LOCUS       sequence-88630-e        1210 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1210)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4a-H2B-6
FEATURES             Location/Qualifiers
     source          1..1210
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             91..468
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      481..490
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             487..1170
                     /codon_start=1
                     /product="fixation-resistant green-to-red photoswitchable 
                     fluorescent protein (Paez-Segala et al., 2015)"
                     /label=mEos4a
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICYA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 ggccatgctt gtgacgtcag atccgctagc gctaccggac tcagatctcg agctcaagct
       61 tcgaattctg cagtcgacgg taccgccacc atgccagagc cagcgaagtc tgctcccgcc
      121 ccgaaaaagg gctccaagaa ggcggtgact aaggcgcaga agaaaggcgg caagaagcgc
      181 aagcgcagcc gcaaggagag ctattccatc tatgtgtaca aggttctgaa gcaggtccac
      241 cctgacaccg gcatttcgtc caaggccatg ggcatcatga attcgtttgt gaacgacatt
      301 ttcgagcgca tcgcaggtga ggcttcccgc ctggcgcatt acaacaagcg ctcgaccatc
      361 acctccaggg agatccagac ggccgtgcgc ctgctgctgc ctggggagtt ggccaagcac
      421 gccgtgtccg agggtactaa ggccatcacc aagtacacca gcgctaagga tccaccggtc
      481 gccaccatgg tgagtgcgat taagccagac atgaggatca aactccgtat ggaaggcaac
      541 gtaaacgggc accactttgt gatcgacgga gatggtacag gcaagcctta tgagggaaaa
      601 cagaccatgg atcttgaagt caaagagggc ggacctctgc cttttgcctt tgatatcctg
      661 accactgcat tccattacgg caacagggta ttcgttaaat atccagacaa catacaagac
      721 tattttaagc agtcgtttcc taaggggtat tcgtgggaac gaagcttgac tttcgaagac
      781 gggggcattt gctatgccag aaacgacata acaatggaag gggacacttt ctataataaa
      841 gttcgatttt atggtaccaa ctttcccgcc aatggtccag ttatgcagaa gaagacgctg
      901 aaatgggagc cctccactga gaaaatgtat gtgcgtgatg gagtgctgac gggtgatatt
      961 catatggctt tgttgcttga aggaaatgcc cattaccgat gtgacttcag aactacttac
     1021 aaagctaagg agaagggtgt caagttacca ggctaccact ttgtggacca cgccattgag
     1081 attttaagcc atgacaaaga ttacaacaag gttaagctgt atgagcatgc tgttgctcat
     1141 tctggattgc ctgacaatgc cagacgataa gcggccgcga ctctagatca taatcagcca
     1201 taccacattt
//
