LOCUS       sequence-88620-e        2503 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 507 / 572, Emission: 516 
            / 580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2503)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos3.2-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..2503
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1773..2453
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 ctaccggact cagatctcga gctcaagctt cgaattccat ggcggccccc cgcccgtctc
       61 ccgcgatccc cgtttcggtc tcggctccgg ctttttacgc cccgcagaag aagttcggcc
      121 ctgtggtggc cccaaagccc aaagtgaatc ccttccggcc cagggacagc gagcctcccc
      181 cggcacccgg ggcccagcgc gcacagatgg gccgggtggg cgatattccc ccgccgcccc
      241 cggaagactt tcccctgcct ccacctcccc ttgctgggga tggcgacgat gcagagggtg
      301 ctctgggagg tgccttcccg ccgccccctc ccccgatcga ggaatcattt ccccctgcgc
      361 ctctggagga ggagatcttc ccttccccgc cgcctcctcc ggaggaggag ggagggcctg
      421 aggcccccat accgccccca ccacagccca gggagaaggt gagcagtatt gatttggaga
      481 tcgactctct gtcctcactg ctggatgaca tgaccaagaa tgatcctttc aaagcccggg
      541 tgtcatctgg atatgtgccc ccaccagtgg ccactccatt cagttccaag tccagtacca
      601 agcctgcagc cgggggcaca gcacccctgc ctccttggaa gtccccttcc agctcccagc
      661 ctctgcccca ggttccggct ccggctcaga gccagacaca gttccatgtt cagccccagc
      721 cccagcccaa gcctcaggtc caactccatg tccagtccca gacccagcct gtgtctttgg
      781 ctaacaccca gccccgaggg cccccagcct catctccggc tccagcccct aagttttctc
      841 cagtgactcc taagtttact cctgtggctt ccaagttcag tcctggagcc ccaggtggat
      901 ctgggtcaca accaaatcaa aaattggggc accccgaagc tctttctgct ggcacaggct
      961 cccctcaacc tcccagcttc acctatgccc agcagaggga gaagccccga gtgcaggaga
     1021 agcagcaccc cgtgccccca ccggctcaga accaaaacca ggtgcgctcc cctggggccc
     1081 cagggcccct gactctgaag gaggtggagg agctggagca gctgacccag cagctaatgc
     1141 aggacatgga gcatcctcag aggcagaatg tggctgtcaa cgaactctgc ggccgatgcc
     1201 atcaacccct ggcccgggcg cagccagccg tccgcgctct agggcagctg ttccacatcg
     1261 cctgcttcac ctgccaccag tgtgcgcagc agctccaggg ccagcagttc tacagtctgg
     1321 agggggcgcc gtactgcgag ggctgttaca ctgacaccct ggagaagtgt aacacctgcg
     1381 gggagcccat cactgaccgc atgctgaggg ccacgggcaa ggcctatcac ccgcactgct
     1441 tcacctgtgt ggtctgcgcc cgccccctgg agggcacctc cttcatcgtg gaccaggcca
     1501 accggcccca ctgtgtcccc gactaccaca agcagtacgc cccgaggtgc tccgtctgct
     1561 ctgagcccat catgcctgag cctggccgag atgagactgt gcgagtggtc gccctggaca
     1621 agaacttcca catgaagtgt tacaagtgtg aggactgcgg gaagcccctg tcgattgagg
     1681 cagatgacaa tggctgcttc cccctggacg gtcacgtgct ctgtcggaag tgccacactg
     1741 ctagagccca gacggatcca ccggtcgcca ccatgagtgc gattaagcca gacatgaaga
     1801 tcaaactccg tatggaaggc aacgtaaacg ggcaccactt tgtgatcgac ggagatggta
     1861 caggcaagcc ttttgaggga aaacagagta tggatcttga agtcaaagag ggcggacctc
     1921 tgccttttgc ctttgatatc ctgaccactg cattccatta cggcaacagg gtattcgcca
     1981 aatatccaga caacatacaa gactatttta agcagtcgtt tcctaagggg tattcgtggg
     2041 aacgaagctt gactttcgaa gacgggggca tttgcaacgc cagaaacgac ataacaatgg
     2101 aaggggacac tttctataat aaagttcgat tttatggtac caactttccc gccaatggtc
     2161 cagttatgca gaagaagacg ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg
     2221 atggagtgct gacgggtgat attgagatgg ctttgttgct tgaaggaaat gcccattacc
     2281 gatgtgactt cagaactact tacaaagcta aggagaaggg tgtcaagtta ccaggcgccc
     2341 actttgtgga ccactgcatt gagattttaa gccatgacaa agattacaac aaggttaagc
     2401 tgtatgagca tgctgttgct cattctggat tgcctgacaa tgccagacga taagcggccg
     2461 cgactctaga tcataatcag ccataccaca tgtagaagtg gcc
//
