LOCUS       sequence-88611-e        1379 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endosomes, Excitation: 507 / 572, Emission: 516 / 580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1379)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos3.2-NEEP21-N-18
FEATURES             Location/Qualifiers
     source          1..1379
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(120..131)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(141..152)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             645..1325
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 acatttctag tgacgtcaga tccgctagcg ccaccatggt gaagttgggg aataatttcg
       61 cagagaaggg caccaagcag ccactgctgg aggatggctt cgacaccatt cctttgatga
      121 cgcccctcga tgtcaaccag ctgcagttcc cacccccaga taaggtcgtg gtgaaaacta
      181 agactgaata tgaacctgat cgcaaaaaag gaaaagcacg tcctcccaag atagccgagt
      241 tcaccgtcag catcaccgag ggtgtcaccg agaggtttaa ggtctccgtg ctggtcctct
      301 ttgccctggc cttcctcacc tgtgtcgtct tcctggttgt ctacaaagtg tacaagtatg
      361 accgcgcctg ccctggtggg tttgtcttga agaacaccca gtgcatccca gaaggcttgg
      421 agagctacta cacggagcaa gactccagtg cccgggagaa attttacact gtcataaacc
      481 actacaacgt ggccaagcag agcatcaccc gctccgtgtc gccatggatg tcagttctgt
      541 cagaagagaa gctgtcggaa caggagaccg aagctgcaga gaagtcagcc agcagcggtg
      601 gagcaagcgc agccagtggt agcgcggatc caccggtcgc caccatgagt gcgattaagc
      661 cagacatgaa gatcaaactc cgtatggaag gcaacgtaaa cgggcaccac tttgtgatcg
      721 acggagatgg tacaggcaag ccttttgagg gaaaacagag tatggatctt gaagtcaaag
      781 agggcggacc tctgcctttt gcctttgata tcctgaccac tgcattccat tacggcaaca
      841 gggtattcgc caaatatcca gacaacatac aagactattt taagcagtcg tttcctaagg
      901 ggtattcgtg ggaacgaagc ttgactttcg aagacggggg catttgcaac gccagaaacg
      961 acataacaat ggaaggggac actttctata ataaagttcg attttatggt accaactttc
     1021 ccgccaatgg tccagttatg cagaagaaga cgctgaaatg ggagccctcc actgagaaaa
     1081 tgtatgtgcg tgatggagtg ctgacgggtg atattgagat ggctttgttg cttgaaggaa
     1141 atgcccatta ccgatgtgac ttcagaacta cttacaaagc taaggagaag ggtgtcaagt
     1201 taccaggcgc ccactttgtg gaccactgca ttgagatttt aagccatgac aaagattaca
     1261 acaaggttaa gctgtatgag catgctgttg ctcattctgg attgcctgac aatgccagac
     1321 gataagcggc cgcgactcta gatcataatc agccatacca cattgtagaa gatttccgc
//
