LOCUS       sequence-88609-e        6797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 507 / 572, Emission: 516 / 
            580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6797)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos3.2-MyosinIIC-N-18
FEATURES             Location/Qualifiers
     source          1..6797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6081..6761
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 acgtcagatc cgctagcacc gccaccatgg ctgcagtgac catgtccgtg tctgggagga
       61 aggtagcctc caggccaggc ccggtgcctg aggcagccca atcgttcctc tacgcgcccc
      121 ggacgccaaa tgtaggtggc cctggagggc cacaggtgga gtggacagcc cggcgcatgg
      181 tgtgggtgcc ctcggaactg catgggttcg aggcagcagc cctgcgggat gaaggggagg
      241 aggaggcaga agtggagctg gcggagagtg ggcgccgcct gcggctgccc agggaccaga
      301 tccagcgcat gaacccaccc aagttcagca aggcagaaga tatggctgag ctcacctgcc
      361 tcaacgaggc ctcggtcctg cacaacctgc gagaacgcta ctactccggg ctcatttata
      421 cctactctgg cctcttctgt gtggtcatta acccatacaa gcagctgccc atctacacgg
      481 aggccattgt tgaaatgtac cggggcaaga agcgccatga ggtgccacct cacgtgtatg
      541 ctgtgacgga gggcgcgtac cgcagcatgc ttcaggatcg tgaggatcaa tccattctct
      601 gcacgggaga gtctggcgct gggaagacgg agaacaccaa gaaggtcatc cagtacctgg
      661 cccatgtggc atcatctcca aagggcagga aggagcctgg tgtccctgcc tccgtcagca
      721 ccatgtctta tggggagcta gagcgtcagc ttcttcaagc caaccccatc ctagaggcct
      781 ttggcaatgc caagacagtg aagaacgaca actcttcccg atttggcaaa ttcatccgca
      841 tcaactttga tattgctggc tacatcgtgg gagcaaacat cgagacctat ctgttggaga
      901 agtcccgggc catcagacag gccaaggatg aatgcagctt ccatatcttc taccagctgc
      961 tagggggcgc tggggagcag ctaaaagctg acctccttct ggagccctgt tcccattatc
     1021 gcttcctgac caatgggccc tcatcgtccc cgggccagga gcgtgagtta ttccaggaga
     1081 ccctggagtc cctgcgtgtg ctgggcctcc tcccagaaga gatcactgcc atgctgcgca
     1141 ctgtctctgc tgtcctccag tttggcaaca ttgtcctgaa gaaagagcgc aatacggacc
     1201 aagccaccat gcctgacaac acagctgccc agaagctttg ccgcctcttg ggactcggag
     1261 tgaccgactt ctccagagcc cttctcacac cccgcatcaa agtgggccga gattatgttc
     1321 agaaagcaca aaccaaggag caggctgact ttgcgctgga ggctctggcc aaagctacct
     1381 atgagcgcct gttccgctgg ctggttctgc ggctcaaccg tgccctggac agaagcccgc
     1441 ggcagggtgc ctccttcctg ggcatcctgg acatcgcggg ctttgagatc ttccagctga
     1501 actccttcga gcagctgtgc atcaactaca ccaacgagaa gctacagcag ctattcaacc
     1561 acaccatgtt cgtgctggag caggaggagt accagcgaga gggcatcccc tggaccttcc
     1621 tagacttcgg gttggacctg caaccttgca tcgacctcat tgagcgtccg gccaaccctc
     1681 caggtctcct ggccctgctg gacgaggagt gctggttccc caaggccacg gacaagtctt
     1741 ttgtggagaa ggtcgcccag gagcagggca gccaccccaa attccagcgc cccaggaacc
     1801 tgcgagatca ggccgacttc agcgtcctgc actacgccgg caaggttgac tacaaagcca
     1861 gtgagtggct gatgaagaac atggacccac tgaatgacaa tgtggccgcc ttgcttcacc
     1921 agagcacgga tcgtctcaca gctgagatct ggaaggatgt ggagggcatc gtggggctgg
     1981 agcaagtaag cagccttgga gatggcccac cgggaggccg cccccgccgt ggaatgtttc
     2041 ggactgtggg gcagctctac aaagaatccc tgagccgcct catggccacg ctcagcaaca
     2101 ccaaccctag ttttgtccgc tgcatcgttc ccaatcatga gaagagggct ggaaagctgg
     2161 agccgcgcct ggtgctggac caactccgtt gtaacggggt cctcgagggt atacgcatct
     2221 gtcgccaagg cttccccaac cgcatcctct tccaggagtt ccgacagcgc tatgaaatcc
     2281 tcaccccgaa cgctattccc aagggcttca tggacggcaa acaggcctgt gagaagatga
     2341 tccaggccct ggagctagac cccaacctgt accgtgttgg ccaaagcaag atcttcttcc
     2401 gggcaggggt cctggcccag ctggaggagg agcgggacct gaaagtcacc gacatcatag
     2461 tgtctttcca ggcagcggca cggggctacc tggcccgtag ggctttccag agacggcagc
     2521 agcagcagag tgctctgagg gtgatgcaga gaaactgtgc tgcctacctc aagctcagga
     2581 actggcagtg gtggaggctg ttcatcaagg tgaagcccct gctgcaggtg acacggcagg
     2641 atgaggtgct gcaggcgcgc gcccaggagc tgcagaaagt tcaggagctg cagcagcaga
     2701 gcgctcgtga agtgggggaa ctgcagggtc gagtggcaca gctagaggag gagcgcacgc
     2761 gcctggctga gcagcttcga gcagaagccg agctctgctc tgaggccgag gagacgcggg
     2821 cgcgactggc tgcccggaag caggagctgg agctggtggt gacagagctg gaggcacgag
     2881 tgggcgagga agaagagtgc agccggcagc tgcagagtga gaagaagagg ctgcagcagc
     2941 atatccagga gctagagagc cacctggaag ctgaggaggg tgcccggcag aagctacagc
     3001 tggagaaggt gaccacagag gccaagatga agaaatttga ggaggacctg ctgctcctgg
     3061 aggaccagaa ttccaagctg agcaaggagc ggaggctgct ggaggagcgg ctggctgagt
     3121 tctcctcaca ggcagcagaa gaggaagaga aagtcaaaag tctcaacaag ctgaggctca
     3181 aatatgaagc cacaatctca gacatggaag accggctgaa gaaggaggag aagggacgcc
     3241 aggaactaga gaagctgaag cgacggctgg acggggagag ctcagagctt caggagcaga
     3301 tggtggagca gaagcagagg gcagaggaac tgctcgcaca gctgggccgc aaggaggatg
     3361 agctgcaggc cgccctgctc agggcagagg aagagggtgg tgcccgtgcc cagttgctca
     3421 agtccctgcg agaggcacag gctggccttg ctgaggctca ggaggacctg gaagctgagc
     3481 gggtagccag ggccaaggcg gagaagcagc gccgggacct gggcgaggag ttggaggccc
     3541 tacgtgggga gctcgaggac actctggatt ccaccaacgc ccagcaggag ctgcggtcca
     3601 agagggagca ggaggtgaca gagctgaaga aagcattgga agaggagtcc cgtgcccatg
     3661 aggtgtccat gcaggagctg agacagaggc atagccaggc actggtggag atggccgagc
     3721 agttggagca agcccggagg ggcaaaggtg tgtgggagaa gactcggcta tccctggagg
     3781 ctgaggtgtc cgagctgaag gccgagctga gcagcctgca gacctcgaga caggagggtg
     3841 agcagaagag gcgccgcctg gagtcccagc tacaggaggt ccagggccga tccagtgatt
     3901 cggagcgggc tcggtctgag gctgctgaga agctgcagag agcccaggcg gaacttgaga
     3961 gcgtgtccac agccctgagt gaggcggagt ccaaagccat caggctgggc aaggagctga
     4021 gcagtgcaga gtcccagctg catgacaccc aggaactgct tcaggaggag accagggcaa
     4081 agctggcctt ggggtcccgt gtgcgtgccc tagaggccga ggcggcgggg cttcgggagc
     4141 agatggaaga ggaggtggtt gccagggaac gggctggccg ggagctgcag agcacgcagg
     4201 cccagctctc tgaatggcgg cgccgccagg aagaagaggc tgcggtgctg gaggctgggg
     4261 aggaggctcg gcgccgtgca gcccgggagg cagagaccct gacccagcgc ctggcagaaa
     4321 agactgaggc tgtagaacga ctggagcgag cccggcgccg actgcagcag gagttggacg
     4381 atgccactgt ggatctgggg cagcagaagc agctcctgag cacactggag aagaagcagc
     4441 ggaaatttga ccagctcctg gcagaggaga aggctgcagt tctacgggct gtggaagacc
     4501 gtgaacggat agaggccgaa ggccgggagc gagaggcccg ggccctgtcg ctgacccggg
     4561 ccctggaaga ggagcaggag gcccgggagg agctggagag gcagaaccgt gctctgaggg
     4621 ctgagctgga agcactgctg agcagcaagg atgacgtggg caagaacgtg cacgagctgg
     4681 agcgagcccg taaggcggct gaacaggcag ccagtgacct gcggacacag gtgacagaat
     4741 tggaggatga gctgacagcc gcagaggatg ccaagctgcg cctggaggtg actgtgcagg
     4801 ctctgaaggc tcaacatgaa cgcgacctgc agggccgcga tgatgccggt gaggagaggc
     4861 ggaggcagct ggccaagcag ctaagagacg cagaggtaga gcgcgatgag gaacggaagc
     4921 agagggcact ggctatggct gcccgcaaga agctggagct ggaactggag gagttgaagg
     4981 cgcagacatc tgctgctggg cagggcaagg aagaggcagt gaagcagctg aagaagatgc
     5041 aggtccagat gaaggagctg tggcgggagg tagaggagac gcgtagctcc cgcgacgaga
     5101 tgtttaccct gagcagggaa aatgagaaga agctcaaggg gctggaagct gaggtgctgc
     5161 gtctgcaaga ggaacttgct gcctcagacc gagcccggag gcaggcccag caagacagag
     5221 acgagatggc agaggaggtg gccagtggca atcttagcaa ggcagccacc ctggaggaaa
     5281 aacggcagct ggaggggcga ctgagccagt tggaagagga gctggaggaa gaacagaaca
     5341 actcggagct gctcaaggac cattaccgaa agctagtgct acaggtcgag tccctcacca
     5401 cagaactgtc tgccgaacga agtttctcag ccaaggccga gagtggacgg cagcagctgg
     5461 agcggcagat ccaggaactg cgggcccgct tgggtgaaga ggatgctgga gcccgagcca
     5521 ggcagaaaat gctgatcgct gctctggagt ctaaactggc ccaggcagag gagcagctgg
     5581 agcaggagag cagggagcgc atcctctctg gcaagctggt acgcagagct gagaagcggc
     5641 tgaaggaggt agttcttcag gtggatgaag agcgcagggt ggctgaccag gtccgggacc
     5701 agctggagaa aagcaacctc cggctgaagc agctcaagag gcagctggag gaggcagagg
     5761 aggaggcatc tcgggcacag gctggtcgga ggcggctgca gcgggagctg gaggacgtca
     5821 ctgagtctgc agaatccatg aaccgggagg tgaccacgct gaggaacagg ctccggcgtg
     5881 gcccacttac attcaccaca cggactgtgc gccaggtgtt ccggctggaa gagggcgtgg
     5941 cttctgacga ggaagaggct gaaggagctg aacctggctc tgcaccaggc caggagccgg
     6001 aggctccgcc ccctgccaca ccccaaagca gcggtggagc aagcgcagcc agtggtagcg
     6061 cggatccacc ggtcgccacc atgagtgcga ttaagccaga catgaagatc aaactccgta
     6121 tggaaggcaa cgtaaacggg caccactttg tgatcgacgg agatggtaca ggcaagcctt
     6181 ttgagggaaa acagagtatg gatcttgaag tcaaagaggg cggacctctg ccttttgcct
     6241 ttgatatcct gaccactgca ttccattacg gcaacagggt attcgccaaa tatccagaca
     6301 acatacaaga ctattttaag cagtcgtttc ctaaggggta ttcgtgggaa cgaagcttga
     6361 ctttcgaaga cgggggcatt tgcaacgcca gaaacgacat aacaatggaa ggggacactt
     6421 tctataataa agttcgattt tatggtacca actttcccgc caatggtcca gttatgcaga
     6481 agaagacgct gaaatgggag ccctccactg agaaaatgta tgtgcgtgat ggagtgctga
     6541 cgggtgatat tgagatggct ttgttgcttg aaggaaatgc ccattaccga tgtgacttca
     6601 gaactactta caaagctaag gagaagggtg tcaagttacc aggcgcccac tttgtggacc
     6661 actgcattga gattttaagc catgacaaag attacaacaa ggttaagctg tatgagcatg
     6721 ctgttgctca ttctggattg cctgacaatg ccagacgata agcggccgcg actctagatc
     6781 ataatcagcc ataccac
//
