LOCUS       sequence-88615-e        2046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 507 / 572, Emission: 516 
            / 580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2046)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos3.2-TES-N-14
FEATURES             Location/Qualifiers
     source          1..2046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1324..2004
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 agatccgcta gcgccaccat ggacctggaa aacaaagtga agaagatggg cttaggtcac
       61 gagcaaggat ttggagcccc ttgtttaaaa tgcaaagaaa aatgtgaagg attcgaactg
      121 cacttctgga gaaaaatatg tcgtaactgc aagtgtggcc aagaagagca tgatgtcctc
      181 ttgagcaatg aagaggatcg aaaagtggga aaactttttg aagacaccaa gtataccact
      241 ctgattgcaa aactaaagtc agatggaatt cccatgtata aacgcaatgt tatgatattg
      301 acgaatccag ttgctgccaa gaagaatgtc tccatcaata cagttaccta tgagtgggct
      361 cctcctgtcc agaatcaagc attggccagg cagtacatgc agatgctacc caaggaaaag
      421 cagccagtag caggctcaga gggggcacag taccggaaga agcagctggc aaagcagctc
      481 cctgcacatg accaggaccc ttcaaagtgc catgagttgt ctcccagaga ggtgaaggag
      541 atggagcagt ttgtgaagaa atataagagc gaagctctgg gagtaggaga tgtcaaactt
      601 ccctgtgaga tggatgccca aggccccaaa caaatgaaca ttcctggagg ggatagaagc
      661 accccagcag cagtgggggc catggaggac aaatctgctg agcacaaaag aactcaatat
      721 tcctgctatt gctgcaaact gagtatgaaa gaaggtgacc cagccatcta tgccgaaagg
      781 gctggctatg ataaactgtg gcacccagct tgttttgtct gcagcacctg ccatgaactc
      841 ctggttgaca tgatttattt ttggaagaat gagaagctat actgtggcag acattactgt
      901 gacagcgaga aaccccgatg tgctggctgt gacgagctga tattcagcaa tgagtatacc
      961 caggcagaaa accagaattg gcacctgaaa cacttctgct gctttgactg tgatagcatt
     1021 ctagctgggg agatatacgt gatggtcaat gacaagcccg tgtgcaagcc ctgctatgtg
     1081 aagaatcacg ctgtggtgtg tcaaggatgc cacaatgcca tcgacccaga agtgcagcgg
     1141 gtgacctata acaatttcag ctggcatgca tccacagagt gctttctgtg ctcttgctgc
     1201 agcaaatgcc tcattgggca gaagttcatg ccagtagaag ggatggtttt ctgttcagtg
     1261 gaatgtaaga agaggatgtc ttccggcggt agcggcggta gcggggatcc accggtcgcc
     1321 accatgagtg cgattaagcc agacatgaag atcaaactcc gtatggaagg caacgtaaac
     1381 gggcaccact ttgtgatcga cggagatggt acaggcaagc cttttgaggg aaaacagagt
     1441 atggatcttg aagtcaaaga gggcggacct ctgccttttg cctttgatat cctgaccact
     1501 gcattccatt acggcaacag ggtattcgcc aaatatccag acaacataca agactatttt
     1561 aagcagtcgt ttcctaaggg gtattcgtgg gaacgaagct tgactttcga agacgggggc
     1621 atttgcaacg ccagaaacga cataacaatg gaaggggaca ctttctataa taaagttcga
     1681 ttttatggta ccaactttcc cgccaatggt ccagttatgc agaagaagac gctgaaatgg
     1741 gagccctcca ctgagaaaat gtatgtgcgt gatggagtgc tgacgggtga tattgagatg
     1801 gctttgttgc ttgaaggaaa tgcccattac cgatgtgact tcagaactac ttacaaagct
     1861 aaggagaagg gtgtcaagtt accaggcgcc cactttgtgg accactgcat tgagatttta
     1921 agccatgaca aagattacaa caaggttaag ctgtatgagc atgctgttgc tcattctgga
     1981 ttgcctgaca atgccagacg ataagcggcc gcgactctag atcataatca gccataccac
     2041 attgta
//
