LOCUS       sequence-88576-e        2547 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2547)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos2-Zyxin-C-14
FEATURES             Location/Qualifiers
     source          1..2547
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..714
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     regulatory      749..758
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtgacgtcag atccgctagc gctaccggtc gccaccatga gtgcgattaa gccagacatg
       61 aagatcaaac tccgtatgga aggcaacgta aacgggcacc actttgtgat cgacggagat
      121 ggtacaggca agccttttga gggaaaacag agtatggatc ttgaagtcaa agagggcgga
      181 cctctgcctt ttgcctttga tatcctgacc actgcattcc attacggcaa cagggtattc
      241 gccaaatatc cagacaacat acaagactat tttaagcagt cgtttcctaa ggggtattcg
      301 tgggaacgaa gcttgacttt cgaagacggg ggcatttgca ttgccagaaa cgacataaca
      361 atggaagggg acactttcta taataaagtt cgattttatg gtaccaactt tcccgccaat
      421 ggtccagtta tgcagaagaa gacgctgaaa tgggagccct ccactgagaa aatgtatgtg
      481 cgtgatggag tgctgacggg tgatattcat atggctttgt tgcttgaagg aaatgcccat
      541 taccgatgtg acttcagaac tacttacaaa gctaaggaga agggtgtcaa gttaccaggc
      601 taccactttg tggaccactg cattgagatt ttaagccatg acaaagatta caacaaggtt
      661 aagctgtatg agcatgctgt tgctcattct ggattgcctg acaatgccag acgatccgga
      721 ctcagatctc gagctcaagc ttcgaattcc atggcggccc cccgcccgtc tcccgcgatc
      781 cccgtttcgg tctcggctcc ggctttttac gccccgcaga agaagttcgg ccctgtggtg
      841 gccccaaagc ccaaagtgaa tcccttccgg cccagggaca gcgagcctcc cccggcaccc
      901 ggggcccagc gcgcacagat gggccgggtg ggcgatattc ccccgccgcc cccggaagac
      961 tttcccctgc ctccacctcc ccttgctggg gatggcgacg atgcagaggg tgctctggga
     1021 ggtgccttcc cgccgccccc tcccccgatc gaggaatcat ttccccctgc gcctctggag
     1081 gaggagatct tcccttcccc gccgcctcct ccggaggagg agggagggcc tgaggccccc
     1141 ataccgcccc caccacagcc cagggagaag gtgagcagta ttgatttgga gatcgactct
     1201 ctgtcctcac tgctggatga catgaccaag aatgatcctt tcaaagcccg ggtgtcatct
     1261 ggatatgtgc ccccaccagt ggccactcca ttcagttcca agtccagtac caagcctgca
     1321 gccgggggca cagcacccct gcctccttgg aagtcccctt ccagctccca gcctctgccc
     1381 caggttccgg ctccggctca gagccagaca cagttccatg ttcagcccca gccccagccc
     1441 aagcctcagg tccaactcca tgtccagtcc cagacccagc ctgtgtcttt ggctaacacc
     1501 cagccccgag ggcccccagc ctcatctccg gctccagccc ctaagttttc tccagtgact
     1561 cctaagttta ctcctgtggc ttccaagttc agtcctggag ccccaggtgg atctgggtca
     1621 caaccaaatc aaaaattggg gcaccccgaa gctctttctg ctggcacagg ctcccctcaa
     1681 cctcccagct tcacctatgc ccagcagagg gagaagcccc gagtgcagga gaagcagcac
     1741 cccgtgcccc caccggctca gaaccaaaac caggtgcgct cccctggggc cccagggccc
     1801 ctgactctga aggaggtgga ggagctggag cagctgaccc agcagctaat gcaggacatg
     1861 gagcatcctc agaggcagaa tgtggctgtc aacgaactct gcggccgatg ccatcaaccc
     1921 ctggcccggg cgcagccagc cgtccgcgct ctagggcagc tgttccacat cgcctgcttc
     1981 acctgccacc agtgtgcgca gcagctccag ggccagcagt tctacagtct ggagggggcg
     2041 ccgtactgcg agggctgtta cactgacacc ctggagaagt gtaacacctg cggggagccc
     2101 atcactgacc gcatgctgag ggccacgggc aaggcctatc acccgcactg cttcacctgt
     2161 gtggtctgcg cccgccccct ggagggcacc tccttcatcg tggaccaggc caaccggccc
     2221 cactgtgtcc ccgactacca caagcagtac gccccgaggt gctccgtctg ctctgagccc
     2281 atcatgcctg agcctggccg agatgagact gtgcgagtgg tcgccctgga caagaacttc
     2341 cacatgaagt gttacaagtg tgaggactgc gggaagcccc tgtcgattga ggcagatgac
     2401 aatggctgct tccccctgga cggtcacgtg ctctgtcgga agtgccacac tgctagagcc
     2461 cagacctaag aattctgcag tcgacggtac cgcgggcccg ggatccaccg gatctagata
     2521 actgatcata atcagccata ccacatt
//
