gaaggtgatc aaattctcag ggtaaacaac gtagatttta caaatatcat aagagaagaa
     2161 gccgtccttt tcctgcttga cctccctaaa ggagaagaag tgaccatatt ggctcagaag
     2221 aagaaggatg tttatcgtcg cattgtagaa tcagatgtag gagattcttt ctatattaga
     2281 acccattttg aatatgaaaa ggaatctccc tatggactta gttttaacaa aggagaggtg
     2341 ttccgtgctg tggatacctt gtacaatgga aaactgggct cttggcttgc tattcgaatt
     2401 ggtaaaaatc ataaggaggt agaacgaggc atcatcccta ataagaacag agctgagcag
     2461 ctcgccagtg tacagtatac acttccaaaa acagcaggcg gagaccgtgc tgacttctgg
     2521 agattcagag gtcttcgcag ctccaagaga aatcttcgaa aaagcagaga ggatttgtcc
     2581 gctcagcctg ttcaaacaaa gtttccagct tatgaaagag tggttcttcg agaagctgga
     2641 tttctgaggc ctgtaaccat ttttggacca atagctgatg ttgccagaga aaagctggca
     2701 agagaagaac cagatattta tcaaattgca aagagtgaac cacgagacgc tggaactgac
     2761 caacgtagct ctggctatat tcgcctgcat acaataaagc aaatcataga tcaagacaaa
     2821 catgctttat tagatgtaac accaaatgca gttgatcgtc ttaactatgc ccagtggtat
     2881 ccaattgttg tatttcttaa ccctgattct aagcaaggag taaaaacaat gagaatgagg
     2941 ttatgtccag aatctcggaa aagtgccagg aagttatacg agcgatctca taaacttgct
     3001 aaaaataatc accatctttt tacaactaca attaacttaa attcaatgaa tgatggttgg
     3061 tatggtgcgc tgaaagaagc agttcaacaa cagcaaaacc agctggtatg ggtttccgag
     3121 ggaaaggcgg atggtgctac aagtgatgac cttgatttgc atgatgatcg tctgtcctac
     3181 ctgtcagctc caggtagtga atactcaatg tatagcacgg acagtagaca cacttctgac
     3241 tatgaagaca cagacacaga aggcggggcc tacactgatc aagaactaga tgaaactctt
     3301 aatgatgagg ttgggactcc accggagtct gccattacac ggtcctctga gcctgtaaga
     3361 gaggactcct ctggaatgca tcatgaaaac caaacatatc ctccttactc accacaagcg
     3421 cagccacaac caattcatag aatagactcc cctggattta agccagcctc tcaacagaaa
     3481 gcagaagctt catctccagt cccttacctt tcgcctgaaa caaacccagc atcatcaacc
     3541 tctgctgtta atcataatgt aaatttaact aatgtcagac tggaggagcc caccccagct
     3601 ccttccacct cttactcacc acaagctgat tctttaagaa caccaagtac tgaggcagct
     3661 cacataatgc taagagatca agaaccatca ttgtcgtcgc atgtagatcc aacaaaggtg
     3721 tatagaaagg acccatatcc cgaggaaatg atgaggcaga accatgtttt gaaacagcca
     3781 gccgttagtc acccagggca caggccagac aaagagccta atctgaccta tgaaccccaa
     3841 ctcccatacg tagagaaaca agccagcaga gacctggagc agcccacata cagatacgag
     3901 tcctcaagct atacggacca gttttctcga aactatgaac atcgtctgcg atacgaagat
     3961 cgcgtcccca tgtatgaaga acagtggtca tattatgatg acaaacagcc ctacccatct
     4021 cggccacctt ttgataatca gcactctcaa gaccttgact ccagacagca tcccgaagag
     4081 tcctcagaac gagggtactt tccacgtttt gaagagccag cccctctgtc ttacgacagc
     4141 agaccacgtt acgaacaggc acctagagca tccgccctgc ggcacgaaga gcagccagct
     4201 cctgggtatg acacacatgg tagactcaga ccggaagccc agccccaccc ttcagcaggg
     4261 cccaagcctg cagagtccaa gcagtatttt gagcaatatt cacgcagtta cgagcaagta
     4321 ccaccccaag gatttacctc tagagcaggt cattttgagc ctctccatgg tgctgcagct
     4381 gtccctccgc tgataccttc atctcagcat aagccagaag ctctgccttc aaacaccaaa
     4441 ccactgcctc cacccccaac tcaaaccgaa gaagaggaag atccagcaat gaagccacag
     4501 tctgtactca ccagagttaa gatgtttgaa aacaaaaggt ctgcatcctt agagaccaag
     4561 aaggatgtaa atgacactgg cagttttaag cctccagaag tagcatctaa accttcaggt
     4621 gctcccatca ttggtcccaa acccacttct cagaatcaat tcagtgaaca tgacaaaact
     4681 ctgtacagga ttccagaacc tcaaaaacct caactgaagc cacctgaaga tattgttcgg
     4741 tccaatcatt atgaccctga agaagatgaa gaatattatc gaaaacagct gtcatacttt
     4801 gaccgaagaa gttttgagaa taagcctcct gcacacattg ctgccagcca tctctccgag
     4861 cctgcaaagc cagctcattc tcagaatcaa tcaaattttt ctagttattc ttcaaaggga
     4921 aagcctcctg aagctgatgg tgtggataga tcatttggcg agaaacgcta tgaacccatc
     4981 caggccactc cccctcctcc tccattgccc tcgcagtatg cccagccatc tcagcctgtc
     5041 accagcgcgt ctctccacat acattctaag ggagcacatg gtgaaggtaa ttcagtgtca
     5101 ttggattttc agaattcctt agtgtccaaa ccagacccac ctccatctca gaataagcca
     5161 gcaactttca gaccaccaaa ccgagaagat actgctcagg cagctttcta tccccagaaa
     5221 agttttccag ataaagcccc agttaatgga actgaacaga ctcagaaaac agtcactcca
     5281 gcatacaatc gattcacacc aaaaccatat acaagttctg cccgaccatt tgaacgcaag
     5341 tttgaaagtc ctaaattcaa tcacaatctt ctgccaagtg aaactgcaca taaacctgac
     5401 ttgtcttcaa aaactcccac ttctccaaaa actcttgtga aatcgcacag tttggcacag
     5461 cctcctgagt ttgacagtgg agttgaaact ttctctatcc atgcagagaa gcctaaatat
     5521 caaataaata atatcagcac agtgcctaaa gctattcctg tgagtccttc agctgtggaa
     5581 gaggatgaag atgaagatgg tcatactgtg gtggccacag cccgaggcat atttaacagc
     5641 aatgggggcg tgctgagttc catagaaact ggtgttagta taattatccc tcaaggagcc
     5701 attcccgaag gagttgagca ggaaatctat ttcaaggtct gccgggacaa cagcatcctt
     5761 ccacctttag ataaagagaa aggtgaaaca ctgctgagtc ctttggtgat gtgtggtccc
     5821 catggcctca agttcctgaa gcctgtggag ctgcgcttac cacactgtga tcctaaaacc
     5881 tggcaaaaca agtgtcttcc cggagatcca aattatctcg ttggagcaaa ctgtgtttct
     5941 gtccttattg accactttta aggatccacc ggatctagat aactgatcat aatcagccat
     6001 accac
//
