LOCUS       sequence-88629-e        2116 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2116)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4a-Cx43-7
FEATURES             Location/Qualifiers
     source          1..2116
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1391..1400
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1397..2080
                     /codon_start=1
                     /product="fixation-resistant green-to-red photoswitchable 
                     fluorescent protein (Paez-Segala et al., 2015)"
                     /label=mEos4a
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICYA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 gcgctaccgg actcagatct cgagctcaag cttcgaattc ggcagcactt ttctttcatt
       61 ggggggaagg cgtgaggaaa gtaccaaaca gcagcagact tttaaacttt aaacagacag
      121 gtctgagagc ctgaactctc atttttcctt tgacttcagc ctccaaggag ttccaccaac
      181 tttggcgcgc cggcttcact ttcattaagt gaaagagagg tgcccagaca tgggtgactg
      241 gagtgccttg gggaagcttc tggacaaggt ccaagcctac tccaccgctg gagggaaggt
      301 gtggctgtca gtgctcttca tattcagaat cctgctcctg gggacagctg ttgagtcagc
      361 ttggggtgat gaacagtctg cctttcgctg taacactcaa caacctggct gcgaaaacgt
      421 ctgctatgac aagtccttcc ccatctctca cgtgcgcttc tgggtccttc agatcatatt
      481 cgtgtctgtg cccacactcc tgtacttggc ccatgtgttc tatgtgatga ggaaggaaga
      541 gaagctaaac aagaaagaag aggagctcaa agtggcccag actgacgggg tcaacgtgga
      601 gatgcacctg aagcagattg aaatcaagaa gttcaagtac gggattgaag agcacggcaa
      661 ggtgaaaatg aggggcggct tgctgagaac ctacatcatc agcatcctct tcaagtctgt
      721 cttcgaggtg gccttcctgc tcatccagtg gtacatctat gggttcagct tgagcgcggt
      781 ctacacctgc aagagagatc cctgccccca ccaggtagac tgcttcctct cacgtcccac
      841 ggagaaaacc atcttcatca tcttcatgct ggtggtgtcc ttggtgtctc tcgctttgaa
      901 catcattgag ctcttctacg tcttcttcaa aggcgttaag gatcgcgtga agggaagaag
      961 cgatccttac cacgccacca ctggcccact gagcccatca aaagactgcg gatctccaaa
     1021 atacgcctac ttcaatggct gctcctcacc aacggctcca ctctcgccta tgtctcctcc
     1081 tgggtacaag ctggttactg gtgacagaaa caattcctcg tgccgcaatt acaacaagca
     1141 agctagcgag caaaactggg cgaactacag cgcagagcaa aatcgcatgg ggcaggccgg
     1201 aagcaccatc tccaactcgc acgcccagcc gttcgatttc cccgacgaca accagaatgc
     1261 caaaaaagtt gctgctggac atgaactcca gccattagcc atcgtggacc aacgaccttc
     1321 cagcagagcc agcagccgcg ccagcagcag gcctcggcct gatgacctgg agattgcgga
     1381 tccaccggtc gccaccatgg tgagtgcgat taagccagac atgaggatca aactccgtat
     1441 ggaaggcaac gtaaacgggc accactttgt gatcgacgga gatggtacag gcaagcctta
     1501 tgagggaaaa cagaccatgg atcttgaagt caaagagggc ggacctctgc cttttgcctt
     1561 tgatatcctg accactgcat tccattacgg caacagggta ttcgttaaat atccagacaa
     1621 catacaagac tattttaagc agtcgtttcc taaggggtat tcgtgggaac gaagcttgac
     1681 tttcgaagac gggggcattt gctatgccag aaacgacata acaatggaag gggacacttt
     1741 ctataataaa gttcgatttt atggtaccaa ctttcccgcc aatggtccag ttatgcagaa
     1801 gaagacgctg aaatgggagc cctccactga gaaaatgtat gtgcgtgatg gagtgctgac
     1861 gggtgatatt catatggctt tgttgcttga aggaaatgcc cattaccgat gtgacttcag
     1921 aactacttac aaagctaagg agaagggtgt caagttacca ggctaccact ttgtggacca
     1981 cgccattgag attttaagcc atgacaaaga ttacaacaag gttaagctgt atgagcatgc
     2041 tgttgctcat tctggattgc ctgacaatgc cagacgataa gcggccgcga ctctagatca
     2101 taatcagcca taccac
//
