LOCUS       sequence-88584-e        1310 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 507 / 572, Emission: 
            516 / 580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1310)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos3.2-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..1310
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    530..582
                     /label=MCS
                     /note="multiple cloning site"
     CDS             596..1276
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gacgtcagat ccgctagcca ccatgggccc caagcggcgg cagctgacgt tccgggagaa
       61 gtcgcggatc atccaggagg tggaggagaa cccggaccta cgcaagggcg agatcgcgcg
      121 gcgcttcaac atcccgccgt ccacgctgag cacgatcctg aagaacaagc gcgccatcct
      181 ggcgtcggag cgcaagtacg gggtggcctc cacctgccgc aagaccaaca agctgtctcc
      241 ctacgacaag ctcgagggct tgctcatcgc ctggttccag cagatccgcg ccgccggcct
      301 gccggtcaag ggcatcatcc tcaaggagaa ggcgctgcgc atagccgagg agctgggcat
      361 ggacgacttc accgcctcca acggctggct ggaccgcttc cgccggcgcc acggcgtggt
      421 gtcctgcagc ggcgtggccc gcgcccgcgc gcgaaacgct gccccccgca ccccggcggc
      481 gcctgccagt ccggccgcgg tgccctcgga gggcagtggc gggagcactc tcgagctcaa
      541 gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggtcg ccaccatgag
      601 tgcgattaag ccagacatga agatcaaact ccgtatggaa ggcaacgtaa acgggcacca
      661 ctttgtgatc gacggagatg gtacaggcaa gccttttgag ggaaaacaga gtatggatct
      721 tgaagtcaaa gagggcggac ctctgccttt tgcctttgat atcctgacca ctgcattcca
      781 ttacggcaac agggtattcg ccaaatatcc agacaacata caagactatt ttaagcagtc
      841 gtttcctaag gggtattcgt gggaacgaag cttgactttc gaagacgggg gcatttgcaa
      901 cgccagaaac gacataacaa tggaagggga cactttctat aataaagttc gattttatgg
      961 taccaacttt cccgccaatg gtccagttat gcagaagaag acgctgaaat gggagccctc
     1021 cactgagaaa atgtatgtgc gtgatggagt gctgacgggt gatattgaga tggctttgtt
     1081 gcttgaagga aatgcccatt accgatgtga cttcagaact acttacaaag ctaaggagaa
     1141 gggtgtcaag ttaccaggcg cccactttgt ggaccactgc attgagattt taagccatga
     1201 caaagattac aacaaggtta agctgtatga gcatgctgtt gctcattctg gattgcctga
     1261 caatgccaga cgataagcgg ccgcgactct agatcataat cagccatacc
//
