LOCUS       sequence-88644-e        2133 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2133)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-Cx43-7
FEATURES             Location/Qualifiers
     source          1..2133
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1403..1412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1409..2092
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 tcagatccgc tagcgctacc ggactcagat ctcgagctca agcttcgaat tcggcagcac
       61 ttttctttca ttggggggaa ggcgtgagga aagtaccaaa cagcagcaga cttttaaact
      121 ttaaacagac aggtctgaga gcctgaactc tcatttttcc tttgacttca gcctccaagg
      181 agttccacca actttggcgc gccggcttca ctttcattaa gtgaaagaga ggtgcccaga
      241 catgggtgac tggagtgcct tggggaagct tctggacaag gtccaagcct actccaccgc
      301 tggagggaag gtgtggctgt cagtgctctt catattcaga atcctgctcc tggggacagc
      361 tgttgagtca gcttggggtg atgaacagtc tgcctttcgc tgtaacactc aacaacctgg
      421 ctgcgaaaac gtctgctatg acaagtcctt ccccatctct cacgtgcgct tctgggtcct
      481 tcagatcata ttcgtgtctg tgcccacact cctgtacttg gcccatgtgt tctatgtgat
      541 gaggaaggaa gagaagctaa acaagaaaga agaggagctc aaagtggccc agactgacgg
      601 ggtcaacgtg gagatgcacc tgaagcagat tgaaatcaag aagttcaagt acgggattga
      661 agagcacggc aaggtgaaaa tgaggggcgg cttgctgaga acctacatca tcagcatcct
      721 cttcaagtct gtcttcgagg tggccttcct gctcatccag tggtacatct atgggttcag
      781 cttgagcgcg gtctacacct gcaagagaga tccctgcccc caccaggtag actgcttcct
      841 ctcacgtccc acggagaaaa ccatcttcat catcttcatg ctggtggtgt ccttggtgtc
      901 tctcgctttg aacatcattg agctcttcta cgtcttcttc aaaggcgtta aggatcgcgt
      961 gaagggaaga agcgatcctt accacgccac cactggccca ctgagcccat caaaagactg
     1021 cggatctcca aaatacgcct acttcaatgg ctgctcctca ccaacggctc cactctcgcc
     1081 tatgtctcct cctgggtaca agctggttac tggtgacaga aacaattcct cgtgccgcaa
     1141 ttacaacaag caagctagcg agcaaaactg ggcgaactac agcgcagagc aaaatcgcat
     1201 ggggcaggcc ggaagcacca tctccaactc gcacgcccag ccgttcgatt tccccgacga
     1261 caaccagaat gccaaaaaag ttgctgctgg acatgaactc cagccattag ccatcgtgga
     1321 ccaacgacct tccagcagag ccagcagccg cgccagcagc aggcctcggc ctgatgacct
     1381 ggagattgcg gatccaccgg tcgccaccat ggtgagtgcg attaagccag acatgaggat
     1441 caaactccgt atggaaggca acgtaaacgg gcaccacttt gtgatcgacg gagatggtac
     1501 aggcaagcct tatgagggaa aacagaccat ggatcttgaa gtcaaagagg gcggacctct
     1561 gccttttgcc tttgatatcc tgaccactgc attccattac ggcaacaggg tattcgtgaa
     1621 atatccagac aacatacaag actattttaa gcagtcgttt cctaaggggt attcgtggga
     1681 acgaagcttg actttcgaag acgggggcat ttgcaatgcc agaaacgaca taacaatgga
     1741 aggggacact ttctataata aagttcgatt ttatggtacc aactttcccg ccaatggtcc
     1801 agttatgcag aagaagacgc tgaaatggga gccctccact gagaaaatgt atgtgcgtga
     1861 tggagtgctg acgggtgata ttgagatggc tttgttgctt gaaggaaatg cccattaccg
     1921 atgtgacttc agaactactt acaaagctaa ggagaagggt gtcaagttac caggcgccca
     1981 ctttgtggac cacgccattg agattttaag ccatgacaaa gattacaaca aggttaagct
     2041 gtatgagcat gctgttgctc attctggatt gcctgacaat gccagacgat aagcggccgc
     2101 gactctagat cataatcagc cataccacat tgt
//
