LOCUS       sequence-88613-e        2972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Scaffold, Excitation: 507 / 572, Emission: 
            516 / 580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2972)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos3.2-PSD95-N-14
FEATURES             Location/Qualifiers
     source          1..2972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2256..2936
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 cagatccgct agcgctaccg gactcagatc tcgaggccac catggactgt ctctgtatag
       61 tgacaaccaa gaaataccgc taccaagatg aagacacgcc ccctctggaa cacagcccgg
      121 cccacctccc caaccaggcc aattctcccc ctgtgattgt caacacggac accctagaag
      181 ccccaggata tgagttgcag gtgaatggaa cagaggggga gatggagtat gaggagatca
      241 cattggaaag gggtaactca ggtctgggct tcagcatcgc aggtggcact gacaacccgc
      301 acatcggtga cgacccgtcc atttttatca ccaagatcat tcctggtggg gctgcagccc
      361 aggatggccg cctcagggtc aatgacagca tcctgtttgt aaatgaagtg gatgttcggg
      421 aggtgaccca ttcagctgcg gtggaggccc tcaaagaggc aggttccatc gttcgcctct
      481 atgtcatgcg ccggaaaccc ccagccgaaa aggtcatgga gatcaaactc atcaaagggc
      541 ctaaaggact tggcttcagc attgcggggg gcgttgggaa ccagcacatc cctggagata
      601 acagcatcta tgtaacgaag atcatcgaag gaggtgctgc ccacaaggat ggcaggttgc
      661 agattggaga caagatcctg gcggtcaaca gtgtggggct ggaggacgtc atgcacgagg
      721 atgccgtggc agccctgaag aacacatatg acgttgtgta cctaaaggtg gccaagccca
      781 gcaatgccta cctgagtgac agctatgctc ccccagacat cacaacctcg tattctcagc
      841 acctggacaa tgagatcagt catagcagct acttgggcac tgactacccc acagccatga
      901 cccccacttc ccctcggcgc tactcccctg tggccaagga cctgctgggg gaggaagaca
      961 ttccccggga accaaggcgg atcgtgatcc atcggggctc caccggcctg ggcttcaaca
     1021 tcgtgggcgg cgaggatggt gaaggcatct tcatctcctt catccttgct gggggtccag
     1081 ccgacctcag tggggagcta cggaaggggg accagatcct gtcggtcaat ggtgttgacc
     1141 tccgcaatgc cagtcacgaa caggctgcca ttgccctgaa gaatgcgggt cagacggtca
     1201 cgatcatcgc tcagtataaa ccagaagagt atagtcgatt cgaggccaag atccatgatc
     1261 ttcgggaaca gctcatgaat agtagcctag gctcagggac tgcatccttg cgaagcaacc
     1321 ccaagagggg cttctacatt agggccctgt ttgattacga caagaccaag gactgcggtt
     1381 tcttgagcca ggccctgagc ttccgcttcg gggatgtgct tcatgtcatt gacgctggtg
     1441 acgaagagtg gtggcaagca cggcgggtcc actccgacag tgagaccgac gacattggct
     1501 tcattcccag caaacggcgg gtcgagcgac gagagtggtc aaggttaaag gccaaggact
     1561 ggggctccag ctctggatca cagggtcgag aagactcggt tctgagctat gagacggtga
     1621 cccagatgga agtgcactat gctcgtccca tcatcatcct tggacccacc aaagaccgtg
     1681 ccaacgatga tcttctctcc gagttccccg acaagtttgg atcctgtgtc cctcatacga
     1741 cacgtcctaa gcgggaatat gagatagacg gccgggatta ccactttgtc tcctcccggg
     1801 agaaaatgga gaaggacatc caggcacaca agttcattga ggctggccag tacaacagcc
     1861 acctctatgg gaccagcgtc cagtctgtgc gagaggtagc agagcagggg aagcactgca
     1921 tcctcgatgt ctcggccaat gccgtgcggc ggctgcaggc ggcccacctg caccccatcg
     1981 ccatcttcat ccgtccccgc tccctggaga atgtgctaga gatcaataag cggatcacag
     2041 aggagcaagc ccggaaagcc ttcgacagag ccacgaagct ggagcaggag ttcacagagt
     2101 gcttctcagc catcgtagag ggcgacagct ttgaagagat ctatcacaaa gtgaaacgtg
     2161 tcattgaaga cctctcaggc ccctacatct gggtcccagc ccgagagaga ctcggcagcg
     2221 catccggcgg aagcggaagc ggaccggtcg ccaccatgag tgcgattaag ccagacatga
     2281 agatcaaact ccgtatggaa ggcaacgtaa acgggcacca ctttgtgatc gacggagatg
     2341 gtacaggcaa gccttttgag ggaaaacaga gtatggatct tgaagtcaaa gagggcggac
     2401 ctctgccttt tgcctttgat atcctgacca ctgcattcca ttacggcaac agggtattcg
     2461 ccaaatatcc agacaacata caagactatt ttaagcagtc gtttcctaag gggtattcgt
     2521 gggaacgaag cttgactttc gaagacgggg gcatttgcaa cgccagaaac gacataacaa
     2581 tggaagggga cactttctat aataaagttc gattttatgg taccaacttt cccgccaatg
     2641 gtccagttat gcagaagaag acgctgaaat gggagccctc cactgagaaa atgtatgtgc
     2701 gtgatggagt gctgacgggt gatattgaga tggctttgtt gcttgaagga aatgcccatt
     2761 accgatgtga cttcagaact acttacaaag ctaaggagaa gggtgtcaag ttaccaggcg
     2821 cccactttgt ggaccactgc attgagattt taagccatga caaagattac aacaaggtta
     2881 agctgtatga gcatgctgtt gctcattctg gattgcctga caatgccaga cgataagcgg
     2941 ccgcgactct agatcataat cagccatacc ac
//
