LOCUS       sequence-88640-e        1752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1752)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-Annexin-12
FEATURES             Location/Qualifiers
     source          1..1752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..715
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agtgcgatta agccagacat
       61 gaggatcaaa ctccgtatgg aaggcaacgt aaacgggcac cactttgtga tcgacggaga
      121 tggtacaggc aagccttatg agggaaaaca gaccatggat cttgaagtca aagagggcgg
      181 acctctgcct tttgcctttg atatcctgac cactgcattc cattacggca acagggtatt
      241 cgtgaaatat ccagacaaca tacaagacta ttttaagcag tcgtttccta aggggtattc
      301 gtgggaacga agcttgactt tcgaagacgg gggcatttgc aatgccagaa acgacataac
      361 aatggaaggg gacactttct ataataaagt tcgattttat ggtaccaact ttcccgccaa
      421 tggtccagtt atgcagaaga agacgctgaa atgggagccc tccactgaga aaatgtatgt
      481 gcgtgatgga gtgctgacgg gtgatattga gatggctttg ttgcttgaag gaaatgccca
      541 ttaccgatgt gacttcagaa ctacttacaa agctaaggag aagggtgtca agttaccagg
      601 cgcccacttt gtggaccacg ccattgagat tttaagccat gacaaagatt acaacaaggt
      661 taagctgtat gagcatgctg ttgctcattc tggattgcct gacaatgcca gacgatccgg
      721 actcagatct cgagctcaag cttcgaattc tatggccatg gcaaccaaag gaggtactgt
      781 caaagctgct tcaggattca atgccatgga agatgcccag accctgagga aggccatgaa
      841 agggctcggc accgatgaag acgccattat tagcgtcctt gcctaccgca acaccgccca
      901 gcgccaggag atcaggacag cctacaagag caccatcggc agggacttga tagacgacct
      961 gaagtcagaa ctgagtggca acttcgagca ggtgattgtg gggatgatga cgcccacggt
     1021 gctgtatgac gtgcaagagc tgcgaagggc catgaaggga gccggcactg atgagggctg
     1081 cctaattgag atcctggcct cccggacccc tgaggagatc cggcgcataa gccaaaccta
     1141 ccagcagcaa tatggacgga gccttgaaga tgacattcgc tctgacacat cgttcatgtt
     1201 ccagcgagtg ctggtgtctc tgtcagctgg tgggagggat gaaggaaatt atctggacga
     1261 tgctctcgtg agacaggatg cccaggacct gtatgaggct ggagagaaga aatgggggac
     1321 agatgaggtg aaatttctaa ctgttctctg ttcccggaac cgaaatcacc tgttgcatgt
     1381 gtttgatgaa tacaaaagga tatcacagaa ggatattgaa cagagtatta aatctgaaac
     1441 atctggtagc tttgaagatg ctctgctggc tatagtaaag tgcatgagga acaaatctgc
     1501 atattttgct gaaaagctct ataaatcgat gaagggcttg ggcaccgatg ataacaccct
     1561 catcagagtg atggtttctc gagcagaaat tgacatgttg gatatccggg cacacttcaa
     1621 gagactctat ggaaagtctc tgtactcgtt catcaagggt gacacatctg gagactacag
     1681 gaaagtactg cttgttctct gtggaggaga tgattaagga tccaccggat ctagataact
     1741 gatcataatc ag
//
