LOCUS       sequence-88616-e        1206 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondrial Membrane, Excitation: 507 / 572, 
            Emission: 516 / 580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1206)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos3.2-TOMM20-N-10
FEATURES             Location/Qualifiers
     source          1..1206
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             488..1168
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 cgtcagatcc gctagcgcca ccatggtggg tcggaacagc gccatcgccg ccggtgtatg
       61 cggggccctt ttcattgggt actgcatcta cttcgaccgc aaaagacgaa gtgaccccaa
      121 cttcaagaac aggcttcgag aacgaagaaa gaaacagaag cttgccaagg agagagctgg
      181 gctttccaag ttacctgacc ttaaagatgc tgaagctgtt cagaagttct tccttgaaga
      241 aatacagctt ggtgaagagt tactagctca aggtgaatat gagaagggcg tagaccatct
      301 gacaaatgca attgctgtgt gtggacagcc acagcagtta ctgcaggtct tacagcaaac
      361 tcttccacca ccagtgttcc agatgcttct gactaagctc ccaacaatta gtcagagaat
      421 tgtaagtgct cagagcttgg ctgaagatga tgtggaaggc ggtagcgggg atccaccggt
      481 cgccaccatg agtgcgatta agccagacat gaagatcaaa ctccgtatgg aaggcaacgt
      541 aaacgggcac cactttgtga tcgacggaga tggtacaggc aagccttttg agggaaaaca
      601 gagtatggat cttgaagtca aagagggcgg acctctgcct tttgcctttg atatcctgac
      661 cactgcattc cattacggca acagggtatt cgccaaatat ccagacaaca tacaagacta
      721 ttttaagcag tcgtttccta aggggtattc gtgggaacga agcttgactt tcgaagacgg
      781 gggcatttgc aacgccagaa acgacataac aatggaaggg gacactttct ataataaagt
      841 tcgattttat ggtaccaact ttcccgccaa tggtccagtt atgcagaaga agacgctgaa
      901 atgggagccc tccactgaga aaatgtatgt gcgtgatgga gtgctgacgg gtgatattga
      961 gatggctttg ttgcttgaag gaaatgccca ttaccgatgt gacttcagaa ctacttacaa
     1021 agctaaggag aagggtgtca agttaccagg cgcccacttt gtggaccact gcattgagat
     1081 tttaagccat gacaaagatt acaacaaggt taagctgtat gagcatgctg ttgctcattc
     1141 tggattgcct gacaatgcca gacgataagc ggccgcgact ctagatcata atcagccata
     1201 ccacat
//
