LOCUS       sequence-8859-ex        6769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Wang H, Heinz ME, Crosby SD, Johnston M, Mitra RD
  TITLE     'Calling Cards' method for high-throughput identification of targets
            of yeast DNA-binding proteins.
  JOURNAL   Nat Protoc. 2008;3(10):1569-77. doi: 10.1038/nprot.2008.148.
  PUBMED    18802438
REFERENCE   2  (bases 1 to 6769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2008"; volume: "3(10):1569-77. doi"; pages: " 
            10.1038/nprot.2008.148"
COMMENT     Sequence Label: pBM5218
FEATURES             Location/Qualifiers
     source          1..6769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    87..204
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     5385..5401
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5386..5402
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6208..6395)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(6745..6761)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6745..6761)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ctcgagcccc attatcttag cctaaaaaaa ccttctcttt ggaactttca gtaatacgct
       61 taactgctca ttgctatatt gaagtacgga ttagaagccg ccgagcgggt gacagccctc
      121 cgaaggaaga ctctcctccg tgcgtcctcg tcttcaccgg tcgcgttcct gaaacgcaga
      181 tgtgcctcgc gccgcactgc tccgaacaat aaagattcta caatactagc ttttatggtt
      241 atgaagagga aaaattggca gtaacctggc cccacaaacc ttcaaatgaa cgaatcaaat
      301 taacaaccat aggatgaggg taatgtttcg tactgtttca ggaattgttt cagtaatgtt
      361 ttagacaagg aaaacataga gcagcaaacc tccgatccga cagtacttaa gaaaccatag
      421 tttctgtgta caagagtagt acctatgtaa ttcttacatt tacataacat atagaaaggt
      481 ccaataaact tacaacatta tgacatataa gctagatcgt aattcactac gtcaacaggt
      541 tatgagccct gagagcaatg cttcagagac cataattaat ctatctaatc ccaacaatta
      601 taaacagtgg ctgtacggta tcgagaccgc tgctgaatat gctaacgaat atatgaacga
      661 attcgttcat accggagata tccaatcaat gaaaagggat tgcaatctca gcgcgaatga
      721 tgaaagcttt gtcaaaaccg tatttaacag tttcctggta aagctctaca agaaaactat
      781 cgtgggtgaa gctgcatgtg aaatgaactg gatatgtgat gattcacttg gaagggtctc
      841 tgcttatgat attttctcgc acttcgaaga aaactattat gaagtcacta gtggatccag
      901 gcttactctt atagaggact taccaaatat atcctccaag cctgtagatg aaatcgcttc
      961 ctttttgaaa accctattca caatgcttga agacaatagc gaagaacagg acaaaaagaa
     1021 aagacgcgat accaatatcg cgttgctatt aatgaccttc ttacccgagt taaaggaatc
     1081 attccacgag aaattcggtg actctaaagc tcttcagctg tcacaagtca ttagattctg
     1141 taaattaaag gcgtcatcga attcattatc ttcagtctca gatgcattgg ttgcacaaga
     1201 cagaagaagc tatcaaaaga aaggaaataa gggatgtatg atttgtgggg ctaatcatcg
     1261 cttaagcaac tgttctctgc ttaaaagaag aataccagaa gccagaatct ttaaattata
     1321 tcctaatgac aagacgaata gatcttcatc tgctagtgtt gcgattcctg actatgaaac
     1381 gcaaggccaa acagcaggac agataacacc aaagtcctgg ctctgtatgt tatcttcgac
     1441 cgtcccagct accaaatcct cagattggat ttgtgacaca ggatgtactt cacacatgtg
     1501 ccacgaccgt tctatgttct catcatttac tagatcctct aagaaagact ttgtcagagg
     1561 agtcggcggt tccataccca tcatgggctc cgggactgta aacatcggca ctgttcaatt
     1621 aaatgacgta tcctacgtcc ctgatttacc agtcaaccta atatccattt ggaaactatg
     1681 tgctaaatcc aactcttctg ttacgttcac aaaagagggt gtcactgtga aatcacctga
     1741 tgacgtgatt tctacggctg ggaagttaaa caattatctg tacattttcg atgatcttac
     1801 gcccgtaact accttctctt cgcaaaatta cttctgctct aaaacattgg attcatctaa
     1861 aatgataact tccgcagcgt ttcataccgt tgcagataaa atgttgtcgc aacacatttc
     1921 tcccactgct ctcccggtaa aatggcatgc tcgtatgggc catcccggag cagatattta
     1981 caattccttg gctagaactc tgcgttttcc aaaatttaag acggctgaat acactatttg
     2041 tcctacctgc tcactagcaa aaggaatcat caaaaagggt aaagtctcgc tcaaaaaata
     2101 tacccaacct cttcaaatgg tacaggctga tctctgtggt gggtttcgct accaagagtt
     2161 tcagtcaaat aaatattttc ttactatccg tgatgcctat agtcgctact actctgtaat
     2221 acatttaaaa tccaaagcag acgctccgat aaaattcatg gaatggatca acgaaaccga
     2281 acaatacttt agctcccggg gtggattcaa agtcggatct gttcgtacag acaatggtac
     2341 agaattcgta aataaaaatc ttcatgcgtt ttttaaatct aaaggaatag agcatcagtt
     2401 aactattcca tatcatagtt atcaaaatgg tgctgttgaa cgtgcacatc gtaccatcga
     2461 agaacgcact cgttgtctcc ttatcggggg gcgtgttcct ccgtccttgt ggtctgaagc
     2521 tgtttcttgc gcagtctatt taatcaatag gtcccctgta gtgtccaaaa ataacagtat
     2581 cccatactgc cggtggttca acatccccgc aaaagatttc ggtatcgcac atcttcgaat
     2641 ttttggatgt acagcatacg caaccttaca acctagtctt cgagacggca aacttgcccc
     2701 aactgtcata tctggtgtta tggttggcta tgactctaac catcgaggat acaggattta
     2761 tcatcccgaa actggccgca tctttgtgag cagtcaagtt cgatttgacg aacacatgtt
     2821 tcctcttgct gatacagagg cagttcacgt ctctcacgac tttgccactt ccgctattgg
     2881 gggggtgtcc aaatatcctg aaacagggtc aaccgtctct gctccaaaga acgacggatc
     2941 tgacttggca aatttgccaa taactgttcc caaaaatgta aatcaaccag cacataaacc
     3001 taataccagt aacatctctt cctctgatga tgatgaggat atttcaatgg aaatcgaaat
     3061 ggaaaaacct atccctgagt gtaaccaaga caacttacca aactccggat gtccaccaac
     3121 aaggatacaa cattctaact ttgaatcctt accaaccgtg tctaccgaag acgaaactaa
     3181 ttcttctatg gagaaaactc ctgaaagagt tccagcggca ctaacttatc gagaaattcc
     3241 aaaatcatcc gattcagaat atattccgac atgccgaaat agaactagac gtgttaaaag
     3301 aactaataag aaaccaacgc gatcccgcga aatagaaata tatgatatat cacgtccaaa
     3361 cgtaatatcg agtgacaact tacctgaagt tagaagtgcc aagcaaagaa agacggtgtc
     3421 caatacaaat gatactgtag caaggacaaa tagacttcca accgtgctac gaactctaga
     3481 ctcaaacaac attgacacgc tgcatgttgc cagtactggt gaagaagtgt ccatcgaaag
     3541 actttcaagc atggctcttc aggaagcgaa gaacaattcc gccagaacta atcaagctaa
     3601 ttctcttact gattggtttc cagtaggcgc aatgccgata cctgaccaga ggtatctatc
     3661 cgttcacgat ggaacatata tcagcgactc acaagatgtg ggtgatactg acctcactcc
     3721 tgctgtaacc aggctagtta ctgaagagaa ttcaatcgaa tctcctccat cgttggattc
     3781 atcgcctcca aatacctcat ttaacgcggc tctaactgct attatccata gcacaaaaaa
     3841 aggaaacccg aaaacctatg cccaagcaat gggaaggcct gactttcaag aatggcacaa
     3901 cgcatgcctc aaggaacttt ccgcgttcaa agatcacaat acgtacaaat tggtgtctct
     3961 tccaaagcaa agaagagctc ttggatcgcg ctgggtattc acaataaaag actccgggac
     4021 gtacaaagct cgccttgtcg cccaaggaca tactcaaaag gctggtattg actatcaaga
     4081 aacttttgca ccagtcattc gatatgactc tgttagatta tttctggccc ttgctagctg
     4141 cctcaaacta atagtatatc agatggacgt tgacaccgcg tttctaaact caaaaatgaa
     4201 tgagccggta tacgtaaaac aaccacccgg atttattaat gaaagtaatc ccgactatgt
     4261 atgggaacta tacggcggta tgtatggact caagcaagcc ccattactat ggaacgaaca
     4321 tatcaacaat actcttcaaa agattggttt tcgtcgacat gaaggcgaac atggcttata
     4381 ctttcgttcc acatctgatg gtcccatcta cattgcccta tacgtagacg acttacttgt
     4441 tgctgctccc tctccgaaaa tatatgacag ggttaagcag aaactaacga agttatactc
     4501 aatgaaggat ctaggtaaag ttgacaaatt cctcggtctt aacattaatc aattttcaaa
     4561 tggagacatc actctctcac ttcaagacta tattgctaaa gctgcatctg aaagcgaaat
     4621 aaacatatgt aagcctacac agactccgct ctgtgactca aagcctcttt tcgaaacaac
     4681 ttccccgcac ctaaaggaca tcactcctta tcagagcata gttggacagc ttctcttttg
     4741 tgcaaatact ggtcgtcctg acatatctta tccggtctca ctactctcca ggtttcttcg
     4801 cgaacctcgc gcaatccatt tggagtctgc tcgacgagtt ctacggtacc tatataccac
     4861 cagaagtatg tgtctcaagt atcgttctgg atctctgttg gcactaactg tatattgtga
     4921 tgcatctcat ggagcaattc acgatctccc acactctact ggggggtacg tgactctact
     4981 tgctggtgct ccagttacgt ggtcatcaaa gaaactcaag ggtgtgattc ctgtatcatc
     5041 tactgaggca gaatacatta ctgcaagtga aactgtcatg gagatagaat ggattcaaaa
     5101 cttgtttgaa cacttaggcc agccacttat ctcatcaaca ttatacgtag ataatgaacc
     5161 tgctataaaa ctatctaaac atcctgtatt tcacacgaga acaaaacaca ttgccttgag
     5221 atatcacaag ctaagaagtg cagtggcagc aggcataatt accatagagc atgttattac
     5281 aaagagacaa gttgctgaca tatttacaaa aatccttcca gcagaatcat tcaaagcaca
     5341 tagggctgtc atggtgaggg aaccagaaac tgcaaaataa cagatcgagg tcgacggtat
     5401 cgataagctt gggctgcagc tttaaataat cggtgtcact acataagaac acctttggtg
     5461 gagggaacat cgttggtacc attgggcgag gtggcttctc ttatggcaac cgcaagagcc
     5521 ttgaacgcac tctcactacg gtgatgatca ttcttgcctc gcagacaatc aacgtggagg
     5581 gtaattctgc tagcctctgc aaagctttca agaaaatgcg ggatcatctc gcaagagaga
     5641 tctcctactt tctccctttg caaaccaagt tcgacaactg cgtacggcct gttcgaaaga
     5701 tctaccaccg ctctggaaag tgcctcatcc aaaggcgcaa atcctgatcc aaaccttttt
     5761 actccacgca cggcccctag ggcctcttta aaagcttgac cgagagcaat cccgcagtct
     5821 tcagtggtgt gatggtcgtc tatgtgtaag tcaccaatgc actcaacgat tagcgaccag
     5881 ccggaatgct tgggtatgtt aatatggact aaaggaggct tttctgcagg tcgactctag
     5941 aggatccccc gggtaccgag ctcgaatttt tactaacaaa tggtattatt tattaacagc
     6001 cagagcatgt atcatatggt ccagaaaccc tatacctgtg tggacgttaa tcacttgcga
     6061 ttgtgtggcc tgttctgcta ctgcttctgc ctctttttct gggaagatcg agtgctctat
     6121 cgctagggga ccacccttta aagagatcgc aatctgaatc ttggtttcat ttgtaatacg
     6181 ctttactagg gctttctgct ctgtcatctt tgccttcgtt tatcttgcct gctcattttt
     6241 tagtatattc ttcgaagaaa tcacattact ttatataatg tataattcat tatgtgataa
     6301 tgccaatcgc taagaaaaaa aaagagtcat ccgctaggtg gaaaaaaaaa aatgaaaatc
     6361 attaccgagg cataaaaaaa tatagagtgt actagaggat cccgggcgag ctcgaattcc
     6421 gagcttatcg ataagcttga tatcgaattc ctgcagcccg ggggatctca tgcgtattca
     6481 gttatggggg gatgttgaat gtgataaccc aaaagcatga tatgggtaat gtttcagtac
     6541 tgtttcagaa ttgtttcagt aatgttttag acaaggaaaa catagagcag caaacctctg
     6601 atccgacagt acttaagaaa ccatagtttc tgtgtagaag agtagtacct atgtaattct
     6661 tacatttaca taacatatag aaaggtccaa taaacttaca acattatgac atataagcta
     6721 gatcgtaatt cactacgtca acaggatcca ctagttctag agcggccgc
//
