LOCUS       sequence-88643-e        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-Caveolin-C-10
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      263..272
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             269..952
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 ttaccgcttg ctgtctgccc tctttggcat cccgatggca ctcatctggg gcatttactt
       61 cgccattctc tctttcctgc acatctgggc agttgtacca tgcattaaga gcttcctgat
      121 tgagattcag tgcatcagcc gtgtctattc catctacgtc cacaccgtct gtgacccact
      181 ctttgaagct gttgggaaaa tattcagcaa tgtccgcatc aacttgcaga aagaaataag
      241 ctccggaggg gatccaccgg tcgccaccat ggtgagtgcg attaagccag acatgaggat
      301 caaactccgt atggaaggca acgtaaacgg gcaccacttt gtgatcgacg gagatggtac
      361 aggcaagcct tatgagggaa aacagaccat ggatcttgaa gtcaaagagg gcggacctct
      421 gccttttgcc tttgatatcc tgaccactgc attccattac ggcaacaggg tattcgtgaa
      481 atatccagac aacatacaag actattttaa gcagtcgttt cctaaggggt attcgtggga
      541 acgaagcttg actttcgaag acgggggcat ttgcaatgcc agaaacgaca taacaatgga
      601 aggggacact ttctataata aagttcgatt ttatggtacc aactttcccg ccaatggtcc
      661 agttatgcag aagaagacgc tgaaatggga gccctccact gagaaaatgt atgtgcgtga
      721 tggagtgctg acgggtgata ttgagatggc tttgttgctt gaaggaaatg cccattaccg
      781 atgtgacttc agaactactt acaaagctaa ggagaagggt gtcaagttac caggcgccca
      841 ctttgtggac cacgccattg agattttaag ccatgacaaa gattacaaca aggttaagct
      901 gtatgagcat gctgttgctc attctggatt gcctgacaat gccagacgat aagcggccgc
      961 gactctagat cataatcagc cataccacat gtagaagtat tgcac
//
