LOCUS       sequence-88612-e        2158 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Pore, Excitation: 507 / 572, Emission: 516 / 
            580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2158)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos3.2-Nup50-N-10
FEATURES             Location/Qualifiers
     source          1..2158
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1459..2139
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gtcagatccg ctagcgatgc caccatggcc aaaagaaatg ccgagaagga actgacagat
       61 aggaattggg atcaagaaga tgaagctgaa gaggtgggaa cattctccat ggccagtgag
      121 gaagtcttga agaatagagc cataaagaaa gcaaagcgca gaaatgttgg atttgaatct
      181 gacactggag gagcctttaa aggttttaaa ggtttggtgg taccttctgg aggaggacgc
      241 ttttctggat ttggtagtgg cgctggaggg aagcctctgg aaggactgtc gaatggaaac
      301 aacataacca gtgcccctcc cttcgccagt gcaaaggcag cggcagatcc caaggtagcc
      361 tttggttctc ttgctgcaaa tggccctacc accttggttg ataaagtttc aaatcccaaa
      421 actaatgggg acagtcagca gccctcctcc tctggccttg cttccagtaa agcttgtgtc
      481 ggaaatgcct atcacaagca gttggccgcc ttgaactgct ccgtgcggga ttggatagtg
      541 aagcacgtga atacaaaccc cctctgtgat ctgacaccta tctttaaaga ctatgagaaa
      601 tatttagcaa acattgaaca gcaacacggg aacagtggca ggaattctga aagtgaatct
      661 aacaaagtgg cagctgaaac acagtctcct tccctttttg gctcaacaaa attacagcaa
      721 gagtcaacgt ttttgtttca tggcaacaaa actgaagata cacctgacaa gaagatggag
      781 gtggcatctg aaaagaaaac ggacccatca tcactaggag cgacaagtgc ctcatttaat
      841 ttcggcaaga aagttgatag ctctgttttg ggctcattaa gctctgtccc cctgactgga
      901 ttttctttct cccctggaaa ctccagttta tttggcaaag atactaccca gagtaaacca
      961 gtctcttcac catttcccac taaaccattg gagggccaag cagaaggtga cagtggtgaa
     1021 tgcaaaggtg gagatgaaga agagaatgat gagccaccca aagtagtagt taccgaagta
     1081 aaagaagaag acgcttttta ctccaaaaag tgtaaactgt tttacaagaa agacaatgag
     1141 tttaaagaga aaggcatagg tactctgcat ttaaaaccta cagcaaatca gaagacacag
     1201 cttttggtgc gggcagacac caatttaggc aacatattgc tgaacgttct gattccaccc
     1261 aatatgccat gtacgcgaac agggaagaat aacgttctta tcgtctgtgt tccaaatcca
     1321 ccaattgacg agaagaatgc caccatgcca gtcaccatgt tgattcgggt aaaaaccagc
     1381 gaggatgcag acgagttgca caaaatttta ctggagaaaa aggatgccag ctccggaggg
     1441 gatccaccgg tcgccaccat gagtgcgatt aagccagaca tgaagatcaa actccgtatg
     1501 gaaggcaacg taaacgggca ccactttgtg atcgacggag atggtacagg caagcctttt
     1561 gagggaaaac agagtatgga tcttgaagtc aaagagggcg gacctctgcc ttttgccttt
     1621 gatatcctga ccactgcatt ccattacggc aacagggtat tcgccaaata tccagacaac
     1681 atacaagact attttaagca gtcgtttcct aaggggtatt cgtgggaacg aagcttgact
     1741 ttcgaagacg ggggcatttg caacgccaga aacgacataa caatggaagg ggacactttc
     1801 tataataaag ttcgatttta tggtaccaac tttcccgcca atggtccagt tatgcagaag
     1861 aagacgctga aatgggagcc ctccactgag aaaatgtatg tgcgtgatgg agtgctgacg
     1921 ggtgatattg agatggcttt gttgcttgaa ggaaatgccc attaccgatg tgacttcaga
     1981 actacttaca aagctaagga gaagggtgtc aagttaccag gcgcccactt tgtggaccac
     2041 tgcattgaga ttttaagcca tgacaaagat tacaacaagg ttaagctgta tgagcatgct
     2101 gttgctcatt ctggattgcc tgacaatgcc agacgataag cggccgcgac tctagatc
//
