LOCUS       sequence-88646-e        1287 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Clathrin Vesicles, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1287)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-Clathrin-15
FEATURES             Location/Qualifiers
     source          1..1287
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             45..725
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 ccatgctagt gacgtcagat ccgctagcgc taccggtcgc caccatggtg agtgcgatta
       61 agccagacat gaggatcaaa ctccgtatgg aaggcaacgt aaacgggcac cactttgtga
      121 tcgacggaga tggtacaggc aagccttatg agggaaaaca gaccatggat cttgaagtca
      181 aagagggcgg acctctgcct tttgcctttg atatcctgac cactgcattc cattacggca
      241 acagggtatt cgtgaaatat ccagacaaca tacaagacta ttttaagcag tcgtttccta
      301 aggggtattc gtgggaacga agcttgactt tcgaagacgg gggcatttgc aatgccagaa
      361 acgacataac aatggaaggg gacactttct ataataaagt tcgattttat ggtaccaact
      421 ttcccgccaa tggtccagtt atgcagaaga agacgctgaa atgggagccc tccactgaga
      481 aaatgtatgt gcgtgatgga gtgctgacgg gtgatattga gatggctttg ttgcttgaag
      541 gaaatgccca ttaccgatgt gacttcagaa ctacttacaa agctaaggag aagggtgtca
      601 agttaccagg cgcccacttt gtggaccacg ccattgagat tttaagccat gacaaagatt
      661 acaacaaggt taagctgtat gagcatgctg ttgctcattc tggattgcct gacaatgcca
      721 gacgatccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac atggctgatg
      781 actttggctt cttctcgtcg tcggagagtg gtgccccgga ggcggcggag gaggacccgg
      841 cggccgcctt cctggcccag cagagagcga gattgcaggc atagagaacg acgagggctt
      901 cggggcacct gccggcagcc atgcggcccc cgcacagccg ggccccacga gtggggctgg
      961 ttctgaggac atggggacca cagtcaatgg agatgtgttt caggaggcca acggtcctgc
     1021 tgatggctac gcagccattg cccaggctga caggctgacc caggagcctg aaagcatccg
     1081 cagtgggcga gaaggagcag aggaaacggc tgcagagctg gagcttgcat ctaaggtcac
     1141 ggaaccagaa tggcggggaa gccacaagga cctgcgagga gtggaacacg ccgagtgaca
     1201 gttagaaaca aatcaaacgg catcagagag ctttcgagat caggggaccc agcaaaggga
     1261 agtgcaccat gtgattcaac cagagca
//
