LOCUS       sequence-88639-e        1936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1936)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-Actin-C-18
FEATURES             Location/Qualifiers
     source          1..1936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..715
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agtgcgatta agccagacat
       61 gaggatcaaa ctccgtatgg aaggcaacgt aaacgggcac cactttgtga tcgacggaga
      121 tggtacaggc aagccttatg agggaaaaca gaccatggat cttgaagtca aagagggcgg
      181 acctctgcct tttgcctttg atatcctgac cactgcattc cattacggca acagggtatt
      241 cgtgaaatat ccagacaaca tacaagacta ttttaagcag tcgtttccta aggggtattc
      301 gtgggaacga agcttgactt tcgaagacgg gggcatttgc aatgccagaa acgacataac
      361 aatggaaggg gacactttct ataataaagt tcgattttat ggtaccaact ttcccgccaa
      421 tggtccagtt atgcagaaga agacgctgaa atgggagccc tccactgaga aaatgtatgt
      481 gcgtgatgga gtgctgacgg gtgatattga gatggctttg ttgcttgaag gaaatgccca
      541 ttaccgatgt gacttcagaa ctacttacaa agctaaggag aagggtgtca agttaccagg
      601 cgcccacttt gtggaccacg ccattgagat tttaagccat gacaaagatt acaacaaggt
      661 taagctgtat gagcatgctg ttgctcattc tggattgcct gacaatgcca gacgatccgg
      721 actcagatct ggcagcggtg gaggcagcgc atccggcgga agcggaagcg atgatgatat
      781 cgccgcgctc gtcgtcgaca acggctccgg catgtgcaag gccggcttcg cgggcgacga
      841 tgccccccgg gccgtcttcc cctccatcgt ggggcgcccc aggcaccagg gcgtgatggt
      901 gggcatgggt cagaaggatt cctatgtggg cgacgaggcc cagagcaaga gaggcatcct
      961 caccctgaag taccccatcg agcacggcat cgtcaccaac tgggacgaca tggagaaaat
     1021 ctggcaccac accttctaca atgagctgcg tgtggctccc gaggagcacc ccgtgctgct
     1081 gaccgaggcc cccctgaacc ccaaggccaa ccgcgagaag atgacccaga tcatgtttga
     1141 gaccttcaac accccagcca tgtacgttgc tatccaggct gtgctatccc tgtacgcctc
     1201 tggccgtacc actggcatcg tgatggactc cggtgacggg gtcacccaca ctgtgcccat
     1261 ctacgagggg tatgccctcc cccatgccat cctgcgtctg gacctggctg gccgggacct
     1321 gactgactac ctcatgaaga tcctcaccga gcgcggctac agcttcacca ccacggccga
     1381 gcgggaaatc gtgcgtgaca ttaaggagaa gctgtgctac gtcgccctgg acttcgagca
     1441 agagatggcc acggctgctt ccagctcctc cctggagaag agctacgagc tgcctgacgg
     1501 ccaggtcatc accattggca atgagcggtt ccgctgccct gaggcactct tccagccttc
     1561 cttcctgggc atggagtcct gtggcatcca cgaaactacc ttcaactcca tcatgaagtg
     1621 tgacgtggac atccgcaaag acctgtacgc caacacagtg ctgtctggcg gcaccaccat
     1681 gtaccctggc attgccgaca ggatgcagaa ggagatcact gccctggcac ccagcacaat
     1741 gaagatcaag atcattgctc ctcctgagcg caagtactcc gtgtggatcg gcggctccat
     1801 cctggcctcg ctgtccacct tccagcagat gtggatcagc aagcaggagt atgacgagtc
     1861 cggcccctcc atcgtccacc gcaaatgctt ctagggatcc accggatcta gataactgat
     1921 cataatcagc catacc
//
