LOCUS       sequence-88601-e        1846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 507 / 572, Emission: 516 / 
            580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1846)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos3.2-MAPTau-N-10
FEATURES             Location/Qualifiers
     source          1..1846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1125..1805
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 atccgctagc gctaccggac tcagatctcg aggccaccat ggctgagccc cgccaggagt
       61 tcgaagtgat ggaagatcac gctgggacgt acgggttggg ggacaggaaa gatcaggggg
      121 gctacaccat gcaccaagac caagagggtg acacggacgc tggcctgaaa gctgaagaag
      181 caggcattgg agacaccccc agcctggaag acgaagctgc tggtcacgtg acccaagctc
      241 gcatggtcag taaaagcaaa gacgggactg gaagcgatga caaaaaagcc aagggggctg
      301 atggtaaaac gaagatcgcc acaccgcggg gagcagcccc tccaggccag aagggccagg
      361 ccaacgccac caggattcca gcaaaaaccc cgcccgctcc aaagacacca cccagctctg
      421 gtgaacctcc aaaatcaggg gatcgcagcg gctacagcag ccccggctcc ccaggcactc
      481 ccggcagccg ctcccgcacc ccgtcccttc caaccccacc cacccgggag cccaagaagg
      541 tggcagtggt ccgtactcca cccaagtcgc cgtcttccgc caagagccgc ctgcagacag
      601 cccccgtgcc catgccagac ctgaagaatg tcaagtccaa gatcggctcc actgagaacc
      661 tgaagcacca gccgggaggc gggaaggtgc aaatagtcta caaaccagtt gacctgagca
      721 aggtgacctc caagtgtggc tcattaggca acatccatca taaaccagga ggtggccagg
      781 tggaagtaaa atctgagaag cttgacttca aggacagagt ccagtcgaag attgggtccc
      841 tggacaatat cacccacgtc cctggcggag gaaataaaaa gattgaaacc cacaagctga
      901 ccttccgcga gaacgccaaa gccaagacag accacggggc ggagatcgtg tacaagtcgc
      961 cagtggtgtc tggggacacg tctccacggc atctcagcaa tgtctcctcc accggcagca
     1021 tcgacatggt agactcgccc cagctcgcca cgctagctga cgaggtgtct gcctccctgg
     1081 ccaagcaggg tttgagctcc ggaggggatc caccggtcgc caccatgagt gcgattaagc
     1141 cagacatgaa gatcaaactc cgtatggaag gcaacgtaaa cgggcaccac tttgtgatcg
     1201 acggagatgg tacaggcaag ccttttgagg gaaaacagag tatggatctt gaagtcaaag
     1261 agggcggacc tctgcctttt gcctttgata tcctgaccac tgcattccat tacggcaaca
     1321 gggtattcgc caaatatcca gacaacatac aagactattt taagcagtcg tttcctaagg
     1381 ggtattcgtg ggaacgaagc ttgactttcg aagacggggg catttgcaac gccagaaacg
     1441 acataacaat ggaaggggac actttctata ataaagttcg attttatggt accaactttc
     1501 ccgccaatgg tccagttatg cagaagaaga cgctgaaatg ggagccctcc actgagaaaa
     1561 tgtatgtgcg tgatggagtg ctgacgggtg atattgagat ggctttgttg cttgaaggaa
     1621 atgcccatta ccgatgtgac ttcagaacta cttacaaagc taaggagaag ggtgtcaagt
     1681 taccaggcgc ccactttgtg gaccactgca ttgagatttt aagccatgac aaagattaca
     1741 acaaggttaa gctgtatgag catgctgttg ctcattctgg attgcctgac aatgccagac
     1801 gataagcggc cgcgactcta gatcataatc agccatacca cattgt
//
