LOCUS       sequence-88608-e        6698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 507 / 572, Emission: 516 / 
            580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6698)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos3.2-MyosinIIA-C-18
FEATURES             Location/Qualifiers
     source          1..6698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..707
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 tcgagccgct agcgctaccg gtcgccacca tgagtgcgat taagccagac atgaagatca
       61 aactccgtat ggaaggcaac gtaaacgggc accactttgt gatcgacgga gatggtacag
      121 gcaagccttt tgagggaaaa cagagtatgg atcttgaagt caaagagggc ggacctctgc
      181 cttttgcctt tgatatcctg accactgcat tccattacgg caacagggta ttcgccaaat
      241 atccagacaa catacaagac tattttaagc agtcgtttcc taaggggtat tcgtgggaac
      301 gaagcttgac tttcgaagac gggggcattt gcaacgccag aaacgacata acaatggaag
      361 gggacacttt ctataataaa gttcgatttt atggtaccaa ctttcccgcc aatggtccag
      421 ttatgcagaa gaagacgctg aaatgggagc cctccactga gaaaatgtat gtgcgtgatg
      481 gagtgctgac gggtgatatt gagatggctt tgttgcttga aggaaatgcc cattaccgat
      541 gtgacttcag aactacttac aaagctaagg agaagggtgt caagttacca ggcgcccact
      601 ttgtggacca ctgcattgag attttaagcc atgacaaaga ttacaacaag gttaagctgt
      661 atgagcatgc tgttgctcat tctggattgc ctgacaatgc cagacgatcc ggactcagat
      721 ctggcagcgg tggaggcagc gcatccggcg gaagcggaag cgctcagcag gctgcagaca
      781 agtacctcta tgtggataaa aacttcatca ataacccgct ggcccaagct gactgggctg
      841 ccaagaagtt ggtatgggtg ccttccagca agaatggctt tgaaccagcc agcctcaagg
      901 aggaggtggg agaagaggcc attgtagagc tggtagagaa tgggaagaag gtgaaggtga
      961 acaaggacga catccagaag atgaacccac ccaagttctc caaggtggag gacatggcag
     1021 agctcacgtg cctcaacgaa gcttcggtgc tgcacaacct caaggagcga tactactcag
     1081 ggcttatcta cacctattca ggcctgttct gtgtggtcat caacccttat aagaacctgc
     1141 ccatctactc agaggagatc gtggagatgt acaagggcaa gaagaggcac gagatgccac
     1201 cccacatcta cgccatcaca gatactgcct accggagcat gatgcaggac cgggaagatc
     1261 agtccatcct gtgcacgggg gagtctggag cagggaagac agagaacacc aagaaagtca
     1321 tccagtacct ggcacatgtg gcctcctcac acaagagcaa gaaggaccag ggggagttgg
     1381 agcggcagct gctacaggcc aaccctatcc tagaggcctt tggaaacgcc aagacggtga
     1441 agaatgacaa ctcctctcga ttcggtaaat tcattcgtat caactttgat gtcaatggct
     1501 acattgttgg tgccaacatt gagacttatc ttctggagaa atctcgtgct atccgccaag
     1561 ccaaagagga gcggaccttc cacatcttct actacctgct gtctggggcc ggagaacacc
     1621 tgaagactga tctcctgttg gagccataca acaaataccg cttcctgtcc aacgggcacg
     1681 tcaccatccc tgggcagcag gacaaggaca tgttccagga gacaatggag gccatgagaa
     1741 ttatgggtat cccagaggat gagcagatgg gcttgctgcg ggtcatctct ggggtccttc
     1801 agcttggcaa cattgccttc aagaaggagc ggaacactga ccaggcgtcc atgccggaca
     1861 acacagctgc tcaaaaggtg tcccacctcc tggggatcaa tgtgaccgac ttcaccagag
     1921 gcatcctcac cccacgcatc aaggtgggca gagactatgt gcagaaggcg cagactaaag
     1981 agcaggctga ctttgccatt gaggccttgg ccaaggctac ctatgagcgg atgttccgct
     2041 ggctggtgct tcgcatcaac aaagctctgg acaagaccaa gaggcagggc gcctcattta
     2101 tcgggatact ggacatcgct ggctttgaaa tctttgatct gaactccttc gagcagctgt
     2161 gcatcaacta caccaacgag aagctgcagc agctgttcaa ccacaccatg ttcatcctgg
     2221 agcaggagga gtaccagcga gagggcatcg agtggaactt catcgacttc ggcctggacc
     2281 tgcagccctg catcgacctc attgagaagc cggcgggtcc cccaggcatc ctggccctgc
     2341 tagatgagga gtgctggttt cctaaggcca ctgacaagag cttcgtggag aaggtggtgc
     2401 aggagcaggg cacccacccc aagttccaga agcccaagca actgaaggac aaggctgatt
     2461 tctgcattat ccactatgcc ggcaaggtgg actataaagc tgacgagtgg ctgatgaaga
     2521 acatggaccc cttgaacgac aacatcgcca cgctgcttca ccagtcctca gacaagtttg
     2581 tctctgagct gtggaaggat gtggatcgga tcattggctt ggaccaagtg gctggaatgt
     2641 ccgagacagc actacctggt gccttcaaga cccggaaggg catgttccgt actgtcggac
     2701 agctgtacaa ggagcagctg gccaagctca tggccacgtt gaggaatacc aaccccaact
     2761 tcgtgcgctg catcattccc aaccatgaga agaaggccgg caaactggac ccgcacttgg
     2821 tgctggacca gctgcgctgc aatggcgtcc ttgagggcat ccggatctgc cgccagggct
     2881 ttcccaacag ggtggtcttc caggagttcc ggcagaggta tgagatcctc acccccaact
     2941 ccatcccgaa gggcttcatg gatggcaagc aagcgtgtgt gctcatgatc aaagccttgg
     3001 agcttgacag caacctgtac cgcatcggcc agagcaaagt gttcttccgg gcaggagtgc
     3061 tcgcccacct ggaggaagag cgggacctga agatcaccga tgtcatcatt ggcttccagg
     3121 cctgctgcag gggctacctg gccaggaagg cctttgccaa gaggcagcaa cagctgaccg
     3181 ccatgaaggt cctacagagg aactgtgctg cgtacctcag gctgcgcaac tggcagtggt
     3241 ggaggctctt caccaaggtc aagcccctgt tgaactcaat aagacatgag gatgagctgt
     3301 tagccaagga ggcggaactg acaaaggttc gagagaaaca tctggctgca gagaacaggc
     3361 tgacagagat ggagacgatg cagtctcagc tcatggcaga gaagctgcag cttcaggagc
     3421 agctgcaggc ggagacagag ctgtgtgccg aggctgagga gctccgggcc cgtctgacag
     3481 cgaagaagca ggagctggag gagatatgcc atgacctgga ggccagggtg gaggaggagg
     3541 aggagcgctg ccagtacctg caggccgaga agaagaagat gcagcagaac atccaggaac
     3601 ttgaggagca gttggaggag gaggagagcg cccggcagaa gctgcagctt gagaaggtga
     3661 ccaccgaggc caagctgaag aaactggagg aggaccagat catcatggag gaccagaact
     3721 gcaaactggc caaggagaag aaactgctgg aagacagagt agctgagttc actaccaacc
     3781 tcatggaaga ggaggagaag tccaagagcc tggccaagct caagaacaag cacgaggcaa
     3841 tgatcaccga cctggaagag cgcctccgta gggaggagaa gcagaggcag gagttggaga
     3901 agacccgtcg caagctggag ggagactcca cagacctcag tgaccagatt gctgagctcc
     3961 aggcgcagat agcagagctc aagatgcagc tggccaagaa ggaggaggag ttgcaggctg
     4021 ccttggccag agtggaagaa gaagctgctc agaagaatat ggccctgaag aagatccgag
     4081 aactggaaac tcagatatct gagctccagg aggacctgga gtcggagcga gcctccagga
     4141 ataaagccga gaagcagaaa cgggatctgg gagaggagct ggaggcgctg aagacagagc
     4201 tggaggacac gctggactcc acggctgccc agcaggagct gaggtcgaag cgtgagcagg
     4261 aggtgagcat cctgaagaag actctggagg acgaggccaa gacccatgag gcccagatcc
     4321 aggagatgag gcagaagcac tcacaggctg tggaggagct ggcagatcag ttggagcaga
     4381 cgaagcgggt aaaagctacc cttgagaagg cgaagcagac cctggagaat gagcggggag
     4441 agctggccaa tgaggtgaag gccctgctgc aaggcaaggg cgactcagag cacaagcgca
     4501 agaaggtgga ggcgcagctg caagaactgc aggtcaagtt cagcgaggga gagcgcgtgc
     4561 gaaccgaact ggccgacaag gtcaccaagc tgcaggttga actggacagt gtgaccggtc
     4621 tccttagcca gtctgacagc aagtccagca agcttacgaa ggacttctct gcgctggagt
     4681 cccagcttca ggacacacag gagttgctcc aggaggagaa ccggcagaag ctgagcctga
     4741 gcaccaagct caagcagatg gaggatgaga aaaactcctt cagggagcag ctggaggagg
     4801 aggaggaggc caagcgcaac ttggagaagc agatcgccac gctccatgcc caggtgaccg
     4861 acatgaagaa gaagatggag gacggtgtag ggtgcctgga gactgcagag gaggcgaagc
     4921 ggaggcttca gaaggacttg gaaggcctga gccagcggct tgaggagaag gtggctgcct
     4981 acgataagct ggagaagacc aagacacggc tgcagcagga gctggacgac ctgctggttg
     5041 acctggacca ccagcggcag agcgtctcca acctggaaaa gaagcagaag aagttcgacc
     5101 agctcctagc cgaggagaag accatctcgg ccaagtatgc agaggagcgt gaccgagctg
     5161 aggctgaggc ccgtgagaag gagacaaagg cgctatcact ggcccgggcg cttgaggagg
     5221 ccatggagca gaaggcagag ctggagcggc tcaacaagca gttccgcacg gagatggagg
     5281 acctcatgag ctccaaggat gacgtgggca agagtgtcca cgagctggag aagtccaagc
     5341 gggccttgga gcagcaggtg gaggagatga agacccagct ggaggagctg gaggatgagc
     5401 tgcaggccac ggaggatgcc aagctccgcc tggaggtgaa cctgcaggcc atgaaggccc
     5461 agtttgagcg ggatctgcag ggccgggatg aacagagcga ggagaagaag aagcagctgg
     5521 tcagacaggt gcgggagatg gaggcggagc tggaggatga gaggaagcag cgctccatgg
     5581 ccatggccgc acgcaagaaa ctggagatgg atctgaagga cctggaggca cacattgaca
     5641 cagccaataa gaaccgggaa gaggccatca aacagctgcg gaagcttcag gcccagatga
     5701 aggactgcat gcgggagctg gacgacacgc gcgcctcccg ggaggagatc ctggcgcagg
     5761 ccaaggagaa tgagaagaag ctgaagagca tggaggccga gatgattcag ctgcaggagg
     5821 aactggcagc tgctgagcgt gctaagcgtc aggcccaaca ggaacgggac gagctggctg
     5881 atgagatcgc caacagcagt ggcaaagggg ccctagcatt agaggagaag cggcgactgg
     5941 aggcccgcat tgccctgctg gaggaggagc tggaggagga acagggcaac acggagctga
     6001 tcaacgatcg gctgaagaag gccaacctgc agatcgacca aataaacacc gacctgaacc
     6061 tggaacgcag ccacgcacag aagaatgaga atgcgcgaca gcagctggaa cgccagaaca
     6121 aggagctcaa ggccaagctg caggaaatgg agagtgctgt caagtccaaa tacaaggcct
     6181 ccatcgcggc cttggaggcc aaaattgcac agctggagga acagctggac aacgagacca
     6241 aggagcgcca ggcagcctcc aagcaggtgc gccggacgga gaagaagctg aaggacgtgc
     6301 tgctgcaggt ggaggacgag cggaggaacg cggaacagtt caaggaccag gctgacaagg
     6361 cgtccacccg cctgaagcag cttaaacggc agctagagga ggctgaagag gaggcccagc
     6421 gggccaatgc ctcacgccgg aagctgcagc gtgagctgga agatgccaca gagaccgctg
     6481 atgctatgaa ccgcgaggtc agctccctga agaacaaact gaggcgtggg gacctgccat
     6541 ttgtcgtgac tcgccgaatt gttcggaaag gcactggcga ctgctcagac gaggaggtcg
     6601 acggtaaagc agatggggcc gatgccaagg cagctgaata ggaattctgc agtcgacggt
     6661 accgcgggcc cgggatccac cggatctaga taactgat
//
