LOCUS       sequence-88614-e        2728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centromere, Excitation: 507 / 572, Emission: 516 / 
            580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2728)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos3.2-Rad21-C-18
FEATURES             Location/Qualifiers
     source          1..2728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..722
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gcatgctagt gacgtcagat ccgctagcgc taccggtcgc caccatgagt gcgattaagc
       61 cagacatgaa gatcaaactc cgtatggaag gcaacgtaaa cgggcaccac tttgtgatcg
      121 acggagatgg tacaggcaag ccttttgagg gaaaacagag tatggatctt gaagtcaaag
      181 agggcggacc tctgcctttt gcctttgata tcctgaccac tgcattccat tacggcaaca
      241 gggtattcgc caaatatcca gacaacatac aagactattt taagcagtcg tttcctaagg
      301 ggtattcgtg ggaacgaagc ttgactttcg aagacggggg catttgcaac gccagaaacg
      361 acataacaat ggaaggggac actttctata ataaagttcg attttatggt accaactttc
      421 ccgccaatgg tccagttatg cagaagaaga cgctgaaatg ggagccctcc actgagaaaa
      481 tgtatgtgcg tgatggagtg ctgacgggtg atattgagat ggctttgttg cttgaaggaa
      541 atgcccatta ccgatgtgac ttcagaacta cttacaaagc taaggagaag ggtgtcaagt
      601 taccaggcgc ccactttgtg gaccactgca ttgagatttt aagccatgac aaagattaca
      661 acaaggttaa gctgtatgag catgctgttg ctcattctgg attgcctgac aatgccagac
      721 gatccggact cagatctcga ggaagcggcg gatctgggtc cggcagctcc ggcagcttct
      781 acgcacattt tgttctcagt aaaagagggc ctctggccaa aatttggcta gcggcccatt
      841 gggataagaa gctaaccaaa gcccatgtgt tcgagtgtaa tttagagagc agcgtggaga
      901 gtatcatctc accaaaggtg aaaatggcat tacggacatc aggacatctc ttactgggag
      961 tagttcgaat ctatcacagg aaagccaaat accttcttgc agactgtaat gaagcattca
     1021 ttaagataaa gatggctttt cggccaggtg tggttgacct gcctgaggaa aatcgggaag
     1081 cagcttataa tgccattact ttacctgaag aatttcatga ctttgatcag ccactgcctg
     1141 acttagatga catcgatgtg gcccagcagt tcagcttgaa tcagagtaga gtggaagaga
     1201 taaccatgag agaagaagtt gggaacatca gtattttaca agaaaatgat tttggtgatt
     1261 ttggaatgga tgatcgtgag ataatgagag aaggcagtgc ttttgaggat gacgacatgt
     1321 tagtaagcac tactacttct aacctcctat tagagtctga acagagcacc agcaatctga
     1381 atgagaaaat taaccattta gaatatgaag atcaatataa ggatgataat tttggagaag
     1441 gaaatgatgg tggaatatta gatgacaaac ttattagtaa taatgatggc ggtatctttg
     1501 atgatccccc tgccctctct gaggcagggg tgatgttgcc agagcagcct gcacatgacg
     1561 atatggatga ggatgataat gtatcaatgg gtgggcctga tagtcctgat tcagtggatc
     1621 ccgttgaacc aatgccaacc atgactgatc aaacaacact tgttccaaat gaggaagaag
     1681 catttgcatt ggagcctatt gatataactg ttaaagaaac aaaagccaag aggaagagga
     1741 agctaattgt tgacagtgtc aaagagttgg atagcaagac aattagagcc caacttagtg
     1801 attattcaga tattgttact actttggatc tggcaccgcc caccaagaaa ttgatgatgt
     1861 ggaaagagac aggaggagta gaaaaactgt tttctttacc tgctcagcct ttgtggaata
     1921 acagactact gaagctcttt acacgctgtc ttacaccgct tgtaccagaa gaccttagaa
     1981 aaaggaggaa aggaggagag gcagataatt tggatgaatt cctcaaagaa tttgaaaatc
     2041 cagaggttcc tagagaggac cagcaacagc agcatcagca gcgtgatgtt atcgatgagc
     2101 ccattattga agagccaagc cgcctccagg agtcagtgat ggaggccagc agaacaaaca
     2161 tagatgagtc agctatgcct ccaccaccac ctcagggagt taagcgaaaa gctggacaaa
     2221 ttgacccaga gcctgtgatg cctcctcagc aggtagagca gatggaaata ccacctgtag
     2281 agcttccccc agaagaacct ccaaatatct gtcagctaat accagagtta gaacttctgc
     2341 cagaaaaaga gaaggagaaa gagaaggaaa aagaagatga tgaagaggaa gaggatgaag
     2401 atgcatcagg gggcgatcaa gatcaggaag aaagaagatg gaacaaaagg actcagcaga
     2461 tgcttcatgg tcttcagcgt gctcttgcta aaactggagc tgaatctatc agtttgcttg
     2521 agttatgtcg aaatacgaac agaaaacaag ctgccgcaaa gttctacagc ttcttggttc
     2581 ttaaaaagca gcaagctatt gagctgacac aggaagaacc gtacagtgac atcatcgcaa
     2641 cacctggacc aaggttccat attatataag gtaccgcggg cccgggatcc accggatcta
     2701 gataactgat cataatcagc cataccac
//
