LOCUS       sequence-88638-e        2487 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2487)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4a-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..2487
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1769..1778
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1775..2458
                     /codon_start=1
                     /product="fixation-resistant green-to-red photoswitchable 
                     fluorescent protein (Paez-Segala et al., 2015)"
                     /label=mEos4a
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICYA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 cgctaccgga ctcagatctc gagctcaagc ttcgaattcc atggcggccc cccgcccgtc
       61 tcccgcgatc cccgtttcgg tctcggctcc ggctttttac gccccgcaga agaagttcgg
      121 ccctgtggtg gccccaaagc ccaaagtgaa tcccttccgg cccagggaca gcgagcctcc
      181 cccggcaccc ggggcccagc gcgcacagat gggccgggtg ggcgatattc ccccgccgcc
      241 cccggaagac tttcccctgc ctccacctcc ccttgctggg gatggcgacg atgcagaggg
      301 tgctctggga ggtgccttcc cgccgccccc tcccccgatc gaggaatcat ttccccctgc
      361 gcctctggag gaggagatct tcccttcccc gccgcctcct ccggaggagg agggagggcc
      421 tgaggccccc ataccgcccc caccacagcc cagggagaag gtgagcagta ttgatttgga
      481 gatcgactct ctgtcctcac tgctggatga catgaccaag aatgatcctt tcaaagcccg
      541 ggtgtcatct ggatatgtgc ccccaccagt ggccactcca ttcagttcca agtccagtac
      601 caagcctgca gccgggggca cagcacccct gcctccttgg aagtcccctt ccagctccca
      661 gcctctgccc caggttccgg ctccggctca gagccagaca cagttccatg ttcagcccca
      721 gccccagccc aagcctcagg tccaactcca tgtccagtcc cagacccagc ctgtgtcttt
      781 ggctaacacc cagccccgag ggcccccagc ctcatctccg gctccagccc ctaagttttc
      841 tccagtgact cctaagttta ctcctgtggc ttccaagttc agtcctggag ccccaggtgg
      901 atctgggtca caaccaaatc aaaaattggg gcaccccgaa gctctttctg ctggcacagg
      961 ctcccctcaa cctcccagct tcacctatgc ccagcagagg gagaagcccc gagtgcagga
     1021 gaagcagcac cccgtgcccc caccggctca gaaccaaaac caggtgcgct cccctggggc
     1081 cccagggccc ctgactctga aggaggtgga ggagctggag cagctgaccc agcagctaat
     1141 gcaggacatg gagcatcctc agaggcagaa tgtggctgtc aacgaactct gcggccgatg
     1201 ccatcaaccc ctggcccggg cgcagccagc cgtccgcgct ctagggcagc tgttccacat
     1261 cgcctgcttc acctgccacc agtgtgcgca gcagctccag ggccagcagt tctacagtct
     1321 ggagggggcg ccgtactgcg agggctgtta cactgacacc ctggagaagt gtaacacctg
     1381 cggggagccc atcactgacc gcatgctgag ggccacgggc aaggcctatc acccgcactg
     1441 cttcacctgt gtggtctgcg cccgccccct ggagggcacc tccttcatcg tggaccaggc
     1501 caaccggccc cactgtgtcc ccgactacca caagcagtac gccccgaggt gctccgtctg
     1561 ctctgagccc atcatgcctg agcctggccg agatgagact gtgcgagtgg tcgccctgga
     1621 caagaacttc cacatgaagt gttacaagtg tgaggactgc gggaagcccc tgtcgattga
     1681 ggcagatgac aatggctgct tccccctgga cggtcacgtg ctctgtcgga agtgccacac
     1741 tgctagagcc cagacggatc caccggtcgc caccatggtg agtgcgatta agccagacat
     1801 gaggatcaaa ctccgtatgg aaggcaacgt aaacgggcac cactttgtga tcgacggaga
     1861 tggtacaggc aagccttatg agggaaaaca gaccatggat cttgaagtca aagagggcgg
     1921 acctctgcct tttgcctttg atatcctgac cactgcattc cattacggca acagggtatt
     1981 cgttaaatat ccagacaaca tacaagacta ttttaagcag tcgtttccta aggggtattc
     2041 gtgggaacga agcttgactt tcgaagacgg gggcatttgc tatgccagaa acgacataac
     2101 aatggaaggg gacactttct ataataaagt tcgattttat ggtaccaact ttcccgccaa
     2161 tggtccagtt atgcagaaga agacgctgaa atgggagccc tccactgaga aaatgtatgt
     2221 gcgtgatgga gtgctgacgg gtgatattca tatggctttg ttgcttgaag gaaatgccca
     2281 ttaccgatgt gacttcagaa ctacttacaa agctaaggag aagggtgtca agttaccagg
     2341 ctaccacttt gtggaccacg ccattgagat tttaagccat gacaaagatt acaacaaggt
     2401 taagctgtat gagcatgctg ttgctcattc tggattgcct gacaatgcca gacgataagc
     2461 ggccgcgact ctagatcata atcagcc
//
