LOCUS       sequence-88645-e        1327 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1327)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..1327
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    534..586
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      594..603
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             600..1283
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 tgaaccgttc agatccgcta gccaccatgg gccccaagcg gcggcagctg acgttccggg
       61 agaagtcgcg gatcatccag gaggtggagg agaacccgga cctacgcaag ggcgagatcg
      121 cgcggcgctt caacatcccg ccgtccacgc tgagcacgat cctgaagaac aagcgcgcca
      181 tcctggcgtc ggagcgcaag tacggggtgg cctccacctg ccgcaagacc aacaagctgt
      241 ctccctacga caagctcgag ggcttgctca tcgcctggtt ccagcagatc cgcgccgccg
      301 gcctgccggt caagggcatc atcctcaagg agaaggcgct gcgcatagcc gaggagctgg
      361 gcatggacga cttcaccgcc tccaacggct ggctggaccg cttccgccgg cgccacggcg
      421 tggtgtcctg cagcggcgtg gcccgcgccc gcgcgcgaaa cgctgccccc cgcaccccgg
      481 cggcgcctgc cagtccggcc gcggtgccct cggagggcag tggcgggagc actctcgagc
      541 tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg gtcgccacca
      601 tggtgagtgc gattaagcca gacatgagga tcaaactccg tatggaaggc aacgtaaacg
      661 ggcaccactt tgtgatcgac ggagatggta caggcaagcc ttatgaggga aaacagacca
      721 tggatcttga agtcaaagag ggcggacctc tgccttttgc ctttgatatc ctgaccactg
      781 cattccatta cggcaacagg gtattcgtga aatatccaga caacatacaa gactatttta
      841 agcagtcgtt tcctaagggg tattcgtggg aacgaagctt gactttcgaa gacgggggca
      901 tttgcaatgc cagaaacgac ataacaatgg aaggggacac tttctataat aaagttcgat
      961 tttatggtac caactttccc gccaatggtc cagttatgca gaagaagacg ctgaaatggg
     1021 agccctccac tgagaaaatg tatgtgcgtg atggagtgct gacgggtgat attgagatgg
     1081 ctttgttgct tgaaggaaat gcccattacc gatgtgactt cagaactact tacaaagcta
     1141 aggagaaggg tgtcaagtta ccaggcgccc actttgtgga ccacgccatt gagattttaa
     1201 gccatgacaa agattacaac aaggttaagc tgtatgagca tgctgttgct cattctggat
     1261 tgcctgacaa tgccagacga taagcggccg cgactctaga tcataatcag ccatacccac
     1321 attgtag
//
