LOCUS       sequence-88587-e        1644 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 507 / 572, 
            Emission: 516 / 580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Huang F, Hartwich TM, Rivera-Molina FE, Lin Y, Duim WC, Long JJ, 
            Uchil PD, Myers JR, Baird MA, Mothes W, Davidson MW, Toomre D, 
            Bewersdorf J
  TITLE     Video-rate nanoscopy using sCMOS camera-specific single-molecule 
            localization algorithms.
  JOURNAL   Nat Methods. 2013 Jul;10(7):653-8. doi: 10.1038/nmeth.2488. Epub 
            2013 May 26.
  PUBMED    23708387
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2488"; journalName: "Nat Methods"; date: "2013-07"; 
            volume: "10"; issue: "7"; pages: "653-8"
COMMENT     Sequence Label: mEos3.2-EB3-7
FEATURES             Location/Qualifiers
     source          1..1644
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      57..66
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             927..1607
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 cgctagcgct accggactca gatctcgagc tcaagcttcg aattctgcag tcgactgcca
       61 ccatggccgt caatgtgtac tccacatctg tgaccagtga aaatctgagt cgccatgata
      121 tgcttgcatg ggtcaacgac tccctgcacc tcaactatac caagatagaa cagctttgtt
      181 caggggcagc ctactgccag ttcatggaca tgctcttccc cggctgtgtg cacttgagga
      241 aagtgaagtt ccaggccaaa ctagagcatg aatacatcca caacttcaag gtgctgcaag
      301 cagctttcaa gaagatgggt gttgacaaaa tcattcctgt agagaaatta gtgaaaggaa
      361 aattccaaga taattttgag tttattcagt ggtttaagaa attctttgac gcaaactatg
      421 atggaaagga ttacaaccct ctgctggcgc ggcagggcca ggacgtagcg ccacctccta
      481 acccaggtga tcagatcttc aacaaatcca agaaactcat tggcacagca gttccacaga
      541 ggacgtcccc cacaggccca aaaaacatgc agacctctgg ccggctgagc aatgtggccc
      601 ccccctgcat tctccggaag aatcctccat cagcccgaaa tggcggccat gagactgatg
      661 cccaaattct tgaactcaac caacagctgg tggacttgaa gctgacagtg gatgggctgg
      721 agaaggaacg tgacttctac ttcagcaaac ttcgtgacat cgagctcatc tgccaggagc
      781 atgaaagtga aaacagccct gttatctcag gcatcattgg catcctctat gccacagagg
      841 aaggattcgc accccctgag gacgatgaga ttgaagagca tcaacaagaa gaccaggacg
      901 agtacctgga tccaccggtc gccaccatga gtgcgattaa gccagacatg aagatcaaac
      961 tccgtatgga aggcaacgta aacgggcacc actttgtgat cgacggagat ggtacaggca
     1021 agccttttga gggaaaacag agtatggatc ttgaagtcaa agagggcgga cctctgcctt
     1081 ttgcctttga tatcctgacc actgcattcc attacggcaa cagggtattc gccaaatatc
     1141 cagacaacat acaagactat tttaagcagt cgtttcctaa ggggtattcg tgggaacgaa
     1201 gcttgacttt cgaagacggg ggcatttgca acgccagaaa cgacataaca atggaagggg
     1261 acactttcta taataaagtt cgattttatg gtaccaactt tcccgccaat ggtccagtta
     1321 tgcagaagaa gacgctgaaa tgggagccct ccactgagaa aatgtatgtg cgtgatggag
     1381 tgctgacggg tgatattgag atggctttgt tgcttgaagg aaatgcccat taccgatgtg
     1441 acttcagaac tacttacaaa gctaaggaga agggtgtcaa gttaccaggc gcccactttg
     1501 tggaccactg cattgagatt ttaagccatg acaaagatta caacaaggtt aagctgtatg
     1561 agcatgctgt tgctcattct ggattgcctg acaatgccag acgataagcg gccgcgactc
     1621 tagatcataa tcagccatac caca
//
